Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication

Savelyev, Alexander G.; Sochilina, Anastasia V.; Akasov, Roman; Миронов, А. Н.; Kapitannikova, Alina Y.; Bоrodinа, Tatyana N.; Шолина, Н. В.; Khaydukov, Kirill V.; Zvyagin, Andrei V.; Generalova, Alla N.; Khaydukov, E. V.

doi:10.3389/fbioe.2021.783834

Cited by 7 publications

(8 citation statements)

References 44 publications

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“…In the evaluation of biocompatibility, we observed the influence of mFAECM composite bioinks on HaCaT viability because HaCaT in the surface layer will inevitably contact the mFAECM composite bioinks in the lower layer. The biocompatibility of GelMA and HAMA hydrogels on HaCaT keratinocytes has been confirmed elsewhere, so no further observations were made. − …”

Section: Resultsmentioning

confidence: 95%

3D-Bioprinted Biomimetic Multilayer Implants Comprising Microfragmented Adipose Extracellular Matrix and Cells Improve Wound Healing in a Murine Model of Full-Thickness Skin Defects

Zhang

Lai

et al. 2023

ACS Appl. Mater. Interfaces

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Repairing full-thickness skin defects is a major challenge in clinical practice. Three-dimensional (3D) bioprinting of living cells and biomaterials is a promising technique to resolve this challenge. However, the time-consuming preparation and limited sources of biomaterials are bottlenecks that must be addressed. Therefore, we developed a simple and fast method to directly process adipose tissue into a microfragmented adipose extracellular matrix (mFAECM) as the main component of bioink to fabricate 3D-bioprinted, biomimetic, multilayer implants. The mFAECM retained most of the collagen and sulfated glycosaminoglycans in the native tissue. In vitro, the mFAECM composite demonstrated biocompatibility, printability, and fidelity and could support cell adhesion. In a full-thickness skin defect model in nude mice, cells encapsulated in the implant survived and participated in wound repair after implantation. The basic structures of the implant were maintained throughout wound healing and gradually metabolized. The biomimetic multilayer implants fabricated via mFAECM composite bioinks and cells could accelerate wound healing by promoting the contraction of new tissue inside the wound, collagen secretion and remodeling, and neovascularization. This study provides an approach for improving the timeliness of fabricating 3D-bioprinted skin substitutes and may offer a useful tool for treating full-thickness skin defects.

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Section: Resultsmentioning

confidence: 95%

3D-Bioprinted Biomimetic Multilayer Implants Comprising Microfragmented Adipose Extracellular Matrix and Cells Improve Wound Healing in a Murine Model of Full-Thickness Skin Defects

Zhang

Lai

et al. 2023

ACS Appl. Mater. Interfaces

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“…In spite of the favorable regenerative potential of scaffolds loaded with neurotrophic factors, they still reveal limitations in terms of mimicking the cellular matrix of brain tissue. In particular, to provide satisfied cell distribution within the volume of the scaffold, the cells should be already introduced in hydrogel during the fabrication of the matrix ( Chen et al, 2020 ; Savelyev et al, 2021 ). Furthermore, it is challenging to implant the scaffold in the place of the defect in a minimally invasive way.…”

Section: Discussionmentioning

confidence: 99%

3D-printed hyaluronic acid hydrogel scaffolds impregnated with neurotrophic factors (BDNF, GDNF) for post-traumatic brain tissue reconstruction

Mishchenko

Klimenko

Kuznetsova

et al. 2022

Front. Bioeng. Biotechnol.

