Fabrication of Sensitive Potentiometric Cholesterol Biosensor Based on Co<sub>3</sub>O<sub>4</sub> Interconnected Nanowires

Ibupoto, Zafar Hussain; Elhag, Sami; Nur, Omer; Willander, Magnus

doi:10.1002/elan.201400192

Cited by 11 publications

(5 citation statements)

References 49 publications

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“…However, the electrochemical sensors incorporating a biological component can overcome these pitfalls . Thus, electrochemical biosensors are the most lucrative choice for cholesterol detection and quantification , though there might be issues of poor enzyme immobilization and ineffective integration with non‐biocompatible nano‐materials. It increases the risk of partial denaturation of a redox‐active prosthetic part in the enzyme, the facilitator for direct electron transfer .…”

Section: Introductionmentioning

confidence: 99%

One Step Electrode Fabrication for Direct Electron Transfer Cholesterol Biosensor Based on Composite of Polypyrrole, Green Reduced Graphene Oxide and Cholesterol Oxidase

2018

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A simple single‐step fabrication of a novel biocomposite electrode was used for cholesterol sensing through direct electron transfer. The bio‐composite was prepared by polypyrrole, green reduced graphene oxide (gRGO) and cholesterol oxidase (ChOx). The graphene oxide (GO) was “green” reduced, with the Paederia foetide (PFL) plant extract at 60 °C to improve its biocompatibility. The adherence of gRGO to the electrode surface was improved through its co‐deposition during electro‐polymerization of pyrrole. Interestingly, the enzyme ChOx also could be immobilized during this co‐deposition process, resulting in one step biosensor fabrication. The optimization of electrochemical deposition parameters on glassy carbon electrode for this enzymatic bioelectrode (ChOx‐gRGO‐PPy/GCE) has resulted in excellent sensitivity (1095.3 μA mM−1 cm−2), wide linear range (0.01–6 mM), and low detection limit (3.78 μM) of cholesterol. Also, the Dubinin‐Radushkevitch (D−R) adsorption study revealed the occurrence of physisorption (ϵ=3.35) process in this bioelectrode, confirming diffusion‐controlled reaction. Reproducibility of electrodeposition procedure, impeccable selectivity in the presence of interferrants, repeatability for multiple use and long‐term stability for practical use were also ensured. This could be used to construct the cost‐effective device for cholesterol diagnosis.

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Section: Introductionmentioning

confidence: 99%

One Step Electrode Fabrication for Direct Electron Transfer Cholesterol Biosensor Based on Composite of Polypyrrole, Green Reduced Graphene Oxide and Cholesterol Oxidase

2018

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“…For example in some metal oxides semiconductors palladium or platinum were predominantly the dopants used for enhancing gas sensing [ 16 ]. The same principle can be applied to biosensors [ 17 ]. On the other hand, abnormal levels of cholesterol are related to be the cause of atherosclerosis and heart diseases [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Co3O4 Cotton-Like Nanostructures for Cholesterol Biosensor

et al. 2014

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The use of templates to assist and possess a control over the synthesis of nanomaterials has been an attractive option to achieve this goal. Here we have used sodium dodecyl sulfate (SDS) to act as a template for the low temperature synthesis of cobalt oxide (Co3O4) nanostructures. The use of SDS has led to tune the morphology, and the product was in the form of “cotton-like” nanostructures instead of connected nanowires. Moreover, the variation of the amount of the SDS used was found to affect the charge transfer process in the Co3O4. Using Co3O4 synthesized using the SDS for sensing of cholesterol was investigated. The use of the Co3O4 synthesized using the SDS was found to yield an improved cholesterol biosensor compared to Co3O4 synthesized without the SDS. The improvement of the cholesterol sensing properties upon using the SDS as a template was manifested in increasing the sensitivity and the dynamic range of detection. The results achieved in this study indicate the potential of using template assisted synthesis of nanomaterials in improving some properties, e.g., cholesterol sensing.

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“…Other nanostructured forms of ZnO, such as NTs [409][410][411][412], NWs [413-417], nanosheets [418], nanowalls [419], nanoflakes [420], nanotetrapods [421], and nanoflowers [422] were directly grown on an electrode surface for the fabrication of Au electrode using hydrothermal methods [429,430]. These synthesized nanomaterials as an ideal electrode to detect glucose [431].…”

Section: Laser Scribingmentioning

confidence: 99%

Deposition of nanomaterials: A crucial step in biosensor fabrication

Ahmad

Wolfbeis

Hahn

et al. 2018

Materials Today Communications

178

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 An easy, stable, and reproducible nanomaterial deposition/growth method is crucial for the realization of fabricated biosensor devices' performance, such as device stability, and reproducibility, as well as other sensing properties.  Key challenges for optimum deposition include stability, reproducibility, repeatability, and the ability of deposited nanomaterials to address these challenges is critiqued.

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Fabrication of Sensitive Potentiometric Cholesterol Biosensor Based on Co₃O₄ Interconnected Nanowires

Cited by 11 publications

References 49 publications

One Step Electrode Fabrication for Direct Electron Transfer Cholesterol Biosensor Based on Composite of Polypyrrole, Green Reduced Graphene Oxide and Cholesterol Oxidase

One Step Electrode Fabrication for Direct Electron Transfer Cholesterol Biosensor Based on Composite of Polypyrrole, Green Reduced Graphene Oxide and Cholesterol Oxidase

Synthesis of Co3O4 Cotton-Like Nanostructures for Cholesterol Biosensor

Deposition of nanomaterials: A crucial step in biosensor fabrication

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Fabrication of Sensitive Potentiometric Cholesterol Biosensor Based on Co3O4 Interconnected Nanowires

Cited by 11 publications

References 49 publications

One Step Electrode Fabrication for Direct Electron Transfer Cholesterol Biosensor Based on Composite of Polypyrrole, Green Reduced Graphene Oxide and Cholesterol Oxidase

One Step Electrode Fabrication for Direct Electron Transfer Cholesterol Biosensor Based on Composite of Polypyrrole, Green Reduced Graphene Oxide and Cholesterol Oxidase

Synthesis of Co3O4 Cotton-Like Nanostructures for Cholesterol Biosensor

Deposition of nanomaterials: A crucial step in biosensor fabrication

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Fabrication of Sensitive Potentiometric Cholesterol Biosensor Based on Co₃O₄ Interconnected Nanowires