Two natural products; i.e., grapefruit seed extract (GSE) and propolis, were investigated for use in antimicrobial air filters. Staphylococcus aureus was investigated as a test bioaerosol, and was deposited on the antimicrobial filters, which were treated by spraying with various areal densities of GSE and propolis. The pressure drop and particle penetration were investigated to assess the filtration performance of the bioaerosol, and the bacterialinactivation performance of the filters was evaluated by quantifying S. aureus. There was little change in the pressure drop as a function of the areal density of GSE up to 185.9 mg/ cm 2 ; however, a significant change in the pressure drop was found for the air filter coated with propolis at an areal density of 98.4 mg/cm 2 . The penetration levels of bioaerosols in both filters were uniform and in the range 1.4-2.0% (based on particle number), regardless of the areal density of the deposited GSE or propolis. The inactivation rates of the filters with identical deposition masses of GSE and propolis were similar in the ranges of 92. 1-100%, 75.2-89.1%, and 54.4-75.5% at the control filters with colony numbers of 10 3 , 10 4 , and 10 5 CFU/mL, respectively. The bacterial inactivation rate could be described by an exponential function of the areal density of GSE/propolis per number of colonies.