Fabrication of porous chitosan scaffolds for soft tissue engineering using dense gas CO2

Ji, Chao; Annabi, Nasim; Khademhosseini, Ali; Dehghani, Fariba

doi:10.1016/j.actbio.2010.11.043

Cited by 194 publications

(132 citation statements)

References 58 publications

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“…[36,54] The pressures used (corresponding to the total amount of CO 2 initially dissolved), the rate at which the pressures are changed, and the crosslink density of the gel at the time of depressurization can all be metered to gain some level of control over the porosity generated. [55] However, the low to moderate solubility of CO 2 in water is a drawback that limits the resulting porosity.…”

Section: Gas Foamingmentioning

confidence: 99%

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Hoare

2017

Adv Healthcare Materials

178

168

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Structured macroporous hydrogels that have controllable porosities on both the nanoscale and the microscale offer both the swelling and interfacial properties of bulk hydrogels as well as the transport properties of “hard” macroporous materials. While a variety of techniques such as solvent casting, freeze drying, gas foaming, and phase separation have been developed to fabricate structured macroporous hydrogels, the typically weak mechanics and isotropic pore structures achieved as well as the required use of solvent/additives in the preparation process all limit the potential applications of these materials, particularly in biomedical contexts. This review highlights recent developments in the field of structured macroporous hydrogels aiming to increase network strength, create anisotropy and directionality within the networks, and utilize solvent‐free or additive‐free fabrication methods. Such functional materials are well suited for not only biomedical applications like tissue engineering and drug delivery but also selective filtration, environmental sorption, and the physical templating of secondary networks.

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Section: Gas Foamingmentioning

confidence: 99%

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Hoare

2017

Adv Healthcare Materials

178

168

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“…Chitosan is a biocompatible and biodegradable natural polymer that has antibacterial activity and stimulates wound healing with free amine groups in its molecular structure for crosslinking [10]. However, chitosan has low mechanical strength and high degradation rate which limits its application as a matrix for delivery of cells in bone regeneration [11].…”

Section: Introductionmentioning

confidence: 99%

Osteogenic differentiation of human mesenchymal stem cells in freeze-gelled chitosan/nano β-tricalcium phosphate porous scaffolds crosslinked with genipin

Siddiqui

Pramanik

Jabbari

2015

Materials Science and Engineering: C

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The objective of this work was to investigate material properties and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in genipin (GN) crosslinked chitosan/nano β-tricalcium phosphate (CS/nano β-TCP) scaffolds, and compare the results with tripolyphosphate (TPP) crosslinked scaffolds. Porous crosslinked CS/nano β-TCP scaffolds were produced by freeze-gelation using GN (CBG scaffold) and TPP (CBT scaffold) as crosslinkers. The prepared CBT and CBG scaffolds were characterized with respect to porosity, pore size, water content, wettability, compressive strength, mass loss, and osteogenic differentiation of hMSCs. All scaffolds displayed interconnected honeycomb-like microstructures. There was a significant difference between the average pore size, porosity, contact angle, and percent swelling of CBT and CBG scaffolds. The average pore size of CBG scaffolds was higher than CBT, the porosity of CBG was lower than CBT, the water contact angle of CBG was higher than CBT, and the percent swelling of CBG was lower than CBT. At a given crosslinker concentration, there was not a significant difference in compressive modulus and mass loss of CBG and CBT scaffolds. Metabolic activity of hMSCs seeded in CBG scaffolds was slightly higher than CBT. Furthermore, CBG scaffolds displayed slightly higher extent of mineralization after 21 days incubation in osteogenic medium compared to CBT.

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“…When the CS-PVP/BG was cross-linked with genipin, the peak associated with amide I shifts from 1654 to 1647 cm − 1 while the peak arising from amide II shifts from 1567 to 1559 cm − 1 (Fig. 3A(b) and (c)), confirming the reaction between amino groups of CS and ester groups of genipin [26], and formation of hydrogen bonding between CS and genipin [27,28]. Fig.…”

Section: Porosity Of the Scaffold And Cross-linking Degreementioning

confidence: 68%

Multifunctional chitosan/polyvinyl pyrrolidone/45S5 Bioglass® scaffolds for MC3T3-E1 cell stimulation and drug release

Yao

et al. 2015

Materials Science and Engineering: C

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Fabrication of porous chitosan scaffolds for soft tissue engineering using dense gas CO2

Cited by 194 publications

References 58 publications

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Osteogenic differentiation of human mesenchymal stem cells in freeze-gelled chitosan/nano β-tricalcium phosphate porous scaffolds crosslinked with genipin

Multifunctional chitosan/polyvinyl pyrrolidone/45S5 Bioglass® scaffolds for MC3T3-E1 cell stimulation and drug release

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