Fabrication of Redox-Controllable Bioinspired Nanochannels for Precisely Regulating Protein Transport

Tu, Le; Sheng, Qin; Li, Yuntao; Chen, Xiaoya; Han, Yunfeng; Li, Junrong; Xiong, Xiaoxing; Sun, Yao; Li, Haibing

doi:10.1021/acsami.2c05594

Cited by 2 publications

(2 citation statements)

References 60 publications

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“…Protein transport through nanopores and nanochannels is also highly relevant in other applications beyond sensing. Micrometer-long nanochannels were chemically modified to prepare high-flux protein transporters with potential applications in biomolecular delivery. , Enzyme confinement in silica nanopores resulted in biocatalytic materials. − Polymer nanopores have been used to prepare membranes for the separation of protein mixtures. , …”

Section: Introductionmentioning

confidence: 99%

Orientational Pathways during Protein Translocation through Polymer-Modified Nanopores

Gonzalez Solveyra,

Perez Sirkin,

Tagliazucchi

et al. 2024

ACS Nano

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Protein translocation through nanopores holds significant promise for applications in biotechnology, biomolecular analysis, and medicine. However, the interpretation of signals generated by the translocation of the protein remains challenging. In this way, it is crucial to gain a comprehensive understanding on how macromolecules translocate through a nanopore and to identify what are the critical parameters that govern the process. In this study, we investigate the interplay between protein charge regulation, orientation, and nanopore surface modifications using a theoretical framework that allows us to explicitly take into account the acid−base reactions of the titrable amino acids in the proteins and in the polyelectrolytes grafted to the nanopore surface. Our goal is to thoroughly characterize the translocation process of different proteins (GFP, β-lactoglobulin, lysozyme, and RNase) through nanopores modified with weak polyacids. Our calculations show that the charge regulation mechanism exerts a profound effect on the translocation process. The pH-dependent interactions between proteins and charged polymers within the nanopore lead to diverse free energy landscapes with barriers, wells, and flat regions dictating translocation efficiency. Comparison of different proteins allows us to identify the significance of protein isoelectric point, size, and morphology in the translocation behavior. Taking advantage of these insights, we propose pHresponsive nanopores that can load proteins at one pH and release them at another, offering opportunities for controlled protein delivery, separation, and sensing applications.

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Section: Introductionmentioning

confidence: 99%

Orientational Pathways during Protein Translocation through Polymer-Modified Nanopores

Gonzalez Solveyra,

Perez Sirkin,

Tagliazucchi

et al. 2024

ACS Nano

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“…A biological channel protein, located within the cell membrane, is a smart ion channel that reacts to environmental stimuli and controls the transfer of substances, information, and energy across cellular and subcellular structures, which is essential for the fundamental functions of all living organisms. − Based on the design of biological channel proteins, a variety of solid-state nanochannels have been created to control the transport of ions because of the ability to adjust size at the nanoscale, versatile chemical properties, and strong mechanical capacity. − As a result, they have the potential to be used as smart nanofluidic devices for applications such as biosensing, − DNA/protein sequencing, − selective permeability, − and water-energy nexus. − …”

Section: Introductionmentioning

confidence: 99%

Stimuli-Responsive Ion Transport Regulation in Nanochannels by Adhesion-Induced Functionalization of Macroscopic Outer Surface

Jiang,

Wang,

et al. 2024

ACS Appl. Mater. Interfaces

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Responsive regulation of ion transport through nanochannels is crucial in the design of smart nanofluidic devices for sequencing, sensing, and water-energy nexus. Functionalization of the inner wall of the nanochannel enhances interaction with ions and fluid but restricts versatile chemical approaches and accurate characterizations of fluidic interfaces. Herein, we reveal a responsive regulating mechanism of ion transport through nanochannels by polydopamine (PDA)-induced functionalization on the macroscopic outer surface of nanochannels. Responsive molecules were codeposited with PDA on the outer surface of nanochannels and formed a valve of nanometer thickness to manually manipulate ion transport by changing its gap spacing, surface charge, and wettability under external stimulus. The response ratio can be up to 100fold by maximizing the proportion of responsive molecules on the outer surface. Laminating the codepositions of different responsive molecules with PDA on the channel's outer surface produces multiple responses. A nearly universal adhesion of PDA with responsive molecules on the open outer surface induces nanochannels responsive to different external stimuli with variable response ratios and arbitrary combinations. The results challenge the primary role of functionalization on the nanoconfined interface of nanofluidics and open opportunities for developing new-style nanofluidic devices through the functionalization of macroscopic interface.

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Fabrication of Redox-Controllable Bioinspired Nanochannels for Precisely Regulating Protein Transport

Cited by 2 publications

References 60 publications

Orientational Pathways during Protein Translocation through Polymer-Modified Nanopores

Orientational Pathways during Protein Translocation through Polymer-Modified Nanopores

Stimuli-Responsive Ion Transport Regulation in Nanochannels by Adhesion-Induced Functionalization of Macroscopic Outer Surface

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