“…Total 16Murrahbuffaloes were selected having normal genitalia and not having any history of dystocia or any genital disease, randomly divided into two groups one for fixed time NM with Murrah bull and other for AI with frozen thawed Murrah bull semen after synchronization of estrus (Figure 2). Expression of estrus was observed onward day 11 during the early morning and late evening for 20-30 min each time after estrus synchronization (Mohan et [33,8,4,23,21] , viz., visual observations: mucus discharge, bellowing (Qureshi et al 1998;Brar and Nanda, 2004; Singh and Barwal, 2010; Ashok, 2013) [45,13,49,5] and Vulvar edema (Danell et al, 1984; Ullah and Usmani, 1985) [18,55] and transrectal palpation (Prasad et al 1986; Singh, 2013; Gamit et al 2015) [44,51,22] . The status of pregnancy was examined by rectal palpation on day 90 post insemination (natural and artificial).…”