Fabrication of Scaffolds for Bone-Tissue Regeneration

Chocholata, Petra; Kulda, Vlastimil; Babuška, Václav

doi:10.3390/ma12040568

Cited by 470 publications

(354 citation statements)

References 136 publications

(262 reference statements)

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“…Biocompatible scaffolds employed in tissue engineering, comprising a variety of natural, synthetic, conductive polymers, and elastic polymer networks such as hydrogels [6][7][8], combined with signaling molecules and/or growth factors [9][10][11][12]. In addition to polymers, scaffolds were further fabricated from bioceramics, bioactive glasses, and their composites [12][13][14][15][16]. In the same context, decellularization was introduced as a novel scaffold fabrication technique that depends on maintaining the extracellular matrix with its organization, architecture, and vascular network, thus obtaining a cell-free 3D structure harboring biological signals, affecting the cell behavior and differentiation [17].…”

Section: Introductionmentioning

confidence: 99%

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

Moshy

Radwan

Rady

et al. 2020

Stem Cells International

108

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Regenerative medicine literature has proposed mesenchymal stem/progenitor cell- (MSC-) mediated therapeutic approaches for their great potential in managing various diseases and tissue defects. Dental MSCs represent promising alternatives to nondental MSCs, owing to their ease of harvesting with minimally invasive procedures. Their mechanism of action has been attributed to their cell-to-cell contacts as well as to the paracrine effect of their secreted factors, namely, secretome. In this context, dental MSC-derived secretome/conditioned medium could represent a unique cell-free regenerative and therapeutic approach, with fascinating advantages over parent cells. This article reviews the application of different populations of dental MSC secretome/conditioned medium in in vitro and in vivo animal models, highlights their significant implementation in treating different tissue’ diseases, and clarifies the significant bioactive molecules involved in their regenerative potential. The analysis of these recent studies clearly indicate that dental MSCs’ secretome/conditioned medium could be effective in treating neural injuries, for dental tissue regeneration, in repairing bone defects, and in managing cardiovascular diseases, diabetes mellitus, hepatic regeneration, and skin injuries, through regulating anti-inflammatory, antiapoptotic, angiogenic, osteogenic, and neurogenic mediators.

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Section: Introductionmentioning

confidence: 99%

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

Moshy

Radwan

Rady

et al. 2020

Stem Cells International

108

View full text Add to dashboard Cite

show abstract

“…with the active groups from collagen belonging to the bone matrix, thus promoting an intimate connection to the implant (fig 2). The bone matrix is composed of organic components, such as elastic collagen fibers, as well as inorganic mineral components such as hydroxyapatite and calcium phosphate [20]. Collagen provides resistance to fracture due to its elasticity [21,22] and inorganic mineralization ensures bone rigidity [23].…”

Section: Resultsmentioning

confidence: 99%

Assessment of the Mechanical Properties of Orthopedic Screws Coated with Polyurethane Acrylate Containing Hydroxyapatite, Intended to Fix the Fragility Fractures

Filip¹,

Alexa²,

Sîrbu³

et al. 2019

Mat.Plast.

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The fragility fracture fixation confronts with the major problem of implant loosening due to the altered bone structure. Techniques used to fragility fracture stabilization includes metals devices, cements or adhesives. Different types of cements and adhesive can be obtained by chemical manipulation in order to provide a more efficient transition between the metal surface and the real bone. Thus, by selecting the appropriate chemical composition and ration between the components, synthetic cement and adhesive can provide a proper interface that ensure a perfect cohesion between the implant material and the natural bone. Most of the studies point the benefit of these synthetic materials in improving screw fixation strength. That is why, currently, the synthetic materials used in prosthesis are improved by associating with natural components of the bone, such as hydroxyapatite. For osteoporosis, which is characterized by demineralization, the association of the implanted material with hydroxyapatite is expected to be a suited solution for bone matrix regeneration after implantation. The aim of the current study was to assess the mechanical properties of orthopedic screws coated with a new polyurethane acrylate polymer containing hydroxyapatite in order to improve the stability of the screw for the subsequent fixation of the fragility fracture. To test the efficiency of the new hydroxyapatite containing polymer, the mechanical behavior of the coated screws was evaluated. Our data show that the augmented screw can be obtained by incorporating lower hydroxyapatite concentrations.

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“…Alternatively, diverse fractions of flakes, powders and nano forms of chitin (i.e., nanowhiskers, nanofibrils and nanocrystals) [128][129][130] can be obtained from demineralized and deproteinated crustaceans' chitin. Such scaffolding strategies [131], however, are connected with technological difficulties and other disadvantages which can be a critical weakness in terms of cost and future clinical use [132].…”

Section: Discussionmentioning

confidence: 99%

3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo

et al. 2020

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Structure-based tissue engineering requires large-scale 3D cell/tissue manufacture technologies, to produce biologically active scaffolds. Special attention is currently paid to naturally pre-designed scaffolds found in skeletons of marine sponges, which represent a renewable resource of biomaterials. Here, an innovative approach to the production of mineralized scaffolds of natural origin is proposed. For the first time, a method to obtain calcium carbonate deposition ex vivo, using living mollusks hemolymph and a marine-sponge-derived template, is specifically described. For this purpose, the marine sponge Aplysin aarcheri and the terrestrial snail Cornu aspersum were selected as appropriate 3D chitinous scaffold and as hemolymph donor, respectively. The formation of calcium-based phase on the surface of chitinous matrix after its immersion into hemolymph was confirmed by Alizarin Red staining. A direct role of mollusks hemocytes is proposed in the creation of fine-tuned microenvironment necessary for calcification ex vivo. The X-ray diffraction pattern of the sample showed a high CaCO3 amorphous content. Raman spectroscopy evidenced also a crystalline component, with spectra corresponding to biogenic calcite. This study resulted in the development of a new biomimetic product based on ex vivo synthetized ACC and calcite tightly bound to the surface of 3D sponge chitin structure.

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Fabrication of Scaffolds for Bone-Tissue Regeneration

Cited by 470 publications

References 136 publications

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

Dental Stem Cell-Derived Secretome/Conditioned Medium: The Future for Regenerative Therapeutic Applications

Assessment of the Mechanical Properties of Orthopedic Screws Coated with Polyurethane Acrylate Containing Hydroxyapatite, Intended to Fix the Fragility Fractures

3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo

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