Fabrication of macroporous cement scaffolds using PEG particles: In vitro evaluation with induced pluripotent stem cell-derived mesenchymal progenitors

Sladkova, Martina; Palmer, Michael; Öhman, Caroline; Alhaddad, Rawan Jaragh; Esmael, Asmaa; Engqvist, Håkan; Peppo, Giuseppe Maria de

doi:10.1016/j.msec.2016.06.075

Cited by 16 publications

(22 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The initial number of cells in the construct, as well as their spatial distribution can influence migration, communication, and differentiation (Bitar et al, ) and affect the quality and regenerative properties of tissue‐engineered products. In this study, seeding on scaffolds using our optimized protocol (Sladkova et al, ) resulted in very high attachment and uniform distribution for both 1013A‐MP and BMSC1 cells and culminated the formation of a dense layer of cells covering the scaffolds after 7 weeks in culture. Comparable seeding enables objective assessment of the engineering potential of the CPC scaffolds seeded with 1013A‐MP and BMSC1 cells and in relation to decellularized bone.…”

Section: Discussionmentioning

confidence: 69%

“…At P4, 1013A‐MP and BMSC1 cells were detached, counted, and seeded onto the CPCs and decellularized bone scaffolds as previously described (Sladkova et al, ). Following seeding, the samples ( n = 22) were incubated overnight in expansion medium, and the day after, the medium was collected and unattached cells counted with a haemocytometer to estimate the seeding efficiency using the following equation:

Seeding efficiency = {\frac{Number of seeded cells - Number of unattached cells}{Number of seeded cells}}^{*} 100 %

…”

Section: Methodsmentioning

confidence: 99%

“…The expression of osteogenic genes was studied via real‐time polymerase chain reaction (PCR). Briefly, RNA was extracted using TRIzol (Thermo Scientific) and purified using the RNeasy Mini Kit (Qiagen) as previously described (Sladkova et al, ). Purified RNA was quantified and reversely transcribed with random hexamers using the GoScript™ Reverse Transcription System (Promega, Madison, WI) according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies in vitro have shown that macroporous CPC scaffolds (with different formulation) support cell viability and differentiation of human mesenchymal stem cells (MSCs; Vater et al, ; Weir & Xu, ), and grafts of CPC scaffolds seeded with human MSCs promote skeletal regeneration in animal models (Chen et al, ), with good prospects for future developments and clinical applications. To overcome the limitations associated with isolation and poor regeneration potential of adult stem cells, researchers have recently explored the possibility to use human pluripotent stem cells as source of mesenchymal progenitors for bone engineering applications (Idowu, Cama, Deb, & Di Silvio, ; J. Liu, Chen, Zhao, & Xu, ; X. Liu, Wang, et al, ; Sladkova et al, ; Wang et al, ). In particular, human induced pluripotent stem cells (iPSCs) can be derived from every patient, proliferate indefinitely, and give rise to all cells constituting the bone tissue (Takahashi et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…To overcome the limitations associated with isolation and poor regeneration potential of adult stem cells, researchers have recently explored the possibility to use human pluripotent stem cells as source of mesenchymal progenitors for bone engineering applications (Idowu, Cama, Deb, & Di Silvio, 2014;J. Liu, Chen, Zhao, & Xu, 2014;Sladkova et al, 2016;Wang et al, 2015). In particular, human induced pluripotent stem cells (iPSCs) can be derived from every patient, proliferate indefinitely, and give rise to all cells constituting the bone tissue (Takahashi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Engineering human bone grafts with new macroporous calcium phosphate cement scaffolds

Sladkova

Palmer

Öhman

et al. 2017

J Tissue Eng Regen Med

Self Cite

View full text Add to dashboard Cite

Bone engineering opens the possibility to grow large amounts of tissue products by combining patient-specific cells with compliant biomaterials. Decellularized tissue matrices represent suitable biomaterials, but availability, long processing time, excessive cost, and concerns on pathogen transmission have led to the development of biomimetic synthetic alternatives. We recently fabricated calcium phosphate cement (CPC) scaffolds with variable macroporosity using a facile synthesis method with minimal manufacturing steps and demonstrated long-term biocompatibility in vitro. However, there is no knowledge on the potential use of these scaffolds for bone engineering and whether the porosity of the scaffolds affects osteogenic differentiation and tissue formation in vitro. In this study, we explored the bone engineering potential of CPC scaffolds with two different macroporosities using human mesenchymal progenitors derived from induced pluripotent stem cells (iPSC-MP) or isolated from bone marrow (BMSC). Biomimetic decellularized bone scaffolds were used as reference material in all experiments. The results demonstrate that, irrespective of their macroporosity, the CPC scaffolds tested in this study support attachment, viability, and growth of iPSC-MP and BMSC cells similarly to decellularized bone. Importantly, the tested materials sustained differentiation of the cells as evidenced by increased expression of osteogenic markers and formation of a mineralized tissue. In conclusion, the results of this study suggest that the CPC scaffolds fabricated using our method are suitable to engineer bone grafts from different cell sources and could lead to the development of safe and more affordable tissue grafts for reconstructive dentistry and orthopaedics and in vitro models for basic and applied research.

show abstract

Section: Discussionmentioning

confidence: 69%

Seeding efficiency = {\frac{Number of seeded cells - Number of unattached cells}{Number of seeded cells}}^{*} 100 %

…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Engineering human bone grafts with new macroporous calcium phosphate cement scaffolds

Sladkova

Palmer

Öhman

et al. 2017

J Tissue Eng Regen Med

Self Cite

View full text Add to dashboard Cite

show abstract

Productivity: Cells

Rezaie¹,

Esnaashary²,

Karfarma³

et al. 2020

Bone Cement

View full text Add to dashboard Cite

Joint academic and industrial efforts towards innovative and efficient solutions for clinical needs

Pieri

Ribeiro

Tsiapalis

et al. 2018

J Mater Sci: Mater Med

View full text Add to dashboard Cite

The 4 Translational Research Symposium (TRS) was organised at the annual meeting of the European Society for Biomaterials (ESB) 2017, Athens, Greece, with a focus on 'Academia-Industry Clusters of Research for Innovation Catalysis'. Collaborations between research institutes and industry can be sustained in several ways such as: European Union (EU) funded consortiums; syndicates of academic institutes, clinicians and industries; funding from national governments; and private collaborations between universities and companies. Invited speakers from industry and research institutions presented examples of these collaborations in the translation of research ideas or concepts into marketable products. The aim of the present article is to summarize the key messages conveyed during these lectures. In particular, emphasis is put on the challenges to appropriately identify and select unmet clinical needs and their translation by ultimately implementing innovative and efficient solutions achieved through joint academic and industrial efforts.

show abstract

Fabrication of macroporous cement scaffolds using PEG particles: In vitro evaluation with induced pluripotent stem cell-derived mesenchymal progenitors

Cited by 16 publications

References 61 publications

Engineering human bone grafts with new macroporous calcium phosphate cement scaffolds

Engineering human bone grafts with new macroporous calcium phosphate cement scaffolds

Productivity: Cells

Joint academic and industrial efforts towards innovative and efficient solutions for clinical needs

Contact Info

Product

Resources

About