A promising green biosensing material, nanocerium oxide (CeO 2 ) decorated reduced graphene oxide (RGO) nanohybrid reinforced chitosan (CS) nanocomposite has been synthesized as a transducer phase for the effective immobilization of glucose oxidase (GOx) enzyme. CeO 2 nanoparticles were grown on the RGO nanosheet by facile hydrothermal treatment. The effective growth of synthesized nano-CeO 2 on the RGO was evident from X-ray diffraction, scanning electron microscopy and transmission electron microscopy and Raman analysis. Morphological studies confirmed the effective immobilization of GOx on the chitosan nanocomposites modified FTO electrode. The electrocatalytic response of the GOx/nano-CeO 2 -RGO/CS/ FTO bioelectrode was investigated using electrochemical impedance and cyclic voltammetry studies. The obtained results indicate that as compared with bare CeO 2 nanoparticles and RGO, the nano-CeO 2 /RGO nanohybrid shows significant electrochemical activity and provides an adequate microenvironment for effective enzyme immobilization due to the excellent synergism between the CeO 2 nanoparticles and RGO nanosheet. Graphical Abstract Scheme 1: Schematic diagram of graphene nanohybrid reinforced Chitosan nanocomposite modified biosensing matrix.