Fabrication of Gehlenite Nanopowder Containing Electrospun Nanofibers for Bone Tissue Engineering

Abstract

Bioactive Polycaprolactone/Collagen nanofibers containing gehlenite Nano-powder were fabricated using electrospinning for bone tissue engineering. The physicochemical properties of scaffolds measured using scanning electron microscopy, tensile test, FTIR spectroscopy, and water contact angle measurement test. A slight enhancement (~20 nm) in fibers diameter was observed by adding gehlenite nanoparticles to the scaffolds, but the fiber diameter distributions for both scaffolds were within the normal range of extracellular matrix (50-500 nm). In vitro experiments demonstrated well attachment and higher proliferation of MG-63 cells on gehlenite nanoparticles containing scaffolds. Besides, the stimulatory cell differentiation effects of Polycaprolactone/Collagen/gehlenite scaffolds were demonstrated by Alizarin red staining. This study represents for advanced of the natural and synthetic polymers for bone tissue engineering.

Fabrication of porous gehlenite coating on Al2O3–ZrO2–SiC composite ceramics and its in vitro biological activities

“…It is believed that the Ca 2 Al 2 SiO 7 coating with porous structure and bioactive ions promotes the osteogenic differentiation. 29,30,32,35 Combined with SBF soaking test results, it can be concluded that the Ca 2 Al 2 SiO 7 coating has good bioactivity.

Figure 12.(a) Proliferation activity and (b) analysis of ALP activity of MC3T3-E1 cells seeded on coverslip (positive control) and S1–S3 for 1–5 days. “ns” indicates no significance, whereas different letters above bars indicate significant difference ( p < .05).

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“…It is believed that the Ca 2 Al 2 SiO 7 coating with porous structure and bioactive ions promotes the osteogenic differentiation. 29,30,32,35 Combined with SBF soaking test results, it can be concluded that the Ca 2 Al 2 SiO 7 coating has good bioactivity.…”

“…Previous studies have demonstrated that Ca 2 Al 2 SiO 7 could release trace bioactive ions (e.g., Ca 2+ and Si 4+ ) in tissue microenvironment, which favored the bone tissue regeneration. [24][25][26][27][28][29][30][31][32] Moreover, it was found that Ca 2 Al 2 SiO 7 has a very good self-forming ability of pores during sintering process, 33 and the rough and porous surface has important effect on cell behavior and tissue regeneration. [34][35][36][37] In addition, during the heat treatment of SiC-containing ceramics, CO gas bubbles resulted from the oxidation of SiC also favored the formation of porous SiO 2 [38][39][40] and the final porous Ca 2 Al 2 SiO 7 coating.…”