Fabrication of Three-Dimensional Scaffolds Based on Nano-biomimetic Collagen Hybrid Constructs for Skin Tissue Engineering

Abstract

Three-dimensional (3D) biodegradable and biomimetic porous scaffolds are ideal frameworks for skin tissue engineering. In this study, hybrid constructs of 3D scaffolds were successfully fabricated by the freeze-drying method from combinations of the type I collagen (Col) and synthetic poly(lactic acid) (PLLA) or polycaprolactone (PCL). Four different groups of 3D porous scaffolds including PCL, PCL–Col, PCL–PLLA, and PCL–PLLA–Col were fabricated and systematically characterized by hydrogen nuclear magnetic resonance, Fourier transform infrared spectroscopy, and scanning electron microscopy (SEM). Adipose tissue-derived mesenchymal stem cells (AT-MSCs) were seeded in all scaffolds, and the viability, proliferation, and adhesion of the cells were investigated using dimethylthiazol diphenyltetrazolium bromide assay and SEM. The results showed that scaffolds containing Col, particularly PCL–PLLA–Col scaffold, with pore sizes close to 400 nm and being sufficiently interconnected, have significantly greater potential ( p < 0.01) for encouraging AT-MSCs adhesion and growth. The PCL–PLLA provided a mechanically stronger mesh support, and the type I Col microsponges encouraged excellent cell adhesion and tissue formation. The scaffold with the best properties could be an appropriate functional candidate for the preparation of artificial skin constructs.

The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male rats

“…Despite the aforementioned functions, the poor water solubility of chrysin and curcumin hinders its application as a proper drug for wound healing [32][33][34]. This limitation calls for the creation of an appropriate conveyor to increase the in vivo stability of the bioactive compound, bioavailability and eliminate their poor water solubility.…”

Construction and Evaluation of Composite Scaffold Properties of Cellulose Base Enhanced with Calcium Alginate Containing Propolis for Use in Skin Tissue Engineering

“…In all samples, porosity is above 70%, Which is one of the requirements for tissue engineering scaffolds [20]. Figure 2 was illustrated FT IR spectrum of Calcium Alginate / CMC / Propolis sample.…”

Electroconductive Nanobiomaterials for Tissue Engineering and Regenerative Medicine

“…Select cellular activity and intracellular signaling can be enhanced due to electrically conductive materials. 4,8 This review article highlights the very recent advancements in the development of different types of electroconductive nanobiomaterials (including nanofibrous scaffolds, hydrogels, hybrid scaffolds, films, and 3D printed constructs) that can recapitulate the electrical and cellular behavior of a specific tissue required for translatable regenerative medicine. Furthermore, an overview of the existing technologies and examples of these scaffolds (with particular emphasis on electroconductive scaffolds) for cardiac, nerve, bone, and skeletal muscle tissue engineering are summarized and discussed.…”

“…From a biomaterial perspective, the engineered cardiac tissues for treating MI are normally produced by seeding heart cells within 3D porous biomaterial scaffolds that mimic the ECM of the native tissue and organs. 23 These biomaterials, which are usually made of either biological polymers (carbohydrates, lipids, proteins, and nucleic acids), include collagen 24 and alginate, 23 or synthetic polymers such as poly(lactic acid) (PLA), 8,25,26 help cells to organize into functioning tissues, but poor conductivity of these materials limits the ability of these scaffolds to contract strongly as a unit. This is mainly because of the porous properties of these engineered myocardial scaffolds, which lead to limited intercellular connection and electrical signal propagation due to isolating pore walls.…”