Facile and Green Production of Human Milk Fat Substitute through <i>Rhodococcus opacus</i> Fermentation

Zhang, Lin-Shang; Chu, Mei-Yun; Zong, Min‐Hua; Yang, Juan; Lou, Wen‐Yong

doi:10.1021/acs.jafc.0c03185

Cited by 8 publications

(4 citation statements)

References 46 publications

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“…These compounds are used as an alternative food to human breast milk. No cytotoxic effects were observed, supporting the feasibility of using microalgae oils for human nutrition from birth to 2 years of age or more [97][98][99]. Similarly, the composition and use of species such as B. braunii, C. vulgaris, H. pluvialis, and Isochrysis galbana have been studied.…”

Section: Applications In Human Nutritionmentioning

confidence: 75%

“…Based on their high nutritional value, reduced environmental impact, and economic sustainability, microalgae have been used as functional ingredients to enhance the characteristics of foods [101], such as milk or dairy products [99] and meat products [90], or in the manufacturing of snacks [102,103], food supplements [104,105], and food pig-ments [103,106]. This highlights the importance of research, development, and innovation in the use of microalgae for human consumption.…”

Section: Applications In Human Nutritionmentioning

confidence: 99%

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Microalgae: Potential for Bioeconomy in Food Systems

et al. 2021

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The efficient use of natural resources is essential for the planet’s sustainability and ensuring food security. Colombia’s large availability of water resources in combination with its climatic characteristics allows for the development of many microalgae species. The use of microalgae can potentially contribute to sustainable production in support of the agri-food sector. The nutritional composition (proteins, carbohydrates, fatty acids, vitamins, pigments, and antioxidants) of microalgae along with the ease of producing high biomass yields make them an excellent choice for human and animal nutrition and agriculture. Several species of microalgae have been studied seeking to develop food supplements for pigs, ruminants, poultry, fish, crustaceans, rabbits, and even bees. Important benefits to animal health, production, and improved bromatological and organoleptic characteristics of milk, meat, and eggs have been observed. Based on the functional properties of some microalgae species, foods and supplements have also been developed for human nutrition. Moreover, because microalgae contain essential nutrients, they can be utilized as biofertilizers by replacing chemical fertilizers, which are detrimental to the environment. In view of the above, the study of microalgae is a promising research area for the development of biotechnology and bioeconomy in Colombia.

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Section: Applications In Human Nutritionmentioning

confidence: 75%

Section: Applications In Human Nutritionmentioning

confidence: 99%

Microalgae: Potential for Bioeconomy in Food Systems

et al. 2021

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“…In plants, TAGs are synthesized with structural specificity: SFAs can be found mostly in terminal positions, while UFAs are located at the sn-2 position 64 . There are very few scientific reports on the effect of biotechnological processes on the distribution of FAs in TAGs in food, and these reports are related to the use of lipids in human milk fat substitutes 65 .…”

Section: Resultsmentioning

confidence: 99%

Use of natural biotechnological processes to modify the nutritional properties of bean-based and lentil-based beverages

Cichońska,

Bryś,

Ziarno

2023

Sci Rep

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The market for plant-based beverages (PBBs) is relatively new; hence, to enable its further development, it is important to use new raw materials and improve production technology. The use of natural biotechnological processes can diversify the segment of PBBs, which may offer products with better functionality than those available in the market. Therefore, the present study aimed to determine the effects of fermentation and germination on the nutritional properties of bean-based beverages (BBs) and lentil-based beverages (LBs). The applied processes significantly (p ≤ 0.05) influenced the characteristics of PBBs. Fermentation improved the antioxidant properties (e.g., by increasing the level of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity by 2–6% and 3–7% for BBs and LBs, respectively) and modified the fatty acid (FA) profile of PBBs. This process increased the share of polyunsaturated FAs in the sn2 position in triacylglycerols, which may promote its absorption in the intestine. The simultaneous use of germination and fermentation was most effective in decreasing oligosaccharide content (< 1.55 mg/kg), which may reduce digestive discomfort after consuming PBBs. We recommend that the designing of innovative legume-based beverages should include the application of fermentation and germination to obtain products with probiotic bacteria and improved nutritional properties.

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“…( Zou et al 2016 ). Many efforts have been made to produce the TAG molecules with the C16:0 at the sn -2 position by using engineered microorganisms such as Rhodococcus opacus ( Zhang et al 2020 ) or rewiring the metabolic pathways of oil crops to produce OPO ( van Erp et al 2019 , 2021 ). However, none of these technologies have been commercialized and more economic-viable and environment-friendly technologies are desirable.…”

Section: Discussionmentioning

confidence: 99%

Microalgal glycerol-3-phosphate acyltransferase role in galactolipids and high-value storage lipid biosynthesis

Zou

et al. 2023

Plant Physiology

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Glycerolipids are the most abundant lipids in microalgae, and glycerol-3-phosphate:acyl-CoA acyltransferase (GPAT) plays an important role in their biosynthesis. However, the biochemical and biological functions of algal GPAT remain poorly characterized. Here, we characterized the endoplasmic reticulum (ER)-associated GPAT of the model unicellular green alga Chlamydomonas reinhardtii (CrGPATer). Enzymatic assays indicated CrGPATer is a sn-1 acyltransferase using a variety of acyl-CoAs as the acyl donor. Subcellular localization revealed CrGPATer was associated with ER membranes and lipid droplets. We constructed overexpression and knockdown transgenic C. reinhardtii lines to investigate the in vivo function of CrGPATer. Lipidomic analysis indicated CrGPATer overexpression enhanced the cellular content of galactolipids, especially monogalactosyldiacylglycerol, under nitrogen deficiency stress. Correspondingly, CrGPATer knockdown lines contained lower contents of galactolipids than the control. Feeding experiments with labeled phosphatidic acid revealed that the intermediate of the eukaryotic Kennedy pathway could be used for galactolipid biosynthesis in the chloroplasts. These results provided multiple lines of evidence that the eukaryotic Kennedy pathway mediated by CrGPATer may be involved in galactolipid biosynthesis in C. reinhardtii. Overexpression of CrGPATer significantly increased the content of triacylglycerol and the yield of biomass. Moreover, the content and yield of 1, 3-olein-2-palmitin, a high-value lipid that can be used as an alternative for human milk fat in infant formula, were significantly enhanced in the overexpression transgenic lines. Taken together, this study provided insights into the biochemical and biological functions of CrGPATer and its potential as a genetic engineering target in functional lipid manufacturing.

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Facile and Green Production of Human Milk Fat Substitute through Rhodococcus opacus Fermentation

Cited by 8 publications

References 46 publications

Microalgae: Potential for Bioeconomy in Food Systems

Microalgae: Potential for Bioeconomy in Food Systems

Use of natural biotechnological processes to modify the nutritional properties of bean-based and lentil-based beverages

Microalgal glycerol-3-phosphate acyltransferase role in galactolipids and high-value storage lipid biosynthesis

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