Fabrication of chitosan nanoparticles loaded with aromatase inhibitors for the advancement of gonadal development in Clarias magur (Hamilton, 1822)

Wisdom, K S; Bhat, Irfan Ahmad; Kumar, Pravesh; Pathan, Mujahid Khan; Chanu, Thongam Ibemcha; Walke, Pravin S.; Sharma, Rupam

doi:10.1016/j.aquaculture.2018.07.049

Cited by 20 publications

(11 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, CSNPs are effective encapsulators and carriers and can be used for oral delivery of vaccines and bioactive ingredients owing to their high resistant to intestinal degradation. They are able to protect the encapsulated ingredients, extend their shelf life, and enhance their intestinal absorption without cytotoxicity [ 24 , 25 ]. However, more in vivo investigations are still required to evaluate the immunomodulation combined with the antimicrobial efficacy of dietary CSNPs for prevention and/or treatment combating fish diseases.…”

Section: Introductionmentioning

confidence: 99%

Dietary Chitosan Nanoparticles: Potential Role in Modulation of Rainbow Trout (Oncorhynchus mykiss) Antibacterial Defense and Intestinal Immunity against Enteric Redmouth Disease

et al. 2021

View full text Add to dashboard Cite

Bio-nanotechnology employing bio-sourced nanomaterial is an emerging avenue serving the field of fish medicine. Marine-sourced chitosan nanoparticles (CSNPs) is a well-known antimicrobial and immunomodulatory reagent with low or no harm side effects on fish or their human consumers. In this study, in vitro skin mucus and serum antibacterial activity assays along with intestinal histology, histochemical, and gene expression analyses were performed to evaluate the impact of dietary CSNPs (5 g kg−1 dry feed) on rainbow trout resistance against ‘enteric redmouth’ disease. Two treatment conditions were included; short-term prophylactic-regimen for 21 days before the bacterial challenge, and long-term therapeutic-regimen for 21 days before the challenge and extended for 28 days after the challenge. Our results revealed higher antibacterial defense ability and positive intestinal histochemical and molecular traits of rainbow trout after dietary CSNPs. The prophylactic-regimen improved trout health while the therapeutic regimen improved their disease resistance and lowered their morbidity. Therefore, it is anticipated that CSNPs is an effective antibacterial and immunomodulatory fish feed supplement against the infectious threats. However, the CSNPs seem to be more effective in the therapeutic application rather than being used for short-term prophylactic applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Dietary Chitosan Nanoparticles: Potential Role in Modulation of Rainbow Trout (Oncorhynchus mykiss) Antibacterial Defense and Intestinal Immunity against Enteric Redmouth Disease

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Chitosan conjugated AIs (letrozole and fadrozole) increased the reproductive output of C. magur , by increasing the testosterone levels, GSI (Gonadosomatic Index) and number of spermatozoa in testes (Wisdom et al . 2018). Similarly, during sexual maturation, fadrozole was found to be responsible for increased spermiation in Caho salmon ( Oncorhynchus kisutch ) (Afonso Luis et al .…”

Section: Genes Involved In Steroidogenesis Pathway and Their Modulation In Fishmentioning

confidence: 99%

Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Bhat

Dar

Ahmad

et al. 2021

Reviews in Aquaculture

Self Cite

View full text Add to dashboard Cite

In fish, as in higher animals, the normal ontogenesis of the gonads is the basis for successful reproduction. Gonadal differentiation and development depend on the genetic cascade that programmes the events. Eventually, gonads become the sites for the maturation of gametes, which are originally derived from the primordial germ cells (PGCs) and form the cellular basis of reproduction. In fish hormones of the hypothalamic-pituitary-gonadal (HPG) axis regulate reproduction and fertility by promoting or inhibiting the production of gonadal sex steroids and their function. Gonadal sex steroids not only guide proper gonadal development and function but also play a key role in the activation and regulation of the HPG axis through two feedback control systems. In this review, we discuss the general outline and expression pattern of the major genes involved in testicular development and gonadal steroidogenesis in fish. The review attempts, for the first time, to collect and discuss recent information regarding the regulation of sex differentiation and steroidogenic genes during the stages of testicular development and in response to different external factors. Furthermore, clear insights into the important genes and their regulation involved in the HPG axis and spermatogenesis are presented briefly. This review can serve as a guide for fish breeders on the substances that have a positive impact on reproduction in males.

show abstract

“…Several studies evaluated chitosan nanoparticles loading aromatase inhibitors and eurycomanone, compounds that promote gonadal development. Chitosan-mediated delivery of aromatase inhibitors and eurycomanone prolonged serum presence, improved testicular development with lack of testicular toxicity, and led to higher serum concentrations of reproductive hormones [125][126][127][128].…”

Section: Chitosan Loading Chemical Compoundsmentioning

confidence: 99%

Chitosan-Based Drug Delivery System: Applications in Fish Biotechnology

Rashidpour

Pablos

et al. 2020

Polymers

View full text Add to dashboard Cite

Chitosan is increasingly used for safe nucleic acid delivery in gene therapy studies, due to well-known properties such as bioadhesion, low toxicity, biodegradability and biocompatibility. Furthermore, chitosan derivatization can be easily performed to improve the solubility and stability of chitosan–nucleic acid polyplexes, and enhance efficient target cell drug delivery, cell uptake, intracellular endosomal escape, unpacking and nuclear import of expression plasmids. As in other fields, chitosan is a promising drug delivery vector with great potential for the fish farming industry. This review highlights state-of-the-art assays using chitosan-based methodologies for delivering nucleic acids into cells, and focuses attention on recent advances in chitosan-mediated gene delivery for fish biotechnology applications. The efficiency of chitosan for gene therapy studies in fish biotechnology is discussed in fields such as fish vaccination against bacterial and viral infection, control of gonadal development and gene overexpression and silencing for overcoming metabolic limitations, such as dependence on protein-rich diets and the low glucose tolerance of farmed fish. Finally, challenges and perspectives on the future developments of chitosan-based gene delivery in fish are also discussed.

show abstract

Fabrication of chitosan nanoparticles loaded with aromatase inhibitors for the advancement of gonadal development in Clarias magur (Hamilton, 1822)

Cited by 20 publications

References 49 publications

Dietary Chitosan Nanoparticles: Potential Role in Modulation of Rainbow Trout (Oncorhynchus mykiss) Antibacterial Defense and Intestinal Immunity against Enteric Redmouth Disease

Dietary Chitosan Nanoparticles: Potential Role in Modulation of Rainbow Trout (Oncorhynchus mykiss) Antibacterial Defense and Intestinal Immunity against Enteric Redmouth Disease

Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Chitosan-Based Drug Delivery System: Applications in Fish Biotechnology

Contact Info

Product

Resources

About