Fabrication of silver nanoparticles from leaf extract of Butea monosperma (Flame of Forest) and their inhibitory effect on bloom-forming cyanobacteria

Chaturvedi, Venkatesh; Verma, Pradeep

doi:10.1186/s40643-015-0048-6

Cited by 22 publications

(9 citation statements)

References 31 publications

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“…The diff erences in size, charge, stability, shape, and morphology of AgNPs can be characterized by UVvisible spectra. The peak in UV-visible spectra of the synthesized AgNPs had a maximum at 414 nm similar to that reported for AgNPs synthesized by Butea monosperma (36), while, had a blue shift compared to that measured for AgNPs synthesized by Cycas leaf (37). The diff erence is due to the size and shape of the AgNPs.…”

Section: Antifungal Activity Of Agnpssupporting

confidence: 76%

Biosynthesis of Silver Nanoparticles Using Pine Pollen and Evaluation of the Antifungal Efficiency

Khatami

Mortazavi

Kishani-Farahani

et al. 2017

Iran. J. Biotechnol.

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Nanoparticles have been applied to medicine, hygiene, pharmacy and dentistry, and will bring significant advances in the prevention, diagnosis, drug delivery and treatment of disease. Green synthesis of metal nanoparticles has a very important role in nanobiotechnology, allowing production of non-toxic and eco-friendly particles. Green synthesis of silver nanoparticles (AgNPs) was studied using pine pollen as a novel, cost-effective, simple and non-hazardous bioresource. The antifungal activity of the synthesized AgNPs was investigated. Biosynthesis of AgNPs was conducted using pollen of pine (as a novel bioresource) acting as both reducing and capping agents. AgNPs were characterized using UV-visible spectroscopy, X-ray diffraction and transmission electron microscopy. In evaluation for antifungal properties, the synthesized AgNPs represented significant in vitro inhibitory effects on cultures. Pine pollen can mediate biosynthesis of colloidal AgNPs with an average size of 12 nm. AgNPs were formed at 22 °C and observed to be highly stable up to three months without precipitation or decreased antifungal property. AgNPs showed significant inhibitory effects against. The first report for a low-cost, simple, well feasible and eco-friendly procedure for biosynthesis of AgNPs was presented. The synthesized AgNPs by pine pollen were nontoxic and eco-friendly, and can be employed for large-scale production. The nanoparticles showed strong effect on quantitative inhibition and disruption of antifungal growth.

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Section: Antifungal Activity Of Agnpssupporting

confidence: 76%

Biosynthesis of Silver Nanoparticles Using Pine Pollen and Evaluation of the Antifungal Efficiency

Khatami

Mortazavi

Kishani-Farahani

et al. 2017

Iran. J. Biotechnol.

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“…Green chemistry mediated formation of nanoparticle is a kind of "bottom up" approach, where the main reaction is reduction/oxidation. In biologically mediated nanoparticle synthesis, the microbial enzymes or plant phytochemicals having reducing or antioxidant properties are usually responsible for the reduction of metal ions into their respective metal nanoparticles (Keat et al 2015;Nath and Banerjee 2013;Chaturvedi and Verma 2015). Biosynthesis of silver nanoparticles using bacteria, fungi, and plants is already well documented (Durán et al 2011;Chaturvedi and Verma 2015;Ingle et al 2009;Thirunavoukkarasu et al 2013).…”

mentioning

confidence: 99%

“…In biologically mediated nanoparticle synthesis, the microbial enzymes or plant phytochemicals having reducing or antioxidant properties are usually responsible for the reduction of metal ions into their respective metal nanoparticles (Keat et al 2015;Nath and Banerjee 2013;Chaturvedi and Verma 2015). Biosynthesis of silver nanoparticles using bacteria, fungi, and plants is already well documented (Durán et al 2011;Chaturvedi and Verma 2015;Ingle et al 2009;Thirunavoukkarasu et al 2013). In fungal community, various fungal strains have been well reported, as an efficient bio-factory for the synthesis of metal nanoparticles Jaidev and Narasimha 2010;Saravanan and Nanda 2010).…”

mentioning

confidence: 99%

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

Barapatre

Aadil

Jha

2016

Bioresour. Bioprocess.

