Fabrication, optimization and application of a dip-probe fluorescence spectrometer based on white-light excitation fluorescence

Abstract

A dip-probe adapter was designed for easy analysis of complex multifluorophoric systems. The newly fabricated fiber optic compatible spectrometer design with dip-probe adapter could reach and analyze fluorophores kept at a distance from the analyzer. The new design and its parameters were optimized using standard fluorophores to get an optimal spectral response. This white-light excitation fluorescence (WLEF) based spectrometer could analyze any fluorophoric system rapidly without changing the instrument configuration. Fuel–oil blends such as petrol–2T oil and petrol–4T oil were precisely quantified with high accuracy (0.3% error) using a WLEF–multivariate analysis combination.

Simultaneous Quantification of Multiple Polycyclic Aromatic Hydrocarbons in Aqueous Media using Micelle Assisted White Light Excitation Fluorescence

“…A fiber optic compatible and portable Dip Probe Fiber Optic Spectrometer 25 was fabricated for the WLEF measurement using a white light excitation source (270-900 nm, W-X lamp); sensitive CCD-Diode Array Detector (DAD, QE9000-Pro) and UV-VIS XRS Solarization Resistant fibers (1 m, transmission range:180-900 nm, diameter: 600 μm and NA: 0.22). The components were purchased from Ocean Optics Inc.…”

Design of a Portable and Reliable Fluorimeter with High Sensitivity for Molecule Trace Analysis

“…Furthermore, the required instrumentation employed for fluorometric measurements has evolved in recent years from static designs mainly focused on laboratory assays towards more flexible, portable and handheld devices [10][11][12][13][14][15][16], which can be easily operated for in situ experiments. Thus, the increasing availability of the necessary components to build fluorometric detection devices, such as intense light sources with different excitation wavelengths (LEDs, fiber optic) [17,18] and compact micro-spectrometers for light analysis (C1280 MA) [19], and the existence of numerous computer-programmable microcontrollers (Raspberry Pi, Arduino) [20][21][22][23] allow the building of low-cost equipment [24][25][26] with unique features for in situ sample analysis with spectrofluorometric detection.…”

Spectrally resolved thermoluminescence measurements in fluorescence spectrometer

“…Nowadays, however, it is compact fluorescent spectrometers and fluorometers with developed accessories that have gained a widespread use in investigating and evaluating the optical emission parameters of the different nature materials [10][11][12][13][14]. Ready-to-use commercial solutions (e.g., the Perkin Elmer Fluorescence Spectrometer [10], Thermo Scientific Lumina [11], Agilent Fluorescence Detector [12], etc.)…”