Fabrication of DNAzyme-functionalized hydrogel and its application for visible detection of circulating tumor DNA

Mao, Xiaoxia; Pan, Shaobing; Zhou, Duoqi; Huang, Huajun; Zhang, Yuanguang

doi:10.1016/j.snb.2019.01.076

Cited by 45 publications

(20 citation statements)

References 26 publications

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“…Alternative approaches such as liquid biopsies may provide a solution. 134 To this end, DNAzymes have been proposed as valuable tools to analyze tumor biomarkers in liquid biopsies for the detection of clinically relevant targets, including exosomes, 135 circulating tumor cells (CTCs), 136 circulating tumor DNA, 137 and microRNA (miRNA). 138 For the purpose of this review, we focus on three target categories most widely studied in DNAzyme systems: CTCs, intracellular markers, and miRNA.…”

Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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Since their discovery almost three decades ago, DNAzymes have been used extensively in biosensing. Depending on the type of DNAzyme being used, these functional oligonucleotides can act as molecular recognition elements within biosensors, offering high specificity to their target analyte, or as reporters capable of transducing a detectable signal. Several parameters need to be considered when designing a DNAzyme-based biosensor. In particular, given that many of these biosensors immobilize DNAzymes onto a sensing surface, selecting an appropriate immobilization strategy is vital. Suboptimal immobilization can result in both DNAzyme detachment and poor accessibility toward the target, leading to low sensing accuracy and sensitivity. Various approaches have been employed for DNAzyme immobilization within biosensors, ranging from amine and thiol-based covalent attachment to non-covalent strategies involving biotin–streptavidin interactions, DNA hybridization, electrostatic interactions, and physical entrapment. While the properties of each strategy inform its applicability within a proposed sensor, the selection of an appropriate strategy is largely dependent on the desired application. This is especially true given the diverse use of DNAzyme-based biosensors for the detection of pathogens, metal ions, and clinical biomarkers. In an effort to make the development of such sensors easier to navigate, this paper provides a comprehensive review of existing immobilization strategies, with a focus on their respective advantages, drawbacks, and optimal conditions for use. Next, common applications of existing DNAzyme-based biosensors are discussed. Last, emerging and future trends in the development of DNAzyme-based biosensors are discussed, and gaps in existing research worthy of exploration are identified.

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Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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“…Polymeric materials used for sensors are divided into natural hydrogels, derived from natural polymers, e.g., proteins (collagen [ 76 , 80 , 83 ], elastin [ 84 , 85 ], gelatin [ 86 , 87 , 88 ], albumin [ 29 , 89 ]), polysaccharides (hyaluronic acid [ 90 ], alginate [ 91 , 92 , 93 , 94 , 95 ], chitosan [ 82 , 96 , 97 , 98 , 99 , 100 , 101 ], cellulose [ 74 ]), and synthetic hydrogels [ 39 , 102 , 103 , 104 , 105 , 106 ], based on laboratory-made polymers. Hydrogels in sensing applications are often used as a hybrid material, blend of polymers or in composition with inorganic materials, e.g., graphene, graphene oxide, or silica [ 31 ].…”

Section: Hydrogel Materials In Sensingmentioning

confidence: 99%

“…Considering the wide range of characteristics that can be achieved by tuning in both molecular and structural levels and the ability to respond to external stimuli, such as temperature [ 58 , 65 ], light [ 66 ], pH [ 58 , 94 ], ionic strength [ 62 , 127 , 135 ], and the presence of (bio)molecules [ 104 , 136 , 137 , 138 , 139 ], synthetic hydrogels have become important materials for the design and construction of sensors and biosensors in various fields of applications. Different types of synthetic polymer-based hydrogels have been used in sensing, e.g., poly(acrylic acid) [ 40 , 105 , 140 ], poly(ethylene glycol) [ 36 , 39 , 141 , 142 ], poly(ethylene glycol) methacrylate [ 143 ], poly(acrylic acid- co -dimethylaminoethyl methacrylate) [ 144 ], poly(methyl methacrylate- co -methacrylic acid) [ 145 ], polyacrylamide [ 35 , 37 , 77 , 103 ], poly(acrylamide- co -acrylic acid) [ 146 ], poly(N,N-dimethylacrylamide) [ 78 ], poly(N,N-dimethylacrylamide- co -2-(dimethylmaleimido)N-ethyl-acrylamide- co -vinyl-4,4-dimethylazlactone) [ 102 , 106 ], poly(N-isopropylacrylamide- co -2-acrylamido-2-methylpropane sulfonic acid) [ 147 ], poly(vinyl alcohol) [ 81 ], poly(2-hydroxyethyl methacrylate) [ 75 ], and poly(diallyldimethyl ammonium chloride) [ 75 ].…”

