In this work, porous NiMn2O4 nanosheet arrays on nickel foam (NiMn2O4 NSs@NF) was successfully fabricated by a simple hydrothermal step followed by a heat treatment. Porous NiMn2O4 NSs@NF is directly used as a sensor electrode for electrochemical detecting glucose. The NiMn2O4 nanosheet arrays are uniformly grown and packed on nickel foam to forming sensor electrode. The porous NiMn2O4 NSs@NF electrode not only provides the abundant accessible active sites and the effective ion-transport pathways, but also offers the efficient electron transport pathways for the electrochemical catalytic reaction by the high conductive nickel foam. This synergy effect endows porous NiMn2O4 NSs@NF with excellent electrochemical behaviors for glucose detection. The electrochemical measurements are used to investigate the performances of glucose detection. Porous NiMn2O4 NSs@NF for detecting glucose exhibits the high sensitivity of 12.2 mA mM−1 cm−2 at the window concentrations of 0.99–67.30 μM (correlation coefficient = 0.9982) and 12.3 mA mM−1 cm−2 at the window concentrations of 0.115–0.661 mM (correlation coefficient = 0.9908). In addition, porous NiMn2O4 NSs@NF also exhibits a fast response of 2 s and a low LOD of 0.24 µM. The combination of porous NiMn2O4 nanosheet arrays and nickel foam is a meaningful strategy to fabricate high performance non-enzymatic glucose sensor. These excellent properties reveal its potential application in the clinical detection of glucose.