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Brain tissue reconstruction posttraumatic injury remains a long-standing challenge in neurotransplantology, where a tissue-engineering construct (scaffold, SC) with specific biochemical properties is deemed the most essential building block. Such three-dimensional (3D) hydrogel scaffolds can be formed using brain-abundant endogenous hyaluronic acid modified with glycidyl methacrylate by employing our proprietary photopolymerisation technique. Herein, we produced 3D hyaluronic scaffolds impregnated with neurotrophic factors (BDNF, GDNF) possessing 600 kPa Young’s moduli and 336% swelling ratios. Stringent in vitro testing of fabricated scaffolds using primary hippocampal cultures revealed lack of significant cytotoxicity: the number of viable cells in the SC+BDNF (91.67 ± 1.08%) and SC+GDNF (88.69 ± 1.2%) groups was comparable to the sham values (p > 0.05). Interestingly, BDNF-loaded scaffolds promoted the stimulation of neuronal process outgrowth during the first 3 days of cultures development (day 1: 23.34 ± 1.46 µm; day 3: 37.26 ± 1.98 µm, p < 0.05, vs. sham), whereas GDNF-loaded scaffolds increased the functional activity of neuron-glial networks of cultures at later stages of cultivation (day 14) manifested in a 1.3-fold decrease in the duration coupled with a 2.4-fold increase in the frequency of Ca2+ oscillations (p < 0.05, vs. sham). In vivo studies were carried out using C57BL/6 mice with induced traumatic brain injury, followed by surgery augmented with scaffold implantation. We found positive dynamics of the morphological changes in the treated nerve tissue in the post-traumatic period, where the GDNF-loaded scaffolds indicated more favorable regenerative potential. In comparison with controls, the physiological state of the treated mice was improved manifested by the absence of severe neurological deficit, significant changes in motor and orienting-exploratory activity, and preservation of the ability to learn and retain long-term memory. Our results suggest in favor of biocompatibility of GDNF-loaded scaffolds, which provide a platform for personalized brain implants stimulating effective morphological and functional recovery of nerve tissue after traumatic brain injury.

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“…Human keratinocytes loaded in the bioink maintained high viability (>95%), thus demonstrating the cytocompatibility of the ink components and the fabrication process. [ 84 ]…”

Section: Generation Of Self‐supporting Perfusable Structuresmentioning

confidence: 99%

“…Human keratinocytes loaded in the bioink maintained high viability (>95%), thus demonstrating the cytocompatibility of the ink components and the fabrication process. [84] Diffusion-induced gelation can also be employed with selfassembly processes to generate high-resolution, perfusable channels. In one example, capillary-like structures were produced based on the diffusion of graphene oxide (GO), which selfassembles with an elastin-like recombinamer, ELK1.…”

Section: Diffusion-induced Interfacial Gelationmentioning

confidence: 99%

“…Free radicals produced by a laser beam diffused into the printed filament, crosslinking an outer shell of the filament. Reproduced under terms of the CC-BY license [84]. Copyright 2021, The Authors, published by Frontiers Media S.A. B) A branched, perfusable network was fabricated by extruding a polylysine (PLL) ink into an oxidized bacterial cellulose (oxBC) solution.…”

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confidence: 99%

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Diffusion‐Based 3D Bioprinting Strategies

Cai,

Kilian,

Ramos Mejia

et al. 2023

Advanced Science

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Abstract3D bioprinting has enabled the fabrication of tissue‐mimetic constructs with freeform designs that include living cells. In the development of new bioprinting techniques, the controlled use of diffusion has become an emerging strategy to tailor the properties and geometry of printed constructs. Specifically, the diffusion of molecules with specialized functions, including crosslinkers, catalysts, growth factors, or viscosity‐modulating agents, across the interface of printed constructs will directly affect material properties such as microstructure, stiffness, and biochemistry, all of which can impact cell phenotype. For example, diffusion‐induced gelation is employed to generate constructs with multiple materials, dynamic mechanical properties, and perfusable geometries. In general, these diffusion‐based bioprinting strategies can be categorized into those based on inward diffusion (i.e., into the printed ink from the surrounding air, solution, or support bath), outward diffusion (i.e., from the printed ink into the surroundings), or diffusion within the printed construct (i.e., from one zone to another). This review provides an overview of recent advances in diffusion‐based bioprinting strategies, discusses emerging methods to characterize and predict diffusion in bioprinting, and highlights promising next steps in applying diffusion‐based strategies to overcome current limitations in biofabrication.

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Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication

Cited by 7 publications

References 44 publications

3D-Bioprinted Biomimetic Multilayer Implants Comprising Microfragmented Adipose Extracellular Matrix and Cells Improve Wound Healing in a Murine Model of Full-Thickness Skin Defects

3D-Bioprinted Biomimetic Multilayer Implants Comprising Microfragmented Adipose Extracellular Matrix and Cells Improve Wound Healing in a Murine Model of Full-Thickness Skin Defects

3D-printed hyaluronic acid hydrogel scaffolds impregnated with neurotrophic factors (BDNF, GDNF) for post-traumatic brain tissue reconstruction

Diffusion‐Based 3D Bioprinting Strategies

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