Fabrication of Gelatin Methacrylate (GelMA) Scaffolds with Nano- and Micro-Topographical and Morphological Features

Abstract

The design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to generate novel gelatin methacrylate (GelMA)-based scaffolds with nano and micro topographical and morphological features. First, electrospinning parameters and crosslinking processes were optimized to obtain electrospun nanofibrous scaffolds. GelMA mats were characterized by SEM, FTIR, DSC, TGA, contact angle, and water uptake. Various nanofibrous GelMA mats with defect-free fibers and stability in aqueous media were obtained. Then, micropatterned molds produced by photolithography were used as collectors in the electrospinning process. Thus, biocompatible GelMA nanofibrous scaffolds with micro-patterns that mimic extracellular matrix were obtained successfully by combining two micro/nanofabrication techniques, electrospinning, and micromolding. Taking into account the cell viability results, the methodology used in this study could be considered a valuable tool to develop patterned GelMA based nanofibrous scaffolds for cell culture and tissue engineering.

Poly-ε-Caprolactone/Gelatin Hybrid Electrospun Composite Nanofibrous Mats Containing Ultrasound Assisted Herbal Extract: Antimicrobial and Cell Proliferation Study

“…Electrospun nanofibers create an effective physical barrier at the wound region, thereby protect the open wound from further external damages and exogenous microorganisms. Since the structural features of electrospun scaffolds closely mimic the natural extracellular matrix (ECM) [12,13], they can serve as a template for cell adhesion, spreading, and proliferation supporting new tissue regeneration. The electrospun mats allow gaseous exchange, removal/release of wound exudates and maintain a moisturized environment to accelerate the wound healing process [14].…”

“…PCL exhibits many advantages such as biocompatibility, biodegradability, excellent processability, and desired mechanical properties [39]. Gelatin has good porosity, biocompatibility, fluid retention properties, cell-specific binding sites and non-antigenicity [13]. However, both the polymers individually are inadequate to fulfil their role as dressing materials due to their certain disadvantages.…”

Electrospun Gelatin Fibers Surface Loaded ZnO Particles as a Potential Biodegradable Antibacterial Wound Dressing

“…Gelatin, as a kind of natural material, is hydrolyzed from collagen in animals, which has many advantages, such as good accessibility, a wide source of raw materials and low cost. Its amino acid composition is similar to collagen and has good biocompatibility, biodegradability and low immunogenicity [7,8,9]. Especially in the past decades, with the development of electrospinning technology, gelatin fibers can be prepared simply and quickly.…”

14-3-3ε protein-loaded 3D hydrogels favor osteogenesis

“…GelMA (4%wt. ), synthesized as previously reported [11], and photoinitiator (0.5%wt. Irgacure2959) were then added to the AlgL and AlgH solutions and stirred overnight.…”