Facile aqueous-phase synthesis of multi-responsive nanogels based on polyetheramines and bisepoxide

Tang, Shuangcheng; Shi, Zengqian; Cao, Yu; He, Wei

doi:10.1039/c3tb00492a

Cited by 26 publications

(41 citation statements)

References 28 publications

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“…10 The successful synthesis consists of two simple steps. Simply by mixing two commercially available water soluble monomers, JT403 and BDE (Scheme 1), we were able to directly generate hydrogel particles in water without the use of any surfactants.…”

Section: Resultsmentioning

confidence: 99%

“…10 Briey, the monomers JT403 and BDE were dissolved in deionized (DI) water to a predetermined total monomer concentration, ranging from 5 wt% to 15 wt%. 10 Briey, the monomers JT403 and BDE were dissolved in deionized (DI) water to a predetermined total monomer concentration, ranging from 5 wt% to 15 wt%.…”

Section: Hydrogel Particle Synthesismentioning

confidence: 99%

“…[1][2][3][4] While macroscale hydrogels continue to be an attractive subject in both applied and fundamental research, there has been a growing interest in developing micro/nanoscale hydrogels in order to harness unique properties that emerge as the length scale of hydrogel material decreases drastically. 10 Particle formation was made possible simply by integrating a step-growth polymerization mechanism with thermally induced phase separation of the in situ generated prepolymers in water. [5][6][7][8][9] Recently, we reported a facile and environmentally friendly strategy to prepare size-tunable hydrogel particles.…”

Section: Introductionmentioning

confidence: 99%

“…Various methods have been utilized to produce micro/nanoscale hydrogel particles, such as photolithographic and micromolding techniques, microuidic preparation, emulsion polymerization, self-assembly followed by crosslinking, etc. 10 We prepared these hydrogel particles in an additive-free and catalyst-free aqueous environment from commercially available monomers, bisepoxide and aliphatic polyetheramine. 10 Particle formation was made possible simply by integrating a step-growth polymerization mechanism with thermally induced phase separation of the in situ generated prepolymers in water.…”

Section: Introductionmentioning

confidence: 99%

“…[5][6][7][8][9] Recently, we reported a facile and environmentally friendly strategy to prepare size-tunable hydrogel particles. 10 Besides responsiveness, another important feature exhibited by these particles is their positive charge, which is particularly appealing for biomedical applications. 10 We prepared these hydrogel particles in an additive-free and catalyst-free aqueous environment from commercially available monomers, bisepoxide and aliphatic polyetheramine.…”

Section: Introductionmentioning

confidence: 99%

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Water-based synthesis of cationic hydrogel particles: effect of the reaction parameters and in vitro cytotoxicity study

et al. 2015

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Micro/nanoscale hydrogel particles are of great interest for biomedical applications, such as carriers for therapeutic delivery. Compared to conventional hydrogel particles that are mainly composed of vinylic monomers, we have introduced a simple methodology to prepare multi-functional cationic hydrogel particles by adopting the epoxy-amine chemistry in water exemplifying "click" characteristics. Herein, we investigate the effects of key reaction parameters, including time, temperature, reactant concentration and amine-epoxy stoichiometric ratio, on the preparation and properties of such hydrogel particles. Our results indicated that the aforementioned parameters could greatly impact the particle formation. The hydrodynamic diameter, surface charge, and morphology of the resultant particles were characterized by dynamic light scattering and scanning electron microscopy. Particle size was inversely correlated with the following reaction parameters: reaction time, temperature, and reactant concentration. This is likely due to the influence of the parameters on the formation of the intermediate thermosensitive prepolymers. Different reaction conditions yielded a wide range of particle surface charges, varying from +47 mV to +71 mV. Morphological analysis also revealed significant effects induced by the variation of reaction time and temperature. All particles exhibited a temperature-dependent swelling property.However, the extent of swelling and sensitivity varied depending on the reaction conditions. Finally, in vitro cytocompatibility studies based on murine RAW264.7 macrophages showed the particle acute cytotoxicity being dose and surface charge dependent. Cytocompatibility of the cationic hydrogel particles was improved by reducing the surface charges with variation of the synthesis conditions. † Electronic supplementary information (ESI) available: SEM images of products collected from reactions based on amino hydrogen to epoxy stoichiometric ratios of 0.5 and 1; thermal responsiveness of the monomer JT403; intensity correlation curves of various hGPs; transmission electron microscopy (TEM) images of hGPs prepared using different reaction times; hGP stability in serum-containing culture medium; and phase contrast image of the non-treated macrophage control culture. See

