Fabrication of chitosan-coated mixed spinel ferrite integrated with graphene oxide (GO) for magnetic extraction of viral RNA for potential detection of SARS-CoV-2

Singh, Vijay Kumar; Batoo, Khalid Mujasam; Singh, M.

doi:10.1007/s00339-021-05067-7

Cited by 13 publications

(2 citation statements)

References 71 publications

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“…Transmission electron microscopy (TEM) provides detailed information on the shape, and size distribution of the nanoparticles (Shi et al, 2012). Atomic force microscopy (AFM) is a high‐resolution imaging technique that can provide information on the surface topography, roughness, and mechanical properties of nanoparticles (Shi et al, 2012), X‐ray diffraction (XRD) can provide information on the crystallinity, lattice parameters, and peak positions of the nanoparticles (Hoque et al, 2016; Singh et al, 2021). In the following paragraphs, some critical factors that need to be considered to assess whether a desirable formulation has been achieved or not are discussed in details.…”

Section: Physiochemical Characterization Of Nps After Cs Adsorptionmentioning

confidence: 99%

Chitosan‐coated nanoparticles in innovative cancer bio‐medicine

Rezaei,

Zarkesh,

Fotouhi

et al. 2024

Drug Development Research

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In the recent decade, nanoparticles (NPs) have had enormous implications in cancer biomedicine, including research, diagnosis, and therapy. However, their broad application still faces obstacles due to some practical limitations and requires further development. Recently, there has been more interest in the coated class of nanoparticles to address those challenges. Chitosan‐coated NPs are simple to produce, biodegradable, biocompatible, exhibit antibacterial activity, and have less cytotoxicity. This study provides an updated and comprehensive overview of the application of chitosan‐coated NPs as a promising class of NPs in cancer biomedicine. Additionally, we discussed chitosan‐coated lipid, metal, and polymer‐based nanoparticles in biomedical applications. Furthermore, different coating methods and production/characterization procedures were reviewed. Moreover, the biological and physicochemical advantages of chitosan‐coated NPs, including facilitated controlled release, greater physicochemical stability, improved cell/tissue interaction, and enhanced bioavailability of medications, were highlighted. Finally, the prospects of chitosan‐coated NPs in cancer biomedicine were discussed.

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Section: Physiochemical Characterization Of Nps After Cs Adsorptionmentioning

confidence: 99%

Chitosan‐coated nanoparticles in innovative cancer bio‐medicine

Rezaei,

Zarkesh,

Fotouhi

et al. 2024

Drug Development Research

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“…Furthermore, the studies also did not employ indirect sequence-specific magneto-enrichment despite its superior analytical sensitivity (Table S1). In fact, none of the published magneto-extraction assisted NAAT methods for detecting SARS-CoV-2 has recruited either a sequence-specific capture (direct or indirect) in combination with or without an electrochemical readout 34,[38][39][40][41][42][43] . Therefore, the modality of a sequence-specific indirect magnetoenrichment of pathogen nucleic acid (SARS-CoV-2 or otherwise) from a complex biofluid or host nucleic acid-containing sample with downstream LAMP, either with fluorescence or electrochemical readout, remains unexplored.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Evaluation of Indirect Sequence-Specific Magnetoextraction-aided Fluorescence and Electrochemical LAMP with SARS-CoV-2 Nucleic Acid as the Analyte

