Fabrication of Novel Silk Fibroin - LDHs Composite Arhitectures for Potential Bone Tissue Engineering

Gălățeanu, Bianca; Radu, I.; Vasile, Eugenia; Hudiţă, Ariana; Serban, Mirela; Costache, Marieta; Iovu, Horia; Zaharia, Cătălin

doi:10.37358/mp.17.4.4921

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…The coating of the basic materials (3 and 4) with sericin and fibroin determined a lower foreign body reaction (inflammatory reaction, peri-implant fibrous capsule), being therefore more biocompatible. This behavior was also characteristic for our previously silk fibroin containing hydrogels [ 18 ]. In another study [ 46 ] silk fibroin was physically deposited onto the surface of a Ti6Al4V biometal to ensure a strong interface between the protein and the metallic implant.…”

Section: Discussionsupporting

confidence: 77%

“…Two major proteins from silk, fibroin and sericin, showed great results for tissue engineering applications [ 16 ]. Fibroin has impressive mechanical properties and a relatively slow degradation rate [ 17 , 18 ]. It can be added to different materials in order to provide more cell recognition sites and promote cell–scaffold interaction [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering

et al. 2022

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Due to their remarkable structures and properties, three-dimensional hydrogels and nanostructured clay particles have been extensively studied and have shown a high potential for tissue engineering as solutions for tissue defects. In this study, four types of 2-hydroxyethyl methacrylate/2-acrylamido-2-methylpropane sulfonic acid/montmorillonite (HEMA/AMPSA/MMT) hydrogels enriched with sericin, and fibroin were prepared and studied in the context of regenerative medicine for soft tissue regenerative medicine. Our aim was to obtain crosslinked hydrogel structures using modified montmorillonite clay as a crosslinking agent. In order to improve the in vitro and in vivo biocompatibility, silk proteins were further incorporated within the hydrogel matrix. Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR) were performed to prove the chemical structures of the modified MMT and nanocomposite hydrogels. Swelling and rheological measurements showed the good elastic behavior of the hydrogels due to this unique network structure in which modified MMT acts as a crosslinking agent. Hydrogel biocompatibility was assessed by MTT, LDH and LIVE/DEAD assays. The hydrogels were evaluated for their potential to support adipogenesis in vitro and human stem cells isolated from adipose tissue were seeded in them and induced to differentiate. The progress was assessed by evaluation of expression of adipogenic markers (ppar-γ2, perilipin) evaluated by qPCR. The potential of the materials to support tissue regeneration was further evaluated on animal models in vivo. All materials proved to be biocompatible, with better results on the 95% HEMA 5% AMPSA enriched with sericin and fibroin material. This composition promoted a better development of adipogenesis compared to the other compositions studied, due the addition of sericin and fibroin. The results were confirmed in vivo as well, with a better progress of soft tissue regeneration after implantation in mice. Therefore, hydrogel 95% HEMA 5% AMPSA enriched with sericin as well as fibroin showed the best results that recommend it for future soft tissue engineering application.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering

et al. 2022

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“…A relatively new concept of polymeric nanocomposite hydrogels has started to overcome these problems by combining the advantage of polymeric hydrogels with the advantage of polymeric nanocomposites [7,8,9,10,11,12,13,14,15]. Nanocomposite hydrogels have been developed by various methods, such as in situ polymerization or pre-modified inorganic nanoparticles [16,17,18,19,20,21]. Modified inorganic nanomaterials have gained special attention, as they can be used as inorganic crosslinkers.…”

Section: Introductionmentioning

confidence: 99%

Novel Nanocomposites Based on Functionalized Magnetic Nanoparticles and Polyacrylamide: Preparation and Complex Characterization

et al. 2019

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This paper reports the synthesis and complex characterization of nanocomposite hydrogels based on polyacrylamide and functionalized magnetite nanoparticles. Magnetic nanoparticles were functionalized with double bonds by 3-trimethoxysilyl propyl methacrylate. Nanocomposite hydrogels were prepared by radical polymerization of acrylamide monomer and double bond modified magnetite nanoparticles. XPS spectra for magnetite and modified magnetite were recorded to evaluate the covalent bonding of silane modifying agent. Swelling measurements in saline solution were performed to evaluate the behavior of these hydrogels having various compositions. Mechanical properties were evaluated by dynamic rheological analysis for elastic modulus and vibrating sample magnetometry was used to investigate the magnetic properties. Morphology, geometrical evaluation (size and shape) of nanostructural characteristics and the crystalline structure of the samples were investigated by SEM, HR-TEM and selected area electron diffraction (SAED). The nanocomposite hydrogels will be further tested for the soft tissue engineering field as repairing scaffolds, due to their mechanical and magnetization behavior that can stimulate tissue regeneration.

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“…The swelling behaviour was investigated applying a traditional procedure [26], and was assessed by calculating mass equilibrium swelling (MES) with equation MES = (Ms-Md)/Md, where Ms is the mass of the hydrogel in swollen state and Md is the mass of the hydrogel in dried state.…”

Section: Equipmentmentioning

confidence: 99%

Ultrasonication - a Potential Method Toward Chitosan Hydrogels

Iftime¹,

Angheloiu²

2019

Mater. Plast.

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The main objective of the paper was to prepare eco-friendly chiral hydrogels from chitosan and betulinic aldehyde under ultrasonic radiation effect, targeting their use for enantiomeric separations. This strategy promoted the obtaining of the hydrogels by supramoleclar organization of the imine units bonding the betuline skeleton with chitosan into ordered clusteres, while not altering the physico-chemical properties of the reagents. FTIR, SEM, CD spectroscopy and POM techniques were used to prove the pelicularities of the hydrogelation mechanism under the ultrasonication. The stability of the hydrogels was investigated by monitoring the influence of the swelling in three media of diferent pH, by POM and CD. It was concluded that the chiral hydrogels prepared by ultrasonication are stable when the pH vary from acidic to basic, indicating the new synthetic approach as a valuable method to yield suitable materials for enatiomeric separations in medical field.

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Fabrication of Novel Silk Fibroin - LDHs Composite Arhitectures for Potential Bone Tissue Engineering

Cited by 8 publications

References 21 publications

Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering

Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering

Novel Nanocomposites Based on Functionalized Magnetic Nanoparticles and Polyacrylamide: Preparation and Complex Characterization

Ultrasonication - a Potential Method Toward Chitosan Hydrogels

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