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Section: Resultsmentioning

confidence: 99%

Section: Hydrogel Particle Synthesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Water-based synthesis of cationic hydrogel particles: effect of the reaction parameters and in vitro cytotoxicity study

et al. 2015

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Comparative study of cytotoxicity and genotoxicity of commercial Jeffamines® and polyethylenimine in CHO‐K1 cells

et al. 2017

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Jeffamines are a family of polymers containing primary amine groups attached to the extremities of polyether backbone which can be used as biomaterials. They have been used in combination with polyethylenimine (PEI) to improve biocompatibility in drug and gene delivery systems. Despite these facts, very few studies have been done on cytotoxicity and genotoxicity of pure Jeffamines or compared with PEI. The present study aimed to evaluate and compare the cytotoxic and genotoxic effects of Jeffamines and PEI in CHO-K1 cells. Specifically, polypropylene oxide 2000 (PPO 2000, Jeffamine D series), polyethylene oxide 1900 (PEO 1900, Jeffamine ED series), branched 25 kDa PEI, and linear 20 kDa PEI were evaluated at different concentrations. Cell viability and proliferation were assessed by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and 5-bromo-2'-deoxyuridine (BrdU) assays, respectively. Genotoxicity was evaluated using single cell gel electrophoresis assay and the cytokinesis-blocked micronucleus assay. PPO 2000 was the most cytotoxic Jeffamine , whereas PEO 1900 did not caused significant cell death at any tested concentration. Branched PEI was more cytotoxic than linear PEI (LPEI) and both were more cytotoxic than Jeffamines . Only PPO 2000 induced DNA damage when evaluated in comet assay probably due to its cytotoxicity. PPO 2000, PEO 1900, and PEI did not increase the frequency of micronuclei when tested at sub-cytotoxic concentrations. This work provides new insights about biocompatibility of Jeffamines and PEI and suggests the genotoxicological safety for further investigations of PEO 1900 in drug and gene delivery systems. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 742-750, 2018.

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Multi-Stimuli-Responsive Polymer Materials: Particles, Films, and Bulk Gels

Cao

Wang

2016

The Chemical Record

176

109

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Stimuli-responsive polymers have received tremendous attention from scientists and engineers for several decades due to the wide applications of these smart materials in biotechnology and nanotechnology. Driven by the complex functions of living systems, multi-stimuli-responsive polymer materials have been designed and developed in recent years. Compared with conventional single- or dual-stimuli-based polymer materials, multi-stimuli-responsive polymer materials would be more intriguing since more functions and finer modulations can be achieved through more parameters. This critical review highlights the recent advances in this area and focuses on three types of multi-stimuli-responsive polymer materials, namely, multi-stimuli-responsive particles (micelles, micro/nanogels, vesicles, and hybrid particles), multi-stimuli-responsive films (polymer brushes, layer-by-layer polymer films, and porous membranes), and multi-stimuli-responsive bulk gels (hydrogels, organogels, and metallogels) from recent publications. Various stimuli, such as light, temperature, pH, reduction/oxidation, enzymes, ions, glucose, ultrasound, magnetic fields, mechanical stress, solvent, voltage, and electrochemistry, have been combined to switch the functions of polymers. The polymer design, preparation, and function of multi-stimuli-responsive particles, films, and bulk gels are comprehensively discussed here.

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Facile aqueous-phase synthesis of multi-responsive nanogels based on polyetheramines and bisepoxide

Cited by 26 publications

References 28 publications

Water-based synthesis of cationic hydrogel particles: effect of the reaction parameters and in vitro cytotoxicity study

Water-based synthesis of cationic hydrogel particles: effect of the reaction parameters and in vitro cytotoxicity study

Comparative study of cytotoxicity and genotoxicity of commercial Jeffamines® and polyethylenimine in CHO‐K1 cells

Multi-Stimuli-Responsive Polymer Materials: Particles, Films, and Bulk Gels

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