Fabrication of microspheres and characterization of antimicrobial and anti-inflammatory activity isolated fraction from total alcoholic extract of Cassia Fistula (Linn.) in carrageenan-Type-IV induced inflammatory rats

Abstract

The Cassia fistula Linn is known as the golden shower tree. It’s also referred to as a "disease killer" in Ayurvedic medicine. The current study investigates the effect and estimate of antimicrobial and Anti-inflammatory activity guided fraction isolated from alcoholic extract of Cassia fistula (Linn.) root on carrageenan-Type IV induced Inflammatory rats for using Column fraction of alcoholic extract of root of Cassia fistula (Linn.) An in vivo carrageenan-induced paw edoema model was used to confirm further the anti-inflammatory effect of the fraction aloe-emodin and physcion-loaded microspheres. This study was designed first time for the isolation of alcoholic fraction of aloe emodin and Physcion by root extract and screening of pharmacological activities of Cassia fistula. Aloe-emodin and Physcion fraction demonstrated antibacterial and antifungal effectiveness against the studied pathogens in a dose-dependent manner, according to anti-microbial activity. 50 mg/ml concentration of Physcion-containing microspheres and aloe emodin's antibacterial efficacy against tested pathogens. The anti-inflammatory activity of Aloe emodin and Physcion was found in an equal manner. Both Microsphere samples were found capable of controlling the inflammation till 6 hours. According to the findings of this research, the chosen plant extracts have an immediate anti-inflammatory effect. There are three stages during which carrageenan-induced inflammation mediators are released.

Methanol extract of Isodon ternifolius (D. Don) KUDO leaves has antimicrobial and antioxidant activities but no neuroprotective activity.

“…The exogenous intake of antioxidants as a dietary supplement can decrease oxidative damage (Bouayed and Bohn, 2010;Zhu et al, 2023). Besides plants' flavonoids, phenolics and other phytoconstituents have been reported to exert numerous biological activities, including anti-tumour, antiinflammatory, and antioxidant actions (Gauri et al, 2016;Ullah et al, 2020;Bagchi, 2021;Ghosh et al, 2022;Acharya et al, 2022a, b;Bee et al, 2023;Jakkana and Yamala, 2024). Hence, there is a growing exploration for natural, economic and more effective antioxidant compounds derived from medicinal plants, such as phenolic acids, polyphenols, and flavonoids, than synthetic drugs (Sacchetti et al, 2005;Maiti et al, 2010Maiti et al, , 2013Biswas et al, 2016;Nasim et al, 2022;Hore and Bhaben, 2023;Dey-Ray et al, 2024).…”