Fabrication of stable antibody-modified field effect transistors using electrical activation of Schiff base cross-linkages for tumor marker detection

Hideshima, Sho; Sato, Ryosuke; Kuroiwa, Shigeki; Osaka, Tetsuya

doi:10.1016/j.bios.2010.10.023

Cited by 55 publications

(33 citation statements)

References 36 publications

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“…However, as shown in Figure 1c, the R q value for the antibody-immobilized surface was greater than that of the Fab-immobilized surface. In line with our previous reports [9], the height of the immobilized antibodies was measured as 4–5 nm. Thus, the Fab molecules were smaller than the antibodies, and were believed to lie flat on the gate surface.…”

Section: Resultssupporting

confidence: 83%

“…Increasing the concentration of AFP increased the amount of negatively charged AFP molecules within the Debye length, resulting in a greater shift of Δ V g . In our previous study [9], the detection limit for AFP of our antibody-immobilized FET was found to be 10 ng/mL. The use of the smaller receptor Fab increased the sensitivity by lowering the detection limit from 10 ng/mL to 100 pg/mL.…”

Section: Resultsmentioning

confidence: 85%

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Field Effect Transistor Biosensor Using Antigen Binding Fragment for Detecting Tumor Marker in Human Serum

et al. 2014

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Detection of tumor markers is important for cancer diagnosis. Field-effect transistors (FETs) are a promising method for the label-free detection of trace amounts of biomolecules. However, detection of electrically charged proteins using antibody-immobilized FETs is limited by ionic screening by the large probe molecules adsorbed to the transistor gate surface, reducing sensor responsiveness. Here, we investigated the effect of probe molecule size on the detection of a tumor marker, α-fetoprotein (AFP) using a FET biosensor. We demonstrated that the small receptor antigen binding fragment (Fab), immobilized on a sensing surface as small as 2–3 nm, offers a higher degree of sensitivity and a wider concentration range (100 pg/mL–1 μg/mL) for the FET detection of AFP in buffer solution, compared to the whole antibody. Therefore, the use of a small Fab probe molecule instead of a whole antibody is shown to be effective for improving the sensitivity of AFP detection in FET biosensors. Furthermore, we also demonstrated that a Fab-immobilized FET subjected to a blocking treatment, to avoid non-specific interactions, could sensitively and selectively detect AFP in human serum.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 85%

Field Effect Transistor Biosensor Using Antigen Binding Fragment for Detecting Tumor Marker in Human Serum

et al. 2014

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“…Thus, the activity of target analyte results in an additional chemical contribution on the threshold voltage (Vth) [5]. The main applications of EGFETs are associated with the detection of ionic species, pH and specific molecules (through the functionalization of sensitive surfaces) such as urea and glucose [5][6][7][8][9][10]. Apart from its prevalent use in the field of biosensors, physical sensors for high frequency ultrasound detection (e.g.…”

Section: Unlikementioning

confidence: 99%

EGFET Based Sensors for Bioanalytical Applications: A Review

Pullano¹,

Critello²,

Mahbub³

et al. 2018

Preprint

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Since 1970s, a great deal of attention has been paid to the development of semiconductor–based biosensors because of the numerous advantages they offer, including high sensitivity, faster response time, miniaturization, and low–cost manufacturing for quick biospecific analysis with reusable features. Commercial biosensors have become highly desirable in the fields of medicine, food, environmental monitoring as well as military applications (e.g., Hoffmann–La Roche, Abbott Point of Care, Orion High technologies, etc.), whereas increasing concerns on the food safety and health issues have resulted in the introduction of novel legislative standards for these sensors. Numerous devices have been developed for monitoring of biological–processes such as nucleic–acid hybridization, protein–protein interaction, antigen–antibody bonds and substrate–enzyme reactions, just to name a few. Since 1980s scientific interest moved to the development of semiconductor–based devices which also include integrated front–end electronics, such as the extended–gate–field–effect–transistor biosensor which is one of the first miniaturized chemical sensors. This work is intended to be a review of the state of the art focused on the development of biosensors based extended–gate–field–effect–transistor within the field of bioanalytical applications, which will highlight the most recent research works reported in the literature. Moreover, a comparison among the diverse EGFET devices will be presented giving particular attention to the materials and technologies.

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“…Recently, biosensors have been proposed as promising analytical devices and employed in different fields, such as clinical diagnostics, both with or without labeling [ 13 ]. Label-free biosensors based on silicon nanowire field-effect transistors (SiNW-FETs) have been successful applied in cancer diagnosis with excellent specificity and high sensitivity [ 14 ]. SiNW-FET biosensors, consisting of source, drain and gate electrodes, detect the changes of charge density on the surface of the SiNW caused by the intrinsic charge of adsorbed biomolecules [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of α-Fetoprotein and Carcinoembryonic Antigen Based on Si Nanowire Field-Effect Transistors

Zhu

Zhang

et al. 2015

Sensors

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Primary hepatic carcinoma (PHC) is one of the most common malignancies worldwide, resulting in death within six to 20 months. The survival rate can be improved by effective treatments when diagnosed at an early stage. The α-fetoprotein (AFP) and carcinoembryonic antigen (CEA) have been identified as markers that are expressed at higher levels in PHC patients. In this study, we employed silicon nanowire field-effect transistors (SiNW-FETs) with polydimethylsiloxane (PDMS) microfluidic channels to simultaneously detect AFP and CEA in desalted human serum. Dual-channel PDMS was first utilized for the selective modification of AFP and CEA antibodies on SiNWs, while single-channel PDMS offers faster and more sensitive detection of AFP and CEA in serum. During the SiNW modification process, 0.1% BSA was utilized to minimize nonspecific protein binding from serum. The linear dynamic ranges for the AFP and CEA detection were measured to be 500 fg/mL to 50 ng/mL and 50 fg/mL to 10 ng/mL, respectively. Our work demonstrates the promising potential of fabricated SiNW-FETs as a direct detection kit for multiple tumor markers in serum; therefore, it provides a chance for early stage diagnose and, hence, more effective treatments for PHC patients.

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Fabrication of stable antibody-modified field effect transistors using electrical activation of Schiff base cross-linkages for tumor marker detection

Cited by 55 publications

References 36 publications

Field Effect Transistor Biosensor Using Antigen Binding Fragment for Detecting Tumor Marker in Human Serum

Field Effect Transistor Biosensor Using Antigen Binding Fragment for Detecting Tumor Marker in Human Serum

EGFET Based Sensors for Bioanalytical Applications: A Review

Simultaneous Detection of α-Fetoprotein and Carcinoembryonic Antigen Based on Si Nanowire Field-Effect Transistors

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