Fabrication of porous poly(l-lactic acid) scaffolds for bone tissue engineering via precise extrusion

Xiong, Zhuo; Yan, Yongnian; Zhang, Renji; Sun, Lei

doi:10.1016/s1359-6462(01)01094-6

Cited by 119 publications

(56 citation statements)

References 6 publications

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“…The PLGA-␤ -TCP scaffold was produced by lowtemperature deposition manufacturing (LDM) based on the layer-by-layer manufacturing principle of solid freeform fabrication (SFF). Compared with other SFF-based scaffold fabrication processes, such as fused deposition modeling [Hutmacher et al, 2001] and precise extrusion manufacturing [Xiong et al, 2001], the LDM process can better preserve bioactivities of scaffold materials because of its nonheating liquefying processes. A similar process was already used to fabricate porous PLGA scaffolds for cartilage repair in our laboratory [Fan et al, 2006].…”

Section: Introductionmentioning

confidence: 99%

Collagen I Gel Can Facilitate Homogenous Bone Formation of Adipose-Derived Stem Cells in PLGA-β-TCP Scaffold

Wei

et al. 2007

Cells Tissues Organs

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Cell-based tissue engineering is thought to be a new therapy for treatment of bone defects and nonunions after trauma and tumor resection. In this study, we explore the in vitro and in vivo osteogenesis of a novel biomimetic construct fabricated by using collagen I gel to suspend rabbit adipose-derived stem cells (rASCs) into a porous poly(lactic-co-glycolic)acid-β-tricalcium phosphate (PLGA-β-TCP) scaffold (rASCs-COL/PLGA-β-TCP). In vitro and in vivo studies of the rASCs-COL/PLGA-β-TCP composite (group A) were carried out compared with the single combination of rASCs and PLGA-β-TCP (rASCs/PLGA-β-TCP; group B), the combination of acellular collagen I gel and PLGA-β-TCP (COL/PLGA-β-TCP; group C), and the PLGA-β-TCP scaffold (group D). Composites of different groups were cultured in vitro for 2 weeks in osteogenic medium and then implanted into the autologous muscular intervals for 8 weeks. After 2 weeks of in vitro culture, alkaline phosphatase activity and extracellular matrix mineralization in group A were significantly higher than in group B (p < 0.01, n = 4). In vivo osteogenesis was evaluated by radiographic and histological analyses. The calcification level was radiographically evident in group A, whereas no apparent calcification was observed in groups B, C and D (n = 4). In group A, woven bone with a trabecular structure was formed, while in group B, only osteoid tissue was observed. Meanwhile, the bone-forming area in group A was significantly higher than in group B (p < 0.01, n = 4). No bone formation was observed in groups C or D (n = 4). In conclusion, by using collagen I gel to suspend rASCs into porous PLGA-β-TCP scaffold, osteogenic differentiation of rASCs can be improved and homogeneous bone tissue can be successfully formed in vivo.

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Section: Introductionmentioning

confidence: 99%

Collagen I Gel Can Facilitate Homogenous Bone Formation of Adipose-Derived Stem Cells in PLGA-β-TCP Scaffold

Wei

et al. 2007

Cells Tissues Organs

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“…Nozzle-based printing can be categorized into two groups: techniques with melting process and techniques without melting process. Nozzle-based printing with melting process include fused deposition modelling (FDM), precise extrusion manufacturing (PEM), multiphase jet solidification (MJS), precision extrusion deposition (PED), and 3D fiber deposition [31][32][33][34] . FDM technique is the most commonly used nozzle based 3D printing method in which thermoplastic filaments such as poly(lactice acid) (PLA) and acrylonitrile-butadienestyrene (ABS) are melted by heating and extruded on the build plate [35] .…”

Section: Nozzle-based Hydrogel 3d Printing Systemsmentioning

confidence: 99%

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

Jang¹,

Jung²,

Pan³

et al. 2024

IJB

193

117

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Three-dimensional (3D) printing of hydrogels is now an attractive area of research due to its capability to fabricate intricate, complex and highly customizable scaffold structures that can support cell adhesion and promote cell infiltration for tissue engineering. However, pure hydrogels alone lack the necessary mechanical stability and are too easily degraded to be used as printing ink. To overcome this problem, significant progress has been made in the 3D printing of hydrogel composites with improved mechanical performance and biofunctionality. Herein, we provide a brief overview of existing hydrogel composite 3D printing techniques including laser based-3D printing, nozzle based-3D printing, and inkjet printer based-3D printing systems. Based on the type of additives, we will discuss four main hydrogel composite systems in this review: polymer-or hydrogel-hydrogel composites, particle-reinforced hydrogel composites, fiber-reinforced hydrogel composites, and anisotropic filler-reinforced hydrogel composites. Additionally, several emerging potential applications of hydrogel composites in the field of tissue engineering and their accompanying challenges are discussed in parallel.

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“…de Santis et al (2011;2015a;2015b) preformed a series of studies and fabricated nanocomposite magnetic PCL scaffolds (the ratio of PCL and Fe 3 O 4 was 90:10 (w/w)) and novel PCL/FeHA scaffolds by 3D fiber deposition, which confirmed the feasibility of this technology for bone tissue engineering and the advantages of magnetized scaffolds. Xiong et al (2001) developed a PEM system and fabricated a porous PLLA scaffold using this technique. Morphologies of this scaffold are suitable for vascularization and bone tissue regeneration, and it possesses the appropriate mechanical properties.…”

Section: Fused Deposition Modelingmentioning

confidence: 99%

Rapid prototyping technology and its application in bone tissue engineering

Yuan

Zhou

Chen

2017

J. Zhejiang Univ. Sci. B

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Bone defects arising from a variety of reasons cannot be treated effectively without bone tissue reconstruction. Autografts and allografts have been used in clinical application for some time, but they have disadvantages. With the inherent drawback in the precision and reproducibility of conventional scaffold fabrication techniques, the results of bone surgery may not be ideal. This is despite the introduction of bone tissue engineering which provides a powerful approach for bone repair. Rapid prototyping technologies have emerged as an alternative and have been widely used in bone tissue engineering, enhancing bone tissue regeneration in terms of mechanical strength, pore geometry, and bioactive factors, and overcoming some of the disadvantages of conventional technologies. This review focuses on the basic principles and characteristics of various fabrication technologies, such as stereolithography, selective laser sintering, and fused deposition modeling, and reviews the application of rapid prototyping techniques to scaffolds for bone tissue engineering. In the near future, the use of scaffolds for bone tissue engineering prepared by rapid prototyping technology might be an effective therapeutic strategy for bone defects.

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Fabrication of porous poly(l-lactic acid) scaffolds for bone tissue engineering via precise extrusion

Cited by 119 publications

References 6 publications

Collagen I Gel Can Facilitate Homogenous Bone Formation of Adipose-Derived Stem Cells in PLGA-β-TCP Scaffold

Collagen I Gel Can Facilitate Homogenous Bone Formation of Adipose-Derived Stem Cells in PLGA-β-TCP Scaffold

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

Rapid prototyping technology and its application in bone tissue engineering

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