Fabrication of polymer scaffolds for tissue engineering using surface selective laser sintering

Антонов, Е. Н.; Баграташвили, В. Н.; Howdle, Steven M.; Коновалов, А. Н.; Попов, В. К.; Panchenko, V. Ya.

doi:10.1134/s1054660x06050070

Cited by 34 publications

(13 citation statements)

References 10 publications

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“…Carrier matrices were prepared from polylactide (Poly(D,L)-lactic acid, molecular weight 83 kDa; Alkermes) by the method of SSLS [1]. The powder of polylactide (particle size ~200 μ) was sintered layerby-layer on a SLS-100 selective laser sintering device (Institute for Problems of Laser and Information Technologies).…”

Section: Methodsmentioning

confidence: 99%

“…The proposed method allows us to synthesize matrices with specifi c internal structure from thermally unstable materials (e.g., various bioresorbed polymers). It should be emphasized that the physicochemical properties of materials remain unchanged under these conditions [1].…”

mentioning

confidence: 99%

“…Moreover, this structure provides favorable conditions for the growth of blood vessels and migration of recipient's cells. The composition and strength of the synthetic matrix can vary during SSLS (due to different degree of sintering) [1], which allows us to regulate the time of TEC resorption in tissues [9].…”

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confidence: 99%

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Biocompatibility of Tissue Engineering Constructions from Porous Polylactide Carriers Obtained by the Method of Selective Laser Sintering and Bone Marrow-Derived Multipotent Stromal Cells

et al. 2010

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We studied the biocompatibility of porous polylactide carrier matrices obtained by means of surface selective laser sintering. Carrier matrices had no cytotoxic activity, but maintained adhesion and proliferation of cells. Subcutaneous transplantation of tissue engineering constructions from these carriers and bone marrow-derived multipotent stromal cells did not cause the inflammatory response and pathological changes in rats. The conditions for organotypic regeneration were provided at the site of transplantation (high degree of blood supply and considerable amount of immature precursor cells).

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Biocompatibility of Tissue Engineering Constructions from Porous Polylactide Carriers Obtained by the Method of Selective Laser Sintering and Bone Marrow-Derived Multipotent Stromal Cells

et al. 2010

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“…Different from conventional SLS in which a polymeric material absorbs infrared radiation (e.g. CO 2 laser at λ = 10.6 μm), resulting in a volumetric absorption by the whole polymer particle, SSLS uses near-infrared laser radiation (λ = 0.97 μm) to melt the surface of polymer particles [43]. It is therefore possible to sinter polymer microparticles into solid 3D structures by melting only the near-surface layer of microparticles instead of the whole microparticles.…”

Section: Principle Of Selective Laser Sintering and Modification Of Cmentioning

confidence: 99%

Selective Laser Sintering and Its Biomedical Applications

Duan

Wang

2013

Laser Technology in Biomimetics

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Selective laser sintering (SLS), a mature and versatile rapid prototyping (RP) technology, uses a laser beam to selectively sinter powdered materials to form three-dimensional objects, porous or non-porous, according to the computer-aided design which can be based on data obtained from advanced medical imaging technologies such as magnetic resonance imaging (MRI) and computer tomography (CT). In this chapter, major RP technologies suitable for biomedical applications are briefly introduced first. A review is made on SLS, including its working principle, modification of commercial SLS machines for fabricating biomedical products, biomedical SLS materials, and optimization of SLS parameters. Finally, a detailed presentation is given on the biomedical application of SLS, focusing on the fabrication of tissue engineering scaffolds and drug or biomolecule delivery vehicles. It is shown that SLS has great potential for many biomimetic and biomedical applications.

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“…The details of the processing methodology can be found in refs. [20][21][22]. In brief, powdered polylactide (with the particle size of »200 mm) and nanoscale HA (20%) were mixed and sintered using an SLS-100 selective laser sintering device (Institute of Laser and Information Technologies, Russia).…”

Section: D Scaffoldsmentioning

confidence: 99%

Study of the involvement of allogeneic MSCs in bone formation using the model of transgenic mice

Kuznetsova

Prodanets

Rodimova

et al. 2016

Cell Adhesion & Migration

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Mesenchymal stem cells (MSCs) are thought to be the most attractive type of cells for bone repair. However, much still remains unknown about MSCs and needs to be clarified before this treatment can be widely applied in the clinical practice. The purpose of this study was to establish the involvement of allogeneic MSCs in the bone formation in vivo, using a model of transgenic mice and genetically labeled cells. Polylactide scaffolds with hydroxyapatite obtained by surface selective laser sintering were used. The scaffolds were sterilized and individually seeded with MSCs from the bone marrow of 5-week-old GFP(C) transgenic C57/Bl6 or GFP(¡)C57/Bl6 mice. 4-mm-diameter critical-size defects were created on the calvarial bone of mice using a dental bur. Immediately after the generation of the cranial bone defects, the scaffolds with or without seeded cells were implanted into the injury sites. The cranial bones were harvested at either 6 or 12 weeks after the implantation. GFP(C) transgenic mice having scaffolds with unlabeled MSCs were used for the observation of the host cell migration into the scaffold. GFP(¡) mice having scaffolds with GFP(C) MSCs were used to assess the functioning of the seeded MSCs. The obtained data demonstrated that allogeneic MSCs were found on the scaffolds 6 and 12 weeks post-implantation. By week 12, a newly formed bone tissue from the seeded cells was observed, without an osteogenic predifferentiation. The host cells did not appear, and the control scaffolds without seeded cells remained empty. Besides, a possibility of vessel formation from seeded MSCs was shown, without a preliminary cell cultivation under controlled conditions.

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Fabrication of polymer scaffolds for tissue engineering using surface selective laser sintering

Cited by 34 publications

References 10 publications

Biocompatibility of Tissue Engineering Constructions from Porous Polylactide Carriers Obtained by the Method of Selective Laser Sintering and Bone Marrow-Derived Multipotent Stromal Cells

Biocompatibility of Tissue Engineering Constructions from Porous Polylactide Carriers Obtained by the Method of Selective Laser Sintering and Bone Marrow-Derived Multipotent Stromal Cells

Selective Laser Sintering and Its Biomedical Applications

Study of the involvement of allogeneic MSCs in bone formation using the model of transgenic mice

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