Tripathy

Agarkar

Talukdar

et al. 2022

Preprint

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Since the beginning of the SARS-CoV-2 pandemic, nucleic acid amplification test (NAAT) such as quantitative real-time reverse transcriptase PCR (qRT-PCR) has remained the primary intervention for diagnostics and containment of SARS-CoV-2. Despite its remarkable clinical as well as analytical specificity and sensitivity, qRT-PCR necessitates pure nucleic acid free of any polymerase inhibitors (from complex biological matrices) as its substrate. Similarly, isothermal NAATs (iNAATs), despite their advantage over qRT-PCR in terms of thermal cycler independence, still require pure nucleic acid as a template. The requirement of pure nucleic acid in turn warrants the use of spin-column mediated extraction with centralized high-speed centrifuges. Additionally, utilization of centralized real-time fluorescence readout and use of sequence-specific molecular probes like TaqMan further prevent their deployment in decentralized locations. To circumvent these disadvantages, we have envisioned a sample-to-answer workflow comprising of indirect sequence-specific magneto-extraction (also referred to as magnetocapture, magneto-preconcentration, or magneto-enrichment in this manuscript) followed by in situ fluorescence or electrochemical LAMP. This study, using SARS-CoV-2 nucleic acid as the analyte, compared the analytical effectiveness of indirect and direct sequence-specific magneto-extraction followed by LAMP. Since contamination carryover may affect the efficacy of sequence-specific indirect magnetocapture, its performance in presence of excess host nucleic acid or serum was probed. Through these experiments, we have established a comprehensive limited resource-adoptable and highly specific nucleic acid detection method with the limit of detection of 2.5 copies/L. Its advantage lies in the flexibility of using either centralized real-time SYBR-based fluorescence LAMP or portable electrochemical LAMP as the readout. Simultaneously, the performance with magneto-capture aided fluorescence and electrochemical LAMP readouts were weighed against each other in terms of analytical sensitivity, specificity, and turnaround time. Additionally, the analytical efficacy of the magnetocapture-LAMP workflow was also checked against that of LAMP using pure nucleic acid as a template. Besides being the first report utilizing electrochemical LAMP to detect SARS-CoV-2 nucleic acid, this would probably be the first study to make the analytical comparative assessment of magnetic preconcentration combined with in situ fluorescence and electrochemical LAMP. It is probably also the first study (to the best of our knowledge) to compare the analytical efficacy of a sequence-specific magnetic target enrichment-LAMP (fluorescence and electrochemical) to that of a LAMP assay using pure nucleic acid as a template.

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Progress and Outlooks in Designing Photonic Biosensor for Virus Detection

Tsalsabila,

Dabur,

Budiarso

et al. 2024

Advanced Optical Materials

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The recent outbreak of coronavirus disease 2019 (COVID‐19) highlights the critical need for rapid, sensitive, and accurate virus detection methods to prevent and manage pandemics. Among the available sensing methods, photonic biosensors have emerged as a forefront technology, characterized by their high sensitivity, minimal analyte requirements, and suitability for miniaturization, making them ideal for point‐of‐care applications in virus detection. This review comprehensively summarizes the recent progress of photonic biosensor technologies, focusing on wavelength shift and luminescence‐based mechanisms. Their operational principles, general configurations, and the challenges associated with these technologies are looked into. An overview of the material developments used in photonic biosensors, encompassing organic, inorganic, and hybrid composite‐based materials is further presented. The discussion extends to surface functionalization using biorecognition elements, including DNA/RNA, aptamers, and antibodies, to craft the specificity of the photonic biosensors for viruses. Ultimately, the importance of a multidisciplinary approach is emphasized in developing new materials architecture, biological receptors, and modifications to photonic methods, aiming to realize better biosensors for virus detection with ultra‐high sensitivity, rapid response, and excellent selectivity.

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Fabrication of chitosan-coated mixed spinel ferrite integrated with graphene oxide (GO) for magnetic extraction of viral RNA for potential detection of SARS-CoV-2

Cited by 13 publications

References 71 publications

Chitosan‐coated nanoparticles in innovative cancer bio‐medicine

Chitosan‐coated nanoparticles in innovative cancer bio‐medicine

A Comparative Evaluation of Indirect Sequence-Specific Magnetoextraction-aided Fluorescence and Electrochemical LAMP with SARS-CoV-2 Nucleic Acid as the Analyte

Progress and Outlooks in Designing Photonic Biosensor for Virus Detection

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