Background: In early 2020, a global pandemic (COVID-19) broke out and severely affected the progress of education in various countries’ universities and institutions, which promoted the progress of online courses at the same time. This article aims to conduct a comparative analysis of teacher-student surveys between online live teaching and traditional off-line teaching, and explore the direction of medical education reform in colleges and universities.Methods: Surveys among teachers and students were conducted on live broadcast courses and traditional offline courses by Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China. Teachers were investigated from the three aspects including preparation before the class, the experience of teaching during the class, and evaluation after the class, while the students were performed investigations and statistical analysis from the aspects of class experience, learning effect, evaluation of the teachers and curricula.Results: Both teachers and students fully recognized the orderly organization and management of online live-streaming teaching, and that online courses have a positive and powerful role in improving the quality of teaching. However, teachers and students agree that the overall teaching experience and learning effect of online courses are inferior to offline, so the traditional offline courses or the mixation of online and offline modes are tended for teaching.Conclusions: The effective and proper use of online education in stomatology can help improve teaching effect significantly, but it is impossible to replace the traditional classroom. In the future, with the addition of online teaching, stomatological education could adopt a new mode of the combination of online and offline teaching as well as the integration of inside and outside the classroom.