Section: Hydrogel Materials In Sensingmentioning

confidence: 99%

Shaping Macromolecules for Sensing Applications—From Polymer Hydrogels to Foldamers

et al. 2022

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Sensors are tools for detecting, recognizing, and recording signals from the surrounding environment. They provide measurable information on chemical or physical changes, and thus are widely used in diagnosis, environment monitoring, food quality checks, or process control. Polymers are versatile materials that find a broad range of applications in sensory devices for the biomedical sector and beyond. Sensory materials are expected to exhibit a measurable change of properties in the presence of an analyte or a stimulus, characterized by high sensitivity and selectivity of the signal. Signal parameters can be tuned by material features connected with the restriction of macromolecule shape by crosslinking or folding. Gels are crosslinked, three-dimensional networks that can form cavities of different sizes and forms, which can be adapted to trap particular analytes. A higher level of structural control can be achieved by foldamers, which are macromolecules that can attain well-defined conformation in solution. By increasing control over the three-dimensional structure, we can improve the selectivity of polymer materials, which is one of the crucial requirements for sensors. Here, we discuss various examples of polymer gels and foldamer-based sensor systems. We have classified and described applied polymer materials and used sensing techniques. Finally, we deliberated the necessity and potential of further exploration of the field towards the increased selectivity of sensory devices.

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“…In addition, by using the DNAzyme activity of G-quadruplex/hemin, the hydrogel catalyzed the oxidation-reduction reaction of ABTS and hydrogen peroxide to yield turquoise oxidized ABTS*. This method exhibited great sensitivity for ctDNA with an LOD of 0.32 pM [38]. Recently, a chemiluminescent biosensor for the detection of adenosine was designed with a detection limit as low as 0.104 pM ( Fig.…”

Section: Dnazyme-based Dna Hydrogelsmentioning

confidence: 99%

DNA hydrogel-empowered biosensing

Khajouei

Ravan

Ebrahimi

2020

Advances in Colloid and Interface Science

100

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DNA hydrogels as special members in the DNA nanotechnology have provided crucial prerequisites to create innovative gels owing to their sufficient stability, biocompatibility, biodegradability, and tunable multifunctionality. These properties have tailored DNA hydrogels for various applications in drug delivery, tissue engineering, sensors, and cancer therapy. Recently, DNA-based materials have attracted substantial consideration for the exploration of smart hydrogels, in which their properties can change in response to chemical or physical stimuli. In other words, these gels can undergo switchable gel-to-sol or sol-to-gel transitions upon application of different triggers. Moreover, various functional motifs like i-motif structures, antisense DNAs, DNAzymes, and aptamers can be inserted into the polymer network to offer a molecular recognition capability to the complex. In this manuscript, a comprehensive discussion will be endowed with the recognition capability of different kinds of DNA hydrogels and the alternation in physicochemical behaviors upon target introducing. Finally, we offer a vision into the future landscape of DNA based hydrogels in sensing applications.

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Fabrication of DNAzyme-functionalized hydrogel and its application for visible detection of circulating tumor DNA

Cited by 45 publications

References 26 publications

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

Shaping Macromolecules for Sensing Applications—From Polymer Hydrogels to Foldamers

DNA hydrogel-empowered biosensing

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