Facile detection of botulinum neurotoxin using LSPR nanosensor based on Langmuir–Blodgett films of gold nanoparticles

Huong, Nguyen Thanh; Nhiem, Ly Tan

doi:10.1039/d3ra05386e

RSC Adv.

2023

DOI: 10.1039/d3ra05386e

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Facile detection of botulinum neurotoxin using LSPR nanosensor based on Langmuir–Blodgett films of gold nanoparticles

Nguyen Thanh Huong,

Ly Tan Nhiem

Abstract: In this exploratory study, Langmuir–Blodgett (LB) films of gold nanoparticles (Au NPs) were utilized for the first time to detect botulinum neurotoxin (BoNT) based on localized surface plasmon resonance (LSPR), acting as biosensors.

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2024

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Cited by 4 publications

References 38 publications

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Wearable Temperature Sensor Enhanced Volatilomics Technique for Swift and Convenient Detection of Latrogenic Botulism

Li,

Yan,

et al. 2024

Advanced Science

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Accurately assessing potential side effects following botulinum neurotoxin (BoNT) injection remains a formidable challenge. To address this issue, an innovative approach is developed that combines a wearable temperature sensor with a sophisticated volatilomics technique, aimed at facilitating the rapid and convenient prediction of potential physical discomfort related to latrogenic botulism. The investigation identifies five volatile organic compounds (VOCs)—acetone, styrene, ethanol, 2‐pentanone, and n‐butano—as promising markers indicative of BoNT poisoning. Specifically, a handheld breath analyzer, featuring a yttrium stabilized zirconia (YSZ)‐based gas sensor array, alongside a wearable temperature sensor integrated with a bio‐compatible methacrylated gelatin (GelMA) sensing film, are developed to simultaneously monitor breath signal variations and body temperature fluctuations. Preliminary animal testing validates the effectiveness of the integrated approach, achieving an accuracy exceeding 91.2% in early detection of physical discomfort associated with BoNT poisoning. These promising findings represent a significant advancement towards the early identification of BoNT‐related issues, enabling timely intervention and improved management strategies.

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Wearable Temperature Sensor Enhanced Volatilomics Technique for Swift and Convenient Detection of Latrogenic Botulism

Li,

Yan,

et al. 2024

Advanced Science

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A versatile immunoassay based on functionalized nanoparticles for botulinum neurotoxin detection and sensor development

Nguyen,

et al. 2024

Discov Appl Sci

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Botulinum neurotoxins (BoNTs) serotypes A and B are the two most common of the four BoNTs that cause the high mortality botulism disease in individuals consuming contaminated foods. The gold standard assay for BoNT detection is the live mouse bioassay, which has several major disadvantages, including tedious procedures and animal sacrifice requirements. In this study, we developed an immuno-based assay using magnetic streptavidin nanoparticles (mSNP) functionalized with specific synthetic biotinylated, 6xHis-tagged peptide substrates (Peptides A, PA and B, PB) designed for BoNT/A and BoNT/B proteolytic reactions, respectively. In the presence of active toxins that possess endopeptidase activity, upon cleavage, the released fragments with His-tag were dotted on a blotting membrane, ultimately producing color signals after incubation with anti-His antibody, alkaline phosphatase (AP)-conjugated antibody, and then AP substrates. The results showed that the efficiency of peptide-mSNP complex formation reached up to 81%, and the dot blot immunoassay allowed peptide detection from 10 ng of His-tagged peptides. Preliminary testing with the extracellular extracts from the isolated Clostridium botulinum strains indicated that the botulinum toxin in the 2020 botulism outbreak in Vietnam belonged to serotype A, the most potent BoNT. The established assay could be applied to construct a portable biosensor for BoNT detection and a high throughput device to screen potential BoNT inhibitors for drug development.

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Preparation of Langmuir-Blodgett composite films based on sericin protein and silk fibroin with good surface-enhanced Raman scattering and photovoltaic properties

Zhao,

Wang,

Wei

et al. 2024

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Facile detection of botulinum neurotoxin using LSPR nanosensor based on Langmuir–Blodgett films of gold nanoparticles

Abstract: In this exploratory study, Langmuir–Blodgett (LB) films of gold nanoparticles (Au NPs) were utilized for the first time to detect botulinum neurotoxin (BoNT) based on localized surface plasmon resonance (LSPR), acting as biosensors.

Cited by 4 publications

References 38 publications

Wearable Temperature Sensor Enhanced Volatilomics Technique for Swift and Convenient Detection of Latrogenic Botulism

Wearable Temperature Sensor Enhanced Volatilomics Technique for Swift and Convenient Detection of Latrogenic Botulism

A versatile immunoassay based on functionalized nanoparticles for botulinum neurotoxin detection and sensor development

Preparation of Langmuir-Blodgett composite films based on sericin protein and silk fibroin with good surface-enhanced Raman scattering and photovoltaic properties

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