Fabrication of Electrochemical Immunosensor for Detection of Interleukin 8 Biomarker via Layer‐by‐layer Self‐assembly Process on Cost‐effective Fluorine Tin Oxide Electrode

Aydın, Elif Burcu; Sezgintürk, Mustafa Kemal

doi:10.1002/elan.202060603

Cited by 7 publications

(4 citation statements)

References 39 publications

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“…The immunosensor was applied to spiked human saliva. Among the various electrochemical biosensors reported by Prof. Sezgintürk’s group for the determination of IL-8 [ 104 , 105 , 106 ], Figure 7 D displays a scheme of the biosensor prepared using an ITO electrode modified with 6-phosphonohexanoic acid (PHA) for the immobilization of anti-IL-8 and impedimetric detection [ 105 ]. The interest in using phosphonic acids in the preparation of electrochemical biosensors derives from their ability to spontaneously produce self-assembled monolayers (SAMs) on different electrode surfaces, including metal oxides.…”

Section: Oral Microbiomementioning

confidence: 99%

Electrochemical (Bio)Sensing Devices for Human-Microbiome-Related Biomarkers

Sánchez‐Tirado

Agüı́

González‐Cortés

et al. 2023

Sensors

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The study of the human microbiome is a multidisciplinary area ranging from the field of technology to that of personalized medicine. The possibility of using microbiota biomarkers to improve the diagnosis and monitoring of diseases (e.g., cancer), health conditions (e.g., obesity) or relevant processes (e.g., aging) has raised great expectations, also in the field of bioelectroanalytical chemistry. The well-known advantages of electrochemical biosensors—high sensitivity, fast response, and the possibility of miniaturization, together with the potential for new nanomaterials to improve their design and performance—position them as unique tools to provide a better understanding of the entities of the human microbiome and raise the prospect of huge and important developments in the coming years. This review article compiles recent applications of electrochemical (bio)sensors for monitoring microbial metabolites and disease biomarkers related to different types of human microbiome, with a special focus on the gastrointestinal microbiome. Examples of electrochemical devices applied to real samples are critically discussed, as well as challenges to be faced and where future developments are expected to go.

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Section: Oral Microbiomementioning

confidence: 99%

Electrochemical (Bio)Sensing Devices for Human-Microbiome-Related Biomarkers

Sánchez‐Tirado

Agüı́

González‐Cortés

et al. 2023

Sensors

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show abstract

“…The immunosensor was applied to spiked human saliva. Among the various electrochemical biosensors reported by Prof. Sezgintürk´s group for the determination of IL-8 [102][103][104], Figure 7D displays a scheme of the biosensor prepared using an ITO electrode modified with 6-phosphonohexanoic acid (PHA) for the immobilization of anti-IL-8, and impedimetric detection [103]. The interest of using phosphonic acids in the preparation of electrochemical biosensors derives from their ability to spontaneously produce self-assembled monolayers (SAMs) onto different electrode surfaces including metal oxides.…”

Section: Oral Microbiomementioning

confidence: 99%

Electrochemical (Bio)Sensing Devices for Human Microbiome Related Biomarkers

Sánchez‐Tirado¹,

Agüı́²,

González‐Cortés³

et al. 2022

Preprint

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The study of the human microbiome is a multidisciplinary area that affects from the field of technology to that of personalized medicine. The possibility of using microbiota biomarkers for the improvement in the diagnosis and monitoring of diseases (e.g. cancer), health conditions (e.g. obesity) or clinical parameters (e.g. ageing) has aroused great expectations, also in the scope of bio-electroanalytical chemistry. The well-known advantages of electrochemical transduction: high sensitivity, rapid response and possibility of miniaturization, together with the potential of new nanomaterials to improve both the design of electrode platforms and the performance of the resulting electrochemical biosensors make presumably a huge development in the next few years providing a better understanding of human microbiome entities. In this review article, the recent applications of electrochemical (bio)sensors for monitoring microbial metabolites and biomarkers of diseases related to different types of the human microbiome, with a special focus on the gastrointestinal microbiome, are reviewed. Examples of electrochemical devices applied to real samples are critically discussed as well as the challenges and prospects to be faced.

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“…According to statistics, there are more than three hundred thousand cases of oral cancer tumor diagnosed worldwide annually. [1][2][3][4] Presently, the survival ration of this cancer is still very low owing to the failure from treatment modalities. One of the main causes is the great challenge in achieving the early diagnosis.…”

Section: Introductionmentioning

confidence: 99%

“…Because of the high worldwide pervasiveness, oral cancer has been listed in the eight most frequent and malignant cancer by World Health Organization (WHO). According to statistics, there are more than three hundred thousand cases of oral cancer tumor diagnosed worldwide annually [1–4] . Presently, the survival ration of this cancer is still very low owing to the failure from treatment modalities.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Sensitive Electrochemical Sandwich‐Like Immunosensor for Interleukin‐8 Utilizing Carbon Black as a Sensing Platform and Silver‐Ferrocene as a Signal Amplifier

et al. 2023

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Designing sensitive and low-cost method for interleukin-8 (IL-8) determination is of great significance in the early diagnosis of oral cancer. In the present work, a simple, low-cost and sensitive sandwich-like electrochemical immunosensor of IL-8 was developed by introducing perylene tetracarboxylic acidcarbon black (PTCA-CB) as sensing platform and silverferrocene (AgÀ Fc) as signal amplifier. PTCA-CB can offer large surface area and high conductivity as well as the stable supporter to assemble primary antibody (Ab 1 ), while Ag could carry Fc probe and secondary antibodies (Ab 2 ) to produce Ab 2 À AgÀ Fc signal amplifier. In the presence of IL-8 with different concentrations, the peak currents from the formed sandwich-like structure Ab 2 À AgÀ Fc/IL-8/Ab 1 /CB can increase with the increase of IL-8 concentration, a sensitive and low-cost electrochemical immunosensor for IL-8 was thus constructed based on this principle. After optimizing the related control conditions, a wide linear range (3.0 pg mL À 1 to 2.0 ng mL À 1 ) and very low detection limit (1.0 pg mL À 1 ) were achieved, it's thus convinced the developed sensor has important clinical applications.

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Fabrication of Electrochemical Immunosensor for Detection of Interleukin 8 Biomarker via Layer‐by‐layer Self‐assembly Process on Cost‐effective Fluorine Tin Oxide Electrode

Cited by 7 publications

References 39 publications

Electrochemical (Bio)Sensing Devices for Human-Microbiome-Related Biomarkers

Electrochemical (Bio)Sensing Devices for Human-Microbiome-Related Biomarkers

Electrochemical (Bio)Sensing Devices for Human Microbiome Related Biomarkers

A Simple and Sensitive Electrochemical Sandwich‐Like Immunosensor for Interleukin‐8 Utilizing Carbon Black as a Sensing Platform and Silver‐Ferrocene as a Signal Amplifier

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