Fabrication of electrospun cellulose-derived nanofiber membranes with enhanced stability properties of arginase

Abstract

In this study, cellulose acetate (CA)/polyvinylpyrrolidone (PVP) and CA/PVP/Mn 2+ nanofibers were produced by the electrospinning method, and these cellulose-derived membranes were used as carriers for arginase immobilization for the first time. The structural and morphological analysis of these cellulose-derived nanofibers were determined by attenuated total reflection-Fourier to transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffraction (XRD), and scanning electron microscopy (SEM). After the immobilization process, it was observed that the thermal properties of the cellulose-derived nanofibers especially improved. The optimum temperature value for free arginase was found to be 35 °C, and this value was found to be 40 °C for arginase immobilized cellulose-derived nanofibers. When the free arginase retained only about 35% of its activity at 50 °C and 60 °C after 60 min, arginase immobilized nanofibers protected 65% of their activity under the same conditions. In addition, arginase immobilized CA/PVP and CA/PVP/Mn 2+ nanofibers managed to retain 50% of their activity even after 9 and 12 reuses, respectively.

An Alternative Approach to Plastic Recycling: Fabrication and Characterization of rPET/CA Nanofiber Carriers to Enhance Porcine Pancreatic Lipase Stability Properties

“…In the second stage, which is the main degradation step, it was observed that 88% of the mass was removed from the structure between 390 and 480 °C. It was stated in previous studies that the mass loss occurring in this step was due to the main thermal degradation reactions of CA chains. , In the last step, observed between 480 and 700 °C, approximately 93% of the initial mass was observed to move away from the structure. Additionally, it was determined that the maximum decomposition temperature of CA was 450 °C from the DTG curve.…”