Fabrication of resveratrol coated gold nanoparticles and investigation of their effect on diabetic retinopathy in streptozotocin induced diabetic rats

Dong, Yi; Wan, Guangming; Yan, Panshi; Cheng, Qian; Li, Fuzhen; Peng, Guang-Hua

doi:10.1016/j.jphotobiol.2019.04.012

Cited by 78 publications

(72 citation statements)

References 18 publications

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“…It has been shown that gallic and protocatechuic acids possess reducing abilities in forming nanoparticles [39-42]. Hesperidin [43,44], diosmin, and naringin [44], curcumin [45], guavanoic acid [46], phloridzin, an antidiabetic agent found in fruits and its aglycon [47], escin [48], resveratrol [49], and gymnemic acid [50] were found to be responsible for the biosynthesis of Au NPs. Other compounds that have been used in the formation of Au NPs with antidiabetic properties include chitosan, chondroitin sulfate, tyrosine and tryptophan [51][52][53].Like the metal-reducing ability of plant extracts, the antioxidants activity has been associated with the plants' phenolics [54].…”

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confidence: 99%

Green Synthesis of Gold Nanoparticles Capped with Procyanidins from Leucosidea sericea as Potential Antidiabetic and Antioxidant Agents

et al. 2020

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In this study, procyanidins fractions of dimers and trimers (F1-F2) from the Leucosidea sericea total extract (LSTE) were investigated for their chemical constituents. The total extract and the procyanidins were employed in the synthesis of gold nanoparticles (Au NPs) and fully characterized. Au NPs of 6, 24 and 21 nm were obtained using LSTE, F1 and F2 respectively. Zeta potential and in vitro stability studies confirmed the stability of the particles. The enzymatic activity of LSTE, F1, F2 and their corresponding Au NPs showed strong inhibitory alpha-amylase activity where F1 Au NPs demonstrated the highest with IC 50 of 1.88 µg/mL. On the other hand, F2 Au NPs displayed the strongest alpha-glucosidase activity at 4.5 µg/mL. F2 and F2 Au NPs also demonstrated the highest antioxidant activity, 1834.0 ± 4.7 µM AAE/g and 1521.9 ± 3.0 µM TE/g respectively. The study revealed not only the ability of procyanidins dimers (F1 and F2) in forming biostable and bioactive Au NPs but also, a significant enhancement of the natural products activities, which could improve the smart delivery in future biomedical applications.Biomolecules 2020, 10, 452 2 of 24 applying nanoparticles through the green route is their biocompatibility [5]. Many green synthesized nanoparticles have demonstrated interesting biological activities [5,10] including antimicrobial and antidiabetic properties.Diabetes mellitus has undoubtedly become a serious health challenge. More worrisome is type II diabetes mellitus (T2DM), which accounts for 90% of diabetes mellitus. It occurs as a result of the inefficient processing of insulin [11]. As of 2017, the population of adults between the ages of 20 and 79 that suffered from diabetes was 425 million [12]. This is equivalent to 9.9% of the world's population [12]. By estimation, this disease would have drastically increased by 48% in 28 years if not properly managed [13]. Although drugs like miglitol, vigliobose as well as acarbose are available in the market, they are costly and their continuous use is associated with side effects like diarrhea, dropsy, heart failure, damage to the liver, weight gain, abdominal pain, hyperglycemia, and flatulence, necessitating the need for more potent and newer remedies [14][15][16].It is well established that bioactive compounds in various plants possess significant effects in delaying and management of T2DM [17]. Extracts from different plants have been reported as alpha-glucosidase and alpha-amylase inhibitors [18][19][20][21][22][23][24][25][26][27]. Additionally, biologically synthesized Au NPs using plant extracts showed interesting antidiabetic activity. The extracts of Chamalcostus cuspidatus [28], Gymnema sylvestre [29], Cassia fistula stem bark [30], Hericium erinaceus [31], Turbinaria conoides [32], Sambucus nigra [33] and Sargassum swartzi [34] displayed antidiabetic activities in various investigations. Silver/gold NPs of Ocimum basilicum [35] and cinnamon extract [36] also lowers glucose levels. The use of single molecules as reducing/stabilizing agents for ...

