Facile design and development of nano-clustery graphene-based macromolecular protein hydrogel loaded with ciprofloxacin to antibacterial improvement for the treatment of burn wound injury

Abstract

Graphic abstract Nowadays, awareness about the burn wound is often considered difficult due to bacterial and other organism infections. The facile and eco-friendly preparations of antibiotic-loaded hydrogel-based bio-composites have great attention in the field of wound dressing for burn wound therapy and nursing care. In the present investigation, we have developed ciprofloxacin (CF)-encapsulated graphene-silk fibroin macromolecular hydrogel dressings material with unique chemical and physical properties to achieve the desirable antibacterial efficacy and healing activity. The antibacterial activity of prepared hydrogel was evaluated against bacterial pathogens treated with different concentrations of CF, which have been provided improved antibacterial activity on burn wound infection. In vitro , cytocompatibility evaluations were performed to imply the suitability of hydrogel on fibroblast cells, which has been dramatically related to in vivo wound healing. Furthermore, an in vivo wound healing analysis was carried out using a rat to observe the capability of the CF-incorporated GH/SF hydrogel matrix. Thus, this investigation widely demonstrates the healing ability of prepared hydrogel matrix and could be a significant landmark in the research on burn wound healing applications.

Advancing burn wound treatment: exploring hydrogel as a transdermal drug delivery system

“…The most common approach for combating wound infection is to include antibiotics in hydrogels, which can either kill or impede the growth of pathogens (Gupta et al., 2020 ). Various antibiotics, including tobramycin (Huang et al., 2022 ), ciprofloxacin (Dong et al., 2022 ; Zhu & Chen, 2022 ), gentamycin (Nuutila et al., 2020 ), mupirocin (Chen et al., 2022 ; Han et al., 2022 ), and vancomycin (Nuutila et al., 2020 ), have been incorporated into hydrogels to combat microbial infection in burn wounds. For instance, a tobramycin-loaded self-healing hydrogel was developed for the treatment of P. aeruginosa -infected burn wound healing.…”

Advances in preparation and application of antibacterial hydrogels

“…Zhu et al prepared graphene/silk fibroin composite hydrogels and loaded CIP (Fig. 3 a) [ 66 ]. The antibacterial activities of CIP-loaded graphene/SF hydrogel against Pseudomonas aeruginosa ( P. aeruginosa ) and S. aureus were detected by the zone of inhibition test.…”

“…3 a Schematic synthesis of graphene/silk cellulose composite hydrogel loaded with Ciprofloxacin (CIP) and its application in wound healing. Reproduced with permission [ 66 ]. Copyright 2021, Springer Nature.…”

Unraveling the New Perspectives on Antimicrobial Hydrogels: State-of-the-Art and Translational Applications

“…In addition, antimicrobial agents are released directly at the infected site, avoiding systemic toxicity. There exist five main groups of antimicrobial agents incorporated into hydrogels: antibiotics, biological extracts, metal nanoparticles, and AMPs [ 82 , 83 , 84 , 85 ].…”

“…The result was an effective, injectable, and self-healing AMH with sustained drug release and potential proteins/growth factors that accelerate the healing process. Ciprofloxacin-encapsulated graphene-silk fibroin macromolecular hydrogels have been designed for burn wound injury and ciprofloxacin and ginsenoside Rh2-loaded poly (lactic-co-glycolic acid)-microsphere thermo-sensitive hydrogel to treat S. aureus skin infections [ 82 , 93 ]. Lastly, Giglio et al designed two electrosynthesized hydrogel coatings loaded with ciprofloxacin that are effective for preventing titanium implant-associated infections frequently related to orthopedic surgery [ 94 ].…”