Facile Differential UV Determination of Steroids with Conjugated Ketone Chromophoresvia Lithium Borohydride Reduction

Differential Borohydride Assay for Conjugated Ketosteroids: Improvement with Propylene Glycol

“…3-0ne-1,4-diene steroids are less than 10% reduced, limiting this procedure. The procedure of Chafetz et al (2), using lithium borohydride, also reduces 1,4-diene steroids. Two samples of 3-5-year-old lithium borohydride gave incomplete reductions of halcinonide and triamcinolone after the recommended procedure was modified for equimolar lithium borohydride.…”

“…Keyphrases Steroids, 3-one-4-ene-effect of added propylene glycol on differential UV analysis UV spectrophotometry, differentialanalysis of various 3-one-4-ene steroids, effect of added propylene glycol 0 Propylene glycol-effect of addition on differential UV analysis of various 3-one-4-ene steroids While developing a differential UV assay for the 3-one-4-ene steroid halcinonide' (I), a topical anti-inflammatory agent, sodium borohydride reagent frequently gave incomplete reduction of the steroid. The method was adapted from the procedure of Gorog (l), as modified by Chafetz et al (2), for quantitating conjugated 3-ketosteroids. This differential assay involves measuring the absorbance of an aliquot of methanolic steroid solution containing sodium borohydride, decomposed prior to the addition of steroid.…”

“…The method was adapted from the procedure of Gorog (l), as modified by Chafetz et al (2), for quantitating conjugated 3-ketosteroids. This differential assay involves measuring the absorbance of an aliquot of methanolic steroid solution containing sodium borohydride, decomposed prior to the addition of steroid.…”