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Green Synthesis of Gold Nanoparticles Capped with Procyanidins from Leucosidea sericea as Potential Antidiabetic and Antioxidant Agents

et al. 2020

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“…The obvious observation is that when the diabetes-induced rats were evaluated for their retinal vascular permeability after treatment with RES-AuNPs, the permeability got reduced, and the blood vessels remained normal. Also, the thickness of the retina of the normal group animals was sizably thick, and the retinae became thinner on treatment with RES-AuNPs [149].…”

Section: Effect Of Res and Gold Nanoparticles On Diabetic Retinopathymentioning

confidence: 95%

“…Resveratrol, with its anti-oxidative properties and which acts as an anti-inflammatory agent, has been encapsulated in gold nanoparticles (AuNP) [149] and tested for its efficacy [148] in reducing the effects of diabetic retinopathy. The prepared nanoparticles were 10 nm in size and had a crystalline structure.…”

Section: Effect Of Res and Gold Nanoparticles On Diabetic Retinopathymentioning

confidence: 99%

“…Subsequently, the levels of Vascular Cell Adhesion Molecule 1 (VCAM-1), Intercellular Adhesion Molecule-1 (ICAM-1) and Monocyte Chemoattractant Protein-1 (MCP-1) also increased due to diabetes-induced changes and the conditions returned to normal after treatment with AuNPs. The increase in inflammatory cytokines and chemokines occurs through the phosphorylation of the extracellular signal-regulated kinase (ERK) 1/2 and NF-KP phosphorylation, and it appeared to be higher in the diabetes-induced groups which were reduced significantly using AuNP when compared to the normal group [149].…”

Section: Effect Of Res and Gold Nanoparticles On Diabetic Retinopathymentioning

confidence: 99%

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Resveratrol Nanoparticles: A Promising Therapeutic Advancement over Native Resveratrol

et al. 2020

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The importance of fruit-derived resveratrol (RES) in the treatment of various diseases has been discussed in various research publications. Those research findings have indicated the ability of the molecule as therapeutic in the context of in vitro and in vivo conditions. Mostly, the application of RES in in vivo conditions, encapsulation processes have been carried out using various nanoparticles that are made of biocompatible biomaterials, which are easily digested or metabolized, and RES is absorbed effectively. These biomaterials are non-toxic and are safe to be used as components in the biotherapeutics. They are made from naturally available by-products of food materials like zein or corn or components of the physiological system as with lipids. The versatility of the RES nanoparticles in their different materials, working range sizes, specificity in their targeting in various human diseases, and the mechanisms associated with them are discussed in this review.

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“…AuNPs were shown to inhibit VEGF-induced migration in HUVEC [14] and laser-induced CNV in mice [15]. Fabrication of resveratrol-coated gold nanoparticles can increase the retinal pigment epithelium-derived factor and decrease the VEGF-1 in streptozotocin-induced diabetic rats [16]. However, the effects of AuNPs on the choroid-retina endothelial (RF/6A) cells viability and VEGF-induced RF/6A cell migration are still unknown.…”

Section: Introductionmentioning

confidence: 99%

The Inhibitory Effects of Gold Nanoparticles on VEGF-A-Induced Cell Migration in Choroid-Retina Endothelial Cells

Chan

Hsiao

et al. 2019

IJMS

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Background: Vascular endothelial growth factor (VEGF) is upregulated by hypoxia and is a crucial stimulator for choroidal neovascularization (CNV) in age-related macular degeneration and pathologic myopia, as well as retinal neovascularization in proliferative diabetic retinopathy. Retinal and choroidal endothelial cells play key roles in the development of retinal and CNV, and subsequent fibrosis. At present, the effects of gold nanoparticles (AuNPs) on the VEGF-induced choroid-retina endothelial (RF/6A) cells are still unknown. In our study, we investigated the effects of AuNPs on RF/6A cell viabilities and cell adhesion to fibronectin, a major ECM protein of fibrovascular membrane. Furthermore, the inhibitory effects of AuNPs on RF/6A cell migration induced by VEGF and its signaling were studied. Methods: The cell viability assay was used to determine the viability of cells treated with AuNPs. The migration of RF/6A cells was assessed by the Transwell migration assay. The cell adhesion to fibronectin was examined by an adhesion assay. The VEGF-induced signaling pathways were determined by western blotting. Results: The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay revealed no cytotoxicity of AuNPs on RF/6A cells. AuNPs inhibited VEGF-induced RF/6A cell migration in a concentration-dependent manner but showed no significant effects on RF/6A cell adhesion to fibronectin. Inhibitory effects of AuNPs on VEGF-induced Akt/eNOS were found. Conclusions: These results suggest that AuNPs are an effective inhibitor of VEGF-induced RF/6A cell migration through the Akt/eNOS pathways, but they have no effects on their cell viabilities and cell adhesion to fibronectin.

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Fabrication of resveratrol coated gold nanoparticles and investigation of their effect on diabetic retinopathy in streptozotocin induced diabetic rats

Cited by 78 publications

References 18 publications

Green Synthesis of Gold Nanoparticles Capped with Procyanidins from Leucosidea sericea as Potential Antidiabetic and Antioxidant Agents

Green Synthesis of Gold Nanoparticles Capped with Procyanidins from Leucosidea sericea as Potential Antidiabetic and Antioxidant Agents

Resveratrol Nanoparticles: A Promising Therapeutic Advancement over Native Resveratrol

The Inhibitory Effects of Gold Nanoparticles on VEGF-A-Induced Cell Migration in Choroid-Retina Endothelial Cells

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