Fabrication of Scratched Nanogrooves for Highly Oriented Cell Alignment and Application as a Wound Healing Dressing

Abstract

Using improper wound care materials may cause impaired wound healing, which can involve scar formation and infection. Herein, we propose a facile method to fabricate a cellalignment scaffold, which can effectively enhance cell growth and migration, leading to the reproduction of cellular arrangements and restoration of tissues. The principle is scratching a diamond lapping film that gives uniaxial nanotopography on substrates. Cells are seeded to follow the geometric cue via contact guidance, resulting in highly oriented cell alignment. Remarkable biocompatibility is also demonstrated by the high cell viability on various substrates. In vivo studies in a wound healing model in mice show that the scratched film supports directed cell guidance on the nanostructure, with significantly reduced wound areas and inhibition of excessive collagen deposition. Rapid recovery of the epidermis and dermis is also shown by histological analyses, suggesting the potential application of the scratching technique as an advanced wound dressing material for effective tissue regeneration.

Natural vs Synthetic Polymers: How Do They Communicate with Cells for Skin Regeneration—A Review

“…Due to their versatility, PCL-based biocomposites can be fabricated into different shapes and sizes, which can mimic the properties of extracellular matrix (ECM) but can also activate the fibroblast growth factors and support the cellular migration, adhesion, proliferation, and angiogenesis processes. Several composite polymers based on PCL have been employed to date, aiming to repair skin tissues including core-shell electrospun nanofibers [137], conductive biomimetic bilayer fibrous scaffold [138], hydrogels [139], 3D-printed dressings [140], nanocomposite sponges [141], scratched nano grooves films [142], air-jet spinning film [143], injectable thermosensitive hydrogel [144], and decellularized-extracellular-matrix-decorated PolyHIPE scaffolds [145].…”

Silk Nanofiber Scaffolds with Multiple Angiogenic Cues to Accelerate Wound Regeneration

“…Skin wounds are common due to burns, traffic accidents, tumors, and diabetes . Wound dressings are widely used to protect wounds and favor skin regeneration. − Synthetic and natural biopolymers have been fabricated to form skin grafts with niches supportive of wound healing. − Chemical signals (e.g., growth factors, exosomes, inhibitors, bioactive drugs) and physical cues (e.g., stiffness, nanomicromorphologies, aligned topography) influence cell behavior and have been introduced to wound dressings to accelerate wound healing. − However, the challenge remains to optimize multiple cues simultaneously in these types of materials in order to optimize skin regeneration.…”

Balanced chemical reactivity, antimicrobial properties and biocompatibility of decellularized dermal matrices for wound healing

“…1,2 This, in turn, facilitates the infiltration of harmful microorganisms, leading to infections that impede wound healing and may even result in scarring. 3,4 Therefore, when the skin is damaged by trauma, surgery or disease, issues such as prevention of bacterial infection and rapid wound repair become particularly important, and this has been a key concern of medical clinics in recent years. 5,6 Wound healing has been greatly influenced by skin tissue engineering techniques recently, with porcine acellular dermal matrix (pADM) now being the most representative wound dressing.…”