Fabrication of Progesterone-Loaded Nanofibers for the Drug Delivery Applications in Bovine

Abstract

Progesterone is a potent drug for synchronization of the estrus and ovulation cycles in bovine. At present, the estrus cycle of bovine is controlled by the insertion of progesterone-embedded silicone bands. The disadvantage of nondegradable polymer inserts is to require for disposal of these bands after their use. The study currently focuses on preparation of biodegradable progesterone-incorporated nanofiber for estrus synchronization. Three different concentrations (1.2, 1.9, and 2.5 g) of progesterone-impregnated nanofibers were fabricated using electrospinning. The spun membrane were characterized by scanning electron microscopy, X-ray diffraction, differential scanning calorimetry, thermogravimetric analysis, and Fourier transform infrared spectroscopy. Uniform surface morphology, narrow size distribution, and interaction between progesterone and zein were confirmed by SEM. FTIR spectroscopy indicated miscibility and interaction between zein and progesterone. X-ray analysis indicated that the size of zein crystallites increased with progesterone content in nanofibers. Significant differences in thermal behavior of progesterone-impregnated nanofiber were observed by DSC. Cell viability studies of progesterone-loaded nanofiber were examined using MTT assay. In vitro release experiment is to identify the suitable progesterone concentration for estrus synchronization. This study confirms that progesterone-impregnated nanofibers are an ideal vehicle for progesterone delivery for estrus synchronization of bovines.

Development of zein nanofibers for the controlled delivery of essential amino acids for fish nutrition

“…The average diameters ranged from 676 ± 116 nm for zein nanofibers to about 740 ± 117 nm for zein/tryptophan nanofibers. The increase in the average nanofiber diameter due to active agents addition is similar to results found in the literature [17][18][19] and can be interpreted as a success in the incorporation of an essential amino acid (tryptophan) in the zein matrix.…”

Electrospun progesterone‐loaded cellulose acetate nanofibers and their drug sustained‐release profiles

“…[6] Hence, an adequate DDS is imperative to ensure a specific release profile that achieves the desired effect. [7] Nanomaterials have great potential to be applied as drugdelivery agents [8] ; greater loading efficiency can be achieved with their high surface areas, they can be easily manufactured, and their surface can be promptly functionalized with therapeutic agents. [2] Nanomaterialbased drug carriers like nanoparticles, [9] nanocapsules, nanoemulsion, and nanofibers [10] have been extensively studied, [3,5,[11][12][13] and the nanosized DDS can be developed to load a wide range of molecules benefiting the controlled release of various drugs with reduced toxicity, enabling targeted therapy and hindering early inactivation.…”

Direct Metal‐Free Preparation of Functionalizable Polylactic Acid‐Ethisterone Conjugates in a One‐Pot Approach

“…In order to expand the biological application of steroids drug controlled‐release systems, the biological property of PLA‐ES system was further tested preliminarily. The antibacterial efficacy of PLA‐ES conjugate against E. coli was measured by an antibiogram assay .…”

“…As a series of steroids with good bioactivities, estrogens have been exploited in the controlled‐release systems, such as microspheres (especially using PLAs material as carrier), nano‐fibers, gels . Most of them are prepared by solvent volatilization, impregnation, electrostatic spinning, and other physical technologies to improve continuous and sustained releasing performance of drug and its encapsulation rate.…”