Fabrication of conductive gelatin methacrylate–polyaniline hydrogels

Wu, Yibo; Chen, Yong X.; Yan, Jiahan; Quinn, D.F.; Dong, Ping; Sawyer, Stephen W.; Soman, Pranav

doi:10.1016/j.actbio.2016.01.036

Cited by 101 publications

(88 citation statements)

References 67 publications

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“…[17,[26][27][28] Previously, we have reported that PANi can be integrated with synthetic PEGDA and naturally derived gelatin methacrylate (GelMA) hydrogels in situ in order to develop 3D conductive-hydrogels that are sufficiently biocompatible with seeded cells. [29,30] However, like studies similar to ours, the harsh processing methods were incompatible with cell encapsulation approaches. In this study, we developed a conductive PANi-GelMA composite by incorporating PANi clusters within a photo-polymerizable GelMA hydrogel and investigate the ability of encapsulated osteoblast-like cells to mineralize within the hydrogel matrix.…”

Section: Introductionmentioning

confidence: 90%

“…[43] Additionally, GelMA can be processed into useful structures using UV-crosslinking, an easy-to-use processing method which has been shown to not cause lasting damage to encapsulated cells in short exposures. [29] Furthermore, due to the relative ease with which the hydrogel can be synthesized and cured, the addition of PANi and cells post processing make GelMA an optimal medium for the creation of GelMA-PANi-Saos-2 composites.…”

Section: Discussionmentioning

confidence: 99%

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Conductive gelatin methacrylate-poly(aniline) hydrogel for cell encapsulation

Sawyer

Dong

Venn

et al. 2017

Biomed. Phys. Eng. Express

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New conductive hydrogels with superior biocompatibility continue to be developed in order to serve as bioactive scaffolds capable of modulating cellular functionality for tissue engineering applications. We developed an electrically conductive gelatin methacrylate-poly(aniline) (GelMA-PANi) hydrogel that is permissive of matrix mineralization by encapsulated osteoblast-like cells. Incorporation of PANi clusters within the GelMA matrix increases the electro-conductivity of the composite gel, while maintaining the osteoid-like soft mechanical properties that allows three-dimensional encapsulation of living cells. Viability of human osteogenic cells encapsulated within GelMA-PANi hydrogels was similar to that of GelMA. Cells within GelMA-PANi also demonstrated the capability of depositing mineral within the hydrogel matrix after being chemically induced for two weeks, although the total mineral content was lower as compared to GelMA. Additionally, we demonstrated that the GelMA-PANi-composite hydrogel could be printed in complex, user-defined geometries using digital projection stereolithography.

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Section: Introductionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Conductive gelatin methacrylate-poly(aniline) hydrogel for cell encapsulation

Sawyer

Dong

Venn

et al. 2017

Biomed. Phys. Eng. Express

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“…They are widely used for several biomedical applications due to their resemblance to the native extracellular matrix (ECM), as well as their remarkable biocompatibility, and tunable mechanical and biochemical properties 2, 3 . However, hydrogels are typically non-conductive, which limits their application as bioactive scaffolds for excitable tissues, such as neural, as well as cardiac, and skeletal muscle tissues 4 . More recently, hydrogels with electroconductive properties have been developed through the incorporation of different nanomaterials (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…gold nanoparticles, silver nanowires, carbon nanotubes, graphene oxide (GO)) and conductive polymers (e.g. polyaniline, polypyrrole (PPy), polythiophene) to their network 5–11 . These electroconductive hydrogels (ECHs) constitute a class of smart biomaterials that combine the electrical properties of intrinsically conductive elements, with highly hydrated, and biocompatible hydrogel networks 1 .…”

Section: Introductionmentioning

confidence: 99%

“…Despite the wide application of ECHs and conductive polymers for biomedical applications, they still exhibit major technical limitations, including the absence of cell binding sites, cytotoxicity, as well as poor solubility, processability, and biodegradability 5–11 . In addition, conventional strategies used to synthesize ECHs often suffer from difficult and prolonged processing, harsh reaction conditions, and difficulties in the incorporation of the conductive materials, and modulation of their physical properties.…”

Section: Introductionmentioning

confidence: 99%

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Engineering Biodegradable and Biocompatible Bio-ionic Liquid Conjugated Hydrogels with Tunable Conductivity and Mechanical Properties

Noshadi

Walker

Portillo-Lara

et al. 2017

Sci Rep

125

109

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Conventional methods to engineer electroconductive hydrogels (ECHs) through the incorporation of conductive nanomaterials and polymers exhibit major technical limitations. These are mainly associated with the cytotoxicity, as well as poor solubility, processability, and biodegradability of their components. Here, we describe the engineering of a new class of ECHs through the functionalization of non-conductive polymers with a conductive choline-based bio-ionic liquid (Bio-IL). Bio-IL conjugated hydrogels exhibited a wide range of highly tunable physical properties, remarkable in vitro and in vivo biocompatibility, and high electrical conductivity without the need for additional conductive components. The engineered hydrogels could support the growth and function of primary cardiomyocytes in both two dimentinal (2D) and three dimensional (3D) cultures in vitro. Furthermore, they were shown to be efficiently biodegraded and possess low immunogenicity when implanted subcutaneously in rats. Taken together, our results suggest that Bio-IL conjugated hydrogels could be implemented and readily tailored to different biomedical and tissue engineering applications.

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Electrically Conductive and 3D‐Printable Oxidized Alginate‐Gelatin Polypyrrole:PSS Hydrogels for Tissue Engineering

Distler

Polley

Shi

et al. 2021

Adv Healthcare Materials

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Electroactive hydrogels can be used to influence cell response and maturation by electrical stimulation. However, hydrogel formulations which are 3D printable, electroactive, cytocompatible, and allow cell adhesion, remain a challenge in the design of such stimuli‐responsive biomaterials for tissue engineering. Here, a combination of pyrrole with a high gelatin‐content oxidized alginate‐gelatin (ADA‐GEL) hydrogel is reported, offering 3D‐printability of hydrogel precursors to prepare cytocompatible and electrically conductive hydrogel scaffolds. By oxidation of pyrrole, electroactive polypyrrole:polystyrenesulfonate (PPy:PSS) is synthesized inside the ADA‐GEL matrix. The hydrogels are assessed regarding their electrical/mechanical properties, 3D‐printability, and cytocompatibility. It is possible to prepare open‐porous scaffolds via bioplotting which are electrically conductive and have a higher cell seeding efficiency in scaffold depth in comparison to flat 2D hydrogels, which is confirmed via multiphoton fluorescence microscopy. The formation of an interpenetrating polypyrrole matrix in the hydrogel matrix increases the conductivity and stiffness of the hydrogels, maintaining the capacity of the gels to promote cell adhesion and proliferation. The results demonstrate that a 3D‐printable ADA‐GEL can be rendered conductive (ADA‐GEL‐PPy:PSS), and that such hydrogel formulations have promise for cell therapies, in vitro cell culture, and electrical‐stimulation assisted tissue engineering.

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Fabrication of conductive gelatin methacrylate–polyaniline hydrogels

Cited by 101 publications

References 67 publications

Conductive gelatin methacrylate-poly(aniline) hydrogel for cell encapsulation

Conductive gelatin methacrylate-poly(aniline) hydrogel for cell encapsulation

Engineering Biodegradable and Biocompatible Bio-ionic Liquid Conjugated Hydrogels with Tunable Conductivity and Mechanical Properties

Electrically Conductive and 3D‐Printable Oxidized Alginate‐Gelatin Polypyrrole:PSS Hydrogels for Tissue Engineering

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