140

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Background:The fabrication of silver nanoparticles (Ag-NPs) through green chemistry is an emerging area in the field of medical nanotechnology. Ag-NPs were fabricated by enzymatic reduction of AgNO 3 using two lignin-degrading fungus Aspergillus flavus (AfAg-NPs) and Emericella nidulans (EnAg-NPs). The prepared Ag-NPs were characterized by different spectroscopic techniques. Antibacterial activity of prepared Ag-NPs was demonstrated against selected Gram negative (Escherichia coli and Pseudomonas aeruginosa) and Gram positive (Staphylococcus aureus) bacteria in the term of minimum bactericidal concentration (MBC) and susceptibility constant (Z). The synergistic antibacterial activity of Ag-NPs with four conventional antibiotics was also determined by the fractional inhibitory concentration index (FICI) using the checkerboard microdilution method. The antibiofilm potential of Ag-NPs was also tested. Results:The plasmon surface resonance of biosynthesized Ag-NPs shows its characteristic peaks at UV and visible region (~450 and 280 nm). Fourier transform infrared spectrometer (FTIR) analysis confirms the nature of the capping agents as protein (enzyme) and indicates the role of protein (enzyme) in reduction of silver ions. The average particle size and charge of synthesized Ag-NPs was ~100 nm and ~−20 mV, respectively. X-ray diffraction (XRD) and TEM analysis confirmed the purity, shape, and size (quasi-spherical, hexagonal, and triangular) of Ag-NPs. Energy-dispersive X-ray spectroscopy (EDX) data validate the biological synthesis of Ag-NPs. Low MBC and high susceptibility constant indicate the high antimicrobial strength of biosynthesized Ag-NPs. The antibacterial analysis demonstrates the synergistic antimicrobial activity of Ag-NPs with antibiotics. This study also shows that biosynthesized Ag-NPs have ability to inhibit the biofilm formation by 80-90 %. Conclusion:The Aspergillus flavus and Emericella nidulans-mediated biosynthesized Ag-NPs have significant antimicrobial activity and demonstrate synergistic effect in combination with antibiotics. It suggests that nanoparticles can be effectively used in combination with antibiotics to improve the efficacy of antibiotics against pathogenic microbes. The substantial antibiofilm efficiency of biosynthesized Ag-NPs would also be helpful against sensitive and multidrug-resistant strains.

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“…The nanoparticles were centrifuged and dried for further analysis. (10,3) Quantitative assay for biofilm inhibition : Biofilm inhibition assay was carried out in flat bottomed 96-wells microtitre plates. Pseudomonas culture (laboratory isolate )was inoculated in sterile 50ml of King's-B broth in an 100ml Erlenmeyer flask and incubated at 37°C for 24 hours under aerobic condition for the assay.…”

Section: Characterization Of Agnp'smentioning

confidence: 99%

Comparative analysis of biofabricated silver nanoparticles as antibiofilm agents on Pseudomonas aeruginosa

Anju¹,

Sarada²

2020

CTBP

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Microbial cells in the biofilm were characterized with significant features like antimicrobial resistance and bio fouling properties, which make them distinguishable from their planktonic forms. Although many antimicrobial drugs and antibiotics were reported for biofilm inhibition and dispersal, these compounds had limited applications because of drug resistance. Development of antibiofilm agents which could overcome the drug resistant bacteria had been a challenge over the years. In order to overcome development of drug resistance, silver nanoparticles were being used, to coat the medical devices. This strategy, though successful to some extent, suffer with toxic effects on eukaryotic cells in application .Hence there arises a need to explore natural compounds with antibiofilm activity. Natural products established as antibacterial agents were also found to have quorum quenching activity. Garcinia (Garcinia cambogia) and Ginger (Zingiber officinale) extracts were observed to possess quorum quenching activity in the lab, therefore used for biofabrication to enhance the antimicrobial activity of silver nanoparticles. Pseudomonas aeruginosa being a most potent biofilm forming bacteria in various environments, like infections, food spoilage and bio fouling in medical devices etc. The study aimed to figure out a novel antibiofilm agent, which could inhibit or eliminate biofilms by using a combination of biologically synthesized nanoparticles and natural quorum quenching agents. Comparative analysis of bio fabricated nanoparticles on the inhibition and dispersal of Pseudomonas biofilms was performed with non fabricated nanoparticles using microtiter based assay, followed by SEM and Flow cell cytometry techniques. Fabricated GNP (fGNP) had showed 17.9% more inhibition which indicates ~ 2 fold enhancement in biofilm inhibition activity .Similarly 9.45% enhancement was found in dispersal activity by fGNP. These results were encouraging us to improve upon the combinatorial approach and to formulate an ideal antibiofilm agent with minimal scope for emergence of antimicrobial resistance.

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Fabrication of silver nanoparticles from leaf extract of Butea monosperma (Flame of Forest) and their inhibitory effect on bloom-forming cyanobacteria

Cited by 22 publications

References 31 publications

Biosynthesis of Silver Nanoparticles Using Pine Pollen and Evaluation of the Antifungal Efficiency

Biosynthesis of Silver Nanoparticles Using Pine Pollen and Evaluation of the Antifungal Efficiency

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

Comparative analysis of biofabricated silver nanoparticles as antibiofilm agents on Pseudomonas aeruginosa

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