Fabrication of highly active phosphatase-like fluorescent cerium-doped carbon dots for <i>in situ</i> monitoring the hydrolysis of phosphate diesters

Du, Jinyan; Qi, Shuangqing; Chen, Juan; Yang, Ying; Fan, Tingting; Zhang, Ping; Zhuo, Shujuan; Zhu, Changqing

doi:10.1039/d0ra07429b

Cited by 15 publications

(12 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using bis(4-nitrophenyl)phosphate, the phosphatase-like activity of the Ce-doped carbon dots [157] was systematically investigated and the study showed that the fluorescent Cedoped carbon dots could promote the hydrolysis of a phosphate ester. Enzyme-mimicking nanomaterials can hydrolyze DNA, organophosphate, and their analogs for future gene editing and environmentally friendly treatment of pesticide residues.…”

Section: Phosphatase Mimicsmentioning

confidence: 99%

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Wang

Xianyu

2022

Small Methods

View full text Add to dashboard Cite

treatment of cancers, the second leading cause of death in the world that are hard to be treated in the terminal stages. [2] For instance, liver cancer is one of the most lethal cancers and has a poor prognosis with a 5-year survival rate of less than 20%. [3] The early detection and timely treatment of cancers are very important for the patients to save their lives. Microorganism contamination in food is the main cause of foodborne diseases and 70% of patients with foodborne illnesses get sick after taking foods or water contaminated with pathogenic microorganisms. [4] Viruses are one of the major causes of infectious diseases and various public health events like the acquired immunodeficiency syndrome (AIDS), the severe acute respiratory syndrome (SARS), and the coronavirus disease 2019 (COVID-19). [5] Therefore, the timely and rapid detection of microorganisms is of great importance to ensure human health and public safety. [6] In addition, the detections of small molecules including mycotoxins and exogenous pollutions play an important role in food safety. Mycotoxins such as aflatoxin B1, ochratoxin A, and tenuazonic acid are classified as naturally occurring carcinogens by the World Health Organization that are extremely harmful to both humans and animals. [7] They not only directly threaten human life by contaminating food, but also indirectly affect human health through the food chain by contaminating feeds of animals. [8,9] Commonly used immunoassays include enzyme-linked immunosorbent assay (ELISA), quantum dot fluorescence immunoassay, fluorescence polarization immunoassay, colloidal gold immunoassay, chemiluminescent immunoassay (CLIA), electrochemical immunoassay, and microarray-based immunoassay. In spite of the different formats of immunoassays, they share the same principle that relies on the specific binding between antigenic determinants and antibody recognition sites. Therefore, it is of great significance to prepare specific antibodies with high affinity towards the target molecules to improve the specificity of immunoassays. Conventional immunoassays generally use polyclonal and monoclonal antibodies, but both types of antibodies have limitations such as poor specificity, low stability, and complicated preparation. To meet these challenges, a new type of antibody called "nanobody" has emerged. Nanobody is the heavy-chain-only antibody with the advantages of small size, high specificity, mass expression and high stability, which can act as a substitute for conventional antibodies in immunoassays and a promising low-cost immuno-affinity detection reagent.Immunoassay as a rapid and convenient method for detecting a variety of targets has attracted tremendous interest with its high specificity and sensitivity. Among the commonly used immunoassays, enzyme-linked immunosorbent assay has been widely used as a gold standard method in various fields that consists of two main components including a recognition element and an enzyme label. With the rapid advances in nanotechnology, nanobodies and nanozym...

show abstract

Section: Phosphatase Mimicsmentioning

confidence: 99%

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Wang

Xianyu

2022

Small Methods

View full text Add to dashboard Cite

show abstract

“…To begin with, N-methylmorpholine abstracts protons from Ce-bound water (Ce-H 2 O) molecules in an alkaline (N-methylmorpholine buffer solution) environment, resulting in the formation of nucleophilic cerium hydroxide (Ce-OH) species. 57,58 In step 1, the oxygen atom (OvP-) of 4-NPP coordinates with the aqua-hydroxyl active species Ce metal centre as it contains vacant 4f orbitals to form an intermediate species, which is useful for converting an intermolecular reaction to an intramolecular reaction. Step 2 involves the attack of the phosphorous atom of the 4-NPP molecule by an intramolecular metal hydroxide, which aids the release of the 4-nitrophenolate ion from the surface of the Ce-MOF catalyst, which was monitored using UV-vis spectroscopy.…”

