Fabrication of Silver Nanoparticles Against Fungal Pathogens

Mansoor, Sheikh; Zahoor, Irfana; Baba, Tawseef Rehman; Padder, Shahid Ahmad; Bhat, Zulfiqar Ali; Koul, Aabid; Jiang, Lijing

doi:10.3389/fnano.2021.679358

Cited by 101 publications

(59 citation statements)

References 127 publications

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“…The AgNPs fabricated using Kleinia grandiflora leaf extract at the concentration of 200 µg/mL showed considerable antimicrobial activity against Pseudomonas aeruginosa and Aspergillus niger [ 59 ]. Furthermore, Mansoor, et al [ 60 ] reported that AgNPs inhibited the growth of various fungi, including Aspergillus fumigates, Aspergillus niger, Aspergillus flavus, Trichophyton rubrum, Candida albicans, and Penicillium species.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Green Silver Nanoparticles Fabricated by Spirulina platensis Phycocyanin as Anticancer and Antimicrobial Agents

Soror

Ahmed

Alharbi

et al. 2022

Life

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Green nanotechnology has attracted attention worldwide, especially in treating cancer and drug-resistant section 6 microbes. This work aims to investigate the anticancer activity of green silver nanoparticles synthesized by Spirulina platensis phycocyanin (SPAgNPs) on two cancer cell lines: Lung cancer cell line (A-549) and breast cancer cell line (MCF-7), compared to the normal human lung cell line (A138). We also aimed to investigate the bactericidal activity against Staphylococcus aureus ATCC29737, Bacillus cereus ATCC11778, Escherichia coli ATCC8379, and Klebsiella pneumonia, as well as the fungicidal activity against Candida albicans (ATCC6019) and Aspergillus niger. The obtained SPAgNPs were spherical and crystalline with a size of 30 nm and a net charge of −26.32 mV. Furthermore, they were surrounded by active groups responsible for stability. The SPAgNPs scavenged 85% of the DPPH radical with a relative increase of approximately 30% over the extract. The proliferation of cancer cells using the MTT assay clarified that both cancer cells (A-549 and MCF-7) are regularly inhibited as they grow on different concentrations of SPAgNPs. The maximum inhibitory effect of SPAgNPs (50 ppm) reached 90.99 and 89.51% against A-549 and MCF7, respectively. Regarding antimicrobial activity, no inhibition zones occurred in bacterial or fungal strains at low concentrations of SPAgNPs and the aqueous Spirulina platensis extract. However, at high concentrations, inhibition zones, especially SPAgNPs, were more potent for all tested microorganisms than their positive controls, with particular reference to Staphylococcus aureus, since the inhibition zones were 3.2, 3.8, and 4.3 mm, and Bacillus cereus was 2.37 mm when compared to tetracycline (2.33 mm). SPAgNPs have more potent antifungal activity, especially against Aspergillus niger, compared to their positive controls. We concluded that SPAgNPs are powerful agents against oxidative stress and microbial infection.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Green Silver Nanoparticles Fabricated by Spirulina platensis Phycocyanin as Anticancer and Antimicrobial Agents

Soror

Ahmed

Alharbi

et al. 2022

Life

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“…In plant pathology, Ag-NPs demonstrated antifungal and antiviral activity, as well as considerable nematicidal activity [ 28 , 29 ], improving plant growth indices and fruit output [ 30 ]. The application of Ag-NPs improved plant tolerance to a variety of biotic stressors, including phytopathogenic fungi [ 26 , 31 ] and worms [ 19 , 28 ]. Their application to plant viral diseases, however, is relatively limited [ 17 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Ocimum basilicum-Mediated Synthesis of Silver Nanoparticles Induces Innate Immune Responses against Cucumber Mosaic Virus in Squash

