Fabrication of multilayer systems combining microfluidic and microoptical elements for fluorescence detection

Roulet, J.-C.; Völkel, R.; Herzig, Hans Peter; Verpoorte, Elisabeth; Rooij, N. F. de; Dändliker, R.

doi:10.1109/84.967369

Cited by 65 publications

(38 citation statements)

References 30 publications

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“…Because these systems often require separation (5), mixing (6,7), and reaction (8) of components with distinct optical profiles, they would benefit from a method that allows the components to be characterized optically in space and time. Advances in miniaturization of components used in spectrophotometric systems have produced a number of useful microsystems: these systems typically work at a single wavelength at any one time (9,10) or perform measurements at a single spatial location (11)(12)(13)(14)(15), and most cannot be easily interfaced with microfluidic systems (16)(17)(18). Miniaturized systems for integrating microspectrometers and microfluidics have been proposed but not demonstrated (19,20).…”

mentioning

confidence: 99%

Space- and time-resolved spectrophotometry in microsystems

Damean

Sia

Linder

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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This work describes a simple optical method for obtaining, in a single still-capture image, the continuous absorbance spectra of samples at multiple locations of microsystems. This technique uses an unmodified bright-field microscope, an array of microlenses, and a diffraction grating to disperse the light transmitted by samples of 10-to 500-m dimensions. By analyzing in a single image the first-order diffracted light, it is possible to collect the full and continuous absorbance spectra of samples at multiple locations (to a spatial resolution of Ϸ8 m) in microwells and microchannels to examine dynamic chemical events (to a time resolution of <10 ms). This article also discusses the optical basis of this method. The simultaneous resolution of wavelength, time, and space at a scale <10 m provides additional capabilities for chemical and biological analysis.poly(dimethylsiloxane) ͉ array of microlenses ͉ spectrophotometer ͉ image processing T his article describes an optical method that resolves wavelength, time, and location simultaneously for samples in microsystems. This technique, which we call micropattern spectrophotometry (PS), analyzes a continuous spectrum of wavelengths (with best performance, in the system described here, in the range of 450 to 700 nm) at multiple positions in the field of view of a microscope. This procedure provides a flexible method for analyzing the composition of samples at a number of points, or in a number of samples, simultaneously and continuously.Microsystems are now ubiquitous in chemistry and biology (1). Applications include analysis of chemical reactions (2), sorting of cells (3), and high-throughput screening (4). Because these systems often require separation (5), mixing (6, 7), and reaction (8) of components with distinct optical profiles, they would benefit from a method that allows the components to be characterized optically in space and time. Advances in miniaturization of components used in spectrophotometric systems have produced a number of useful microsystems: these systems typically work at a single wavelength at any one time (9, 10) or perform measurements at a single spatial location (11-15), and most cannot be easily interfaced with microfluidic systems (16-18). Miniaturized systems for integrating microspectrometers and microfluidics have been proposed but not demonstrated (19,20). Although fiber optic-based microspectrophotometers (21) have been described and microscope-based spectrophotometers (22) are available, they are capable of analyzing only one or only a few samples at a time, and they scan the spectrum one wavelength at a time. Also, they require alignment of a fiber optic cable to the sample region.The method described here collects spectral information at many wavelengths and for many samples simultaneously. Materials and MethodsSetup of the Micropattern Spectrophotometer. We fabricated an array of microlenses in an opaque background by reflowing photoresist (with an index of refraction of 1.59) followed by electroplating of nickel around the mi...

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mentioning

confidence: 99%

Space- and time-resolved spectrophotometry in microsystems

Damean

Sia

Linder

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…In the present study, a simple fluidic device was developed only for the purpose of demonstrating the mi-cromirror's trapping and fluorescence light collection possibilities. More sophisticated devices combining microoptics and microfluidics have been described [33]. Micromirrors may very simply be integrated in similar systems, and are potentially inexpensive and mass producible, e.g.…”

Section: Discussionmentioning

confidence: 99%

Miniaturized high-NA focusing-mirror multiple optical tweezers

Merenda¹,

Rohner²,

Fournier³

et al. 2007

Opt. Express

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An array of high numerical aperture parabolic micromirrors (NA = 0.96) is used to generate multiple optical tweezers and to trap micron-sized dielectric particles in three dimensions within a fluidic device. The array of micromirrors allows generating arbitrarily large numbers of 3D traps, since the whole trapping area is not restricted by the field-of-view of the high-NA microscope objectives used in traditional tweezers arrangements. Trapping efficiencies of Q max r 0.22, comparable to those of conventional tweezers, have been measured. Moreover, individual fluorescence light from all the trapped particles can be collected simultaneously with the high-NA of the micromirrors. This is demonstrated experimentally by capturing more than 100 fluorescent micro-beads in a fluidic environment. Micromirrors may easily be integrated in microfluidic devices, offering a simple and very efficient solution for miniaturized optical traps in lab-on-a-chip devices.

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“…Therefore, their intensities are relatively weaker than when conventional methods are employed. Accordingly, Roulet et al [16] proposed the use of microlenses to focus the light source beam and the fluorescence light, thereby enhancing the detection performance of the microdevice. Similarly, Camou et al [17] reported a 2-D optical lens realized on a poly(dimethylsiloxane) (PDMS) substrate.…”

Section: Introductionmentioning

confidence: 99%

A microfabricated capillary electrophoresis chip with multiple buried optical fibers and microfocusing lens for multiwavelength detection

2005

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We present a new microfluidic device utilizing multiwavelength detection for high-throughput capillary electrophoresis (CE). In general, different fluorescent dyes are only excited by light sources with appropriate wavelengths. When excited by an appropriate light source, a fluorescent dye emits specific fluorescence signals of a longer wavelength. This study designs and fabricates plastic micro-CE chips capable of performing multiple-wavelength fluorescence detection by means of multimode optic fiber pairs embedded downstream of the separation channel. For detection purposes, the fluorescence signals are enhanced by positioning microfocusing lens structures at the outlets of the excitation fibers and the inlets of the detection fibers, respectively. The proposed device is capable of detecting multiple samples labeled with different kinds of fluorescent dyes in the same channel in a single run. The experimental results demonstrate that various proteins, including bovine serum albumin and beta-casein, can be successfully injected and detected by coupling two light sources of different wavelengths to the two excitation optic fibers. Furthermore, the proposed device also provides the ability to measure the speed of the samples traveling in the microchannel. The developed multiwavelength micro-CE chip could have significant potential for the analysis of DNA and protein samples.

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Fabrication of multilayer systems combining microfluidic and microoptical elements for fluorescence detection

Abstract: Abstract-This

Cited by 65 publications

References 30 publications

Space- and time-resolved spectrophotometry in microsystems

Space- and time-resolved spectrophotometry in microsystems

Miniaturized high-NA focusing-mirror multiple optical tweezers

A microfabricated capillary electrophoresis chip with multiple buried optical fibers and microfocusing lens for multiwavelength detection

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