Section: Kinetic Studies Of the Hydrolysis Of 4-nppmentioning

confidence: 99%

“…In the rate-determining step, the surface Ce-bound water molecules and 4-NPP react with each other and 4-NP is released from the surface of the Ce-MOF catalyst (Fig. S15 †).To begin with, N-methylmorpholine abstracts protons from Ce-bound water (Ce-H 2 O) molecules in an alkaline (N-methylmorpholine buffer solution) environment, resulting in the formation of nucleophilic cerium hydroxide (Ce-OH) species 57,58. In step 1, the oxygen atom (OvP-) of 4-NPP coordinates with the aqua-hydroxyl active species Ce metal centre as it contains vacant 4f orbitals to form an intermediate species, which is useful for converting an intermolecular reaction to an intramolecular reaction.…”

mentioning

confidence: 99%

A Ce-MOF as an alkaline phosphatase mimic: Ce-OH₂ sites in catalytic dephosphorylation

Bhattacharjee

Chakraborty

Bhaumik

2022

Inorg. Chem. Front.

View full text Add to dashboard Cite

show abstract

“…fabricated Ce‐doped carbon dots (CeCDs) via a one‐step hydrothermal carbonization and investigated its mimetic phosphatase activity for phosphate ester hydrolysis cleavage. [ 161 ] The CeCdS exhibited good catalytic activity for promoting hydrolysis of bis(4‐nitrophenyl) phosphate (BNPP) and could also mimic phosphatase based on the Inner Filter Effect (IFE). Besides, there have been carbon‐based materials developed as SOD mimics for addressing the limitations of natural SOD.…”

Section: The Application Of Sustainable Carbon Materials In Catalysismentioning

confidence: 99%

Sustainable Carbon Materials toward Emerging Applications

Lan

Yang

et al. 2021

Small Methods

View full text Add to dashboard Cite

It is desirable for a sustainable society that the production and utilization of renewable materials are net‐zero in terms of carbon emissions. Carbon materials with emerging applications in CO2 utilization, renewable energy storage and conversion, and biomedicine have attracted much attention both academically and industrially. However, the preparation process of some new carbon materials suffers from energy consumption and environmental pollution issues. Therefore, the development of low‐cost, scalable, industrially and economically attractive, sustainable carbon material preparation methods are required. In this regard, the use of biomass and its derivatives as a precursor of carbon materials is a major feature of sustainability. Recent advances in the synthetic strategy of sustainable carbon materials and their emerging applications are summarized in this short review. Emphasis is made on the discussion of the original intentions and various sustainable strategies for producing sustainable carbon materials. This review provides basic insights and significant guidelines for the further design of sustainable carbon materials and their emerging applications in catalysis and the biomedical field.

show abstract

Fabrication of highly active phosphatase-like fluorescent cerium-doped carbon dots for in situ monitoring the hydrolysis of phosphate diesters

Abstract: The hydrolytic cleavage of BNPP was catalyzed and monitored by the fluorescent CeCDs.

Cited by 15 publications

References 71 publications

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

A Ce-MOF as an alkaline phosphatase mimic: Ce-OH₂ sites in catalytic dephosphorylation

Sustainable Carbon Materials toward Emerging Applications

Contact Info

Product

Resources

About

Fabrication of highly active phosphatase-like fluorescent cerium-doped carbon dots for in situ monitoring the hydrolysis of phosphate diesters

Abstract: The hydrolytic cleavage of BNPP was catalyzed and monitored by the fluorescent CeCDs.

Cited by 15 publications

References 71 publications

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

A Ce-MOF as an alkaline phosphatase mimic: Ce-OH2 sites in catalytic dephosphorylation

Sustainable Carbon Materials toward Emerging Applications

Contact Info

Product

Resources

About

A Ce-MOF as an alkaline phosphatase mimic: Ce-OH₂ sites in catalytic dephosphorylation