Abdelkhalek

El‐Gendi

Al-Otibi

et al. 2022

Plants

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Cucumber mosaic virus (CMV) causes a significant threat to crop output sustainability and human nutrition worldwide, since it is one of the most prevalent plant viruses infecting most kinds of plants. Nowadays, different types of nanomaterials are applied as a control agent against different phytopathogens. However, their effects against viral infections are still limited. In the current study, the antiviral activities of the biosynthesized silver nanoparticles (Ag-NPs) mediated by aqueous extract of Ocimum basilicum against cucumber mosaic virus in squash (Cucurbita pepo L.) were investigated. The prepared Ag-NPs were characterized using scanning electron microscopy (SEM), dynamic light scattering (DLS), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR) and zeta potential distribution techniques. DLS, SEM, and TEM analyses showed that the Ag-NPs were spherical, with sizes ranging from 26.3 to 83 nm with an average particle size of about 32.6 nm. FTIR identified different functional groups responsible for the capping and stability of Ag-NPs. The zeta potential was reported as being −11.1 mV. Under greenhouse conditions, foliar sprays of Ag-NPs (100 µg/mL) promoted growth, delayed disease symptom development, and significantly reduced CMV accumulation levels of treated plants compared to non-treated plants. Treatment with Ag-NPs 24 h before or after CMV infection reduced CMV accumulation levels by 92% and 86%, respectively. There was also a significant increase in total soluble carbohydrates, free radical scavenging activity, antioxidant enzymes (PPO, SOD, and POX), as well as total phenolic and flavonoid content. Furthermore, systemic resistance was induced by significantly increasing the expression levels of pathogenesis-related genes (PR-1 and PR-5) and polyphenolic pathway genes (HCT and CHI). These findings suggest that Ag-NPs produced by O. basilicum could be used as an elicitor agent and as a control agent in the induction and management of plant viral infections.

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“…Nanoparticles may be classified into organic and inorganic varieties [3][4][5][6]. Throughout the globe, inorganic nanoparticles have been used in nanomedicine, medication delivery, cosmetics, electronics, and the energy sector [7,8]. Among metal nanoparticles, silver nanoparticles (Ag NPs) have received attention not only in the researches field.…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis and Characterization of Silver Nanoparticles / CM-Chitosan-Isopropanol by Gamma Irradiations Method

Beden¹,

Ati²,

Alimarah³

et al. 2022

RCMA

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The present study demonstrates a green process for the synthesis of spherical-shaped and stabilized silver nanoparticles Ag NPs using gamma irradiated conditions. This method has certain advantages over conventional methods since it can manage the particle size and structure and generates totally reduced and extremely pure nanoparticles free from by-products or chemical reducing agents. Silver nanoparticles composite (Ag NPs / CM) is prepared from an aqueous solution of silver nitrate, chitosan and isopropanol, at room temperature using gamma irradiation doses to induce reduction and cross-linking to formation in situ Ag NPs/ CM-chitosan-isopropanol solutions. The production and homogenous distribution of silver nanoparticles in the hydrogel matrix were characterized using transmission electron microscopy, XRD, Zeta potential and UV-Vis spectrophotometer analysis. The silver/ CM-chitosan-isopropanol matrixes possessed different gamma doses using a Co- 60 gamma source. Through the comprehensive results of IR- Ag NPs /(CM- chitosan isopropanol solutions due to antibacterial activity test, the results show the prepared Ag NPs /CM-chitosan-isopropanol could be used as an antibacterial agent. The obtained Ag nanoparticles were stable for over 3 months at room temperature. the analysis outcomes indicate that the colloidal Solution (silver nanoparticles / (CM chitosan - isopropanol)) has a promising ability to be used in antibacterial applications in the future.

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Fabrication of Silver Nanoparticles Against Fungal Pathogens

Cited by 101 publications

References 127 publications

Evaluation of Green Silver Nanoparticles Fabricated by Spirulina platensis Phycocyanin as Anticancer and Antimicrobial Agents

Evaluation of Green Silver Nanoparticles Fabricated by Spirulina platensis Phycocyanin as Anticancer and Antimicrobial Agents

Ocimum basilicum-Mediated Synthesis of Silver Nanoparticles Induces Innate Immune Responses against Cucumber Mosaic Virus in Squash

Green Synthesis and Characterization of Silver Nanoparticles / CM-Chitosan-Isopropanol by Gamma Irradiations Method

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