Fabrication of Sericin/Agrose Gel Loaded Lysozyme and Its Potential in Wound Dressing Application

Abstract

Sericin is a biomaterial resource for its significant biodegradability, biocompatibility, hydrophilicity, and reactivity. Designing a material with superabsorbent, antiseptic, and non-cytotoxic wound dressing properties is advantageous to reduce wound infection and promote wound healing. Herein, we propose an environment-friendly strategy to obtain an interpenetrating polymer network gel through blending sericin and agarose and freeze-drying. The physicochemical characterizations of the sericin/agarose gel including morphology, porosity, swelling behavior, crystallinity, secondary structure, and thermal property were well characterized. Subsequently, the lysozyme loaded sericin/agarose composite gel was successfully prepared by the solution impregnation method. To evaluate the potential of the lysozyme loaded sericin/agarose gel in wound dressing application, we analyzed the lysozyme loading and release, antimicrobial activity, and cytocompatibility of the resulting gel. The results showed the lysozyme loaded composite gel had high porosity, excellent water absorption property, and good antimicrobial activities against Escherichia coli and Staphylococcus aureus. Also, the lysozyme loaded gel showed excellent cytocompatibility on NIH3T3 and HEK293 cells. So, the lysozyme loaded sericin/agarose gel is a potential alternative biomaterial for wound dressing.

Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application

“…To evaluate the cytotoxicity of SS/Agar, PDA-SS/Agar and AgNPs-PDA-SS/Agar films, cell counting kit-8 (CCK-8) assay was performed to examine the cells treated with different films. In the test, the metabolically active cells react with the tetrazolium salt in the CCK-8 solution to produce a soluble formaldehyde nitrogen dye with maximum absorbance at 450 nm [ 39 ]. Optical density (OD) reflects cell survival and living cells [ 40 ].…”

Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications

“…It can form strong gels at low concentrations [ 7 , 8 , 9 ]. Considering its malleable mechanical properties, low cost, and good biocompatibility, agarose is often used to improve the performance of sericin as an auxiliary biomaterial [ 9 , 10 , 11 , 12 ].…”

Does sericin pleurodesis increase the basic fibroblastic growth factor and high-sensitive c-reactive protein levels in rat plasma?

“…Sericin has good hydrophilic properties that are widely used in the pharmaceuticals and cosmetic sectors. Furthermore, it is useful in the wound healing process and can be used as a wound dressing and healing agent [16][17][18]. Ersel et al evaluated the in vivo wound healing effects of a gel formulation containing sericin in rats [16].…”