Facile Fabrication of a Novel and Reusable 3D Laccase Reactor for Efficient Removal of Pollutants from Wastewater

Zou, Yulin; Ran, Fanpeng; Huang, Qian; Liu, Xiaoyan; Zhang, Haixia

doi:10.1007/s10562-019-02732-8

Cited by 8 publications

(5 citation statements)

References 53 publications

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“…and found enhanced decolorization in the presence of a mediator. Zuo et al [13] reported 84.9% decolorization of bromocresol by Pleurotusostreatus HAUCC 162 and noted the effect of mediator HBT in increasing the decolorization. Enzymes immobilization has been observed to make the enzymes reusable, provide more stability and resistance under diverse conditions, and improve the catalytic activity of laccases [24].…”

Section: Discussionmentioning

confidence: 99%

“…High catalytic efficiency is another key feature of the enzyme that has been utilized in the bioremediation of dye effluent, sulfonamide, and other pollutants. This bioremediation ismediated by thelaccase mediator system (LMS) [13]. Laccase has emerged as a significant enzyme in the mycoremediation of grey-water treatment as it substantially reduces the chemical oxygen demand (COD) and biological oxygen demand (BOD), and solids present in grey-water [14].…”

Section: Introductionmentioning

confidence: 99%

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Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Sayyed

Bhamare²,

Sapna

et al. 2020

PLoS ONE

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Although laccase has been recognized as a wonder molecