Facile biogenic synthesis of silver nanoparticles (AgNPs) by Citrus grandis (L.) Osbeck fruit extract with excellent antimicrobial potential against plant pathogens

Abstract

Biogenic silver nanoparticles (AgNPs) were synthesized by using Citrus grandis (L.) Osbeck fruit extract through a simple one step method by reducing silver nitrate (AgNO 3) at room temperature. Citrus grandis (L.) Osbeck is an indigenously available edible citrus fruit tree in Northeast India and sub-Himalayan foot hills. Formation of AgNPs were confirmed primarily by UV-Vis spectrophotometer and extensively characterized by TEM and XRD. The plasmon resonance at 435 nm confirmed the formation of AgNPs and TEM analysis showed AgNPs with average size of 11 ± 3.57 nm. XRD analysis showed crystalline nature of the Ag NPs with fcc lattice points attributed to (111), (200), (222) and (311) planes. The AgNPs showed excellent antimicrobial activities against both Gram positive (Bacillus cereus) and negative (Pseudomonas syringae pv. syringae) plant pathogens. It exerted MIC value of 20 and 30 µg/mL, respectively against B. cereus and P. syringae pv. Syringae, whereas MBC value of 70 and 80 µg/mL were observed against B. cereus and P syringae pv. Syringae, respectively. The Ag NPs showed no considerable cytotoxicity against murine macrophage RAW264.7 cell line which indicates its further possible application for animal model trial.

Synthesis and Antibacterial Activity of Manganese-Ferrite/Silver Nanocomposite Combined with Two Essential Oils

“…Although this effect was not synergistic, it should be noted that the GO MIC was reduced from 0.14 to 0.04 mg mL −1 in the presence of MnFe 2 O 4 /Ag-NC, and this amount is lower than that obtained by Zabihi et al, who reported an MIC of 0.3 mg mL −1 of GO against E. coli [34]. In terms of the silver concentration, studies have reported that inhibitory doses higher than 10 µg mL −1 of different biogenic AgNPs inhibit P. syringae, either alone or combined with other antibiotics [35][36][37][38]. Thus, by using MnFe 2 O 4 /Ag-NC, we reported a further reduced concentration of silver acting efficiently against P. syringae when combined with essential oils.…”

Fabrication of Silver Nanoparticles in Aqueous Solution by Laser Technique and Study of Their Hemocompatibility and Antibacterial Effects Against Dental Decay Bacteria

“…Using the Scherrer formula, the crystalline size of NPs was calculated (C.s). Table 1 summarises the XRD data for experimental Ag NPs, including peak position, crystalline size variatio n, FWHM, interplanar spacing (dhkl), and miller index (hkl) [33,34].…”

Green synthesis of silver nanoparticle prepared with Ocimum species and assessment of anticancer potential

“…The present cancer therapy challenges can be addressed through nanotechnology and nanoformulations 6 , 7 . Nanomaterials have shown peculiar natures and characteristics such as optical, magnetic, chemical, and mechanical properties, that allow multifaceted applications in food, healthcare, and agriculture 8 , 9 . Metal-based particles like gold (AuNPs) and silver (AgNPs) have drawn considerable interest in infectious diseases and post-surgical infections due to inherent bioactivities and lower possibility of toxicity to normal cells 10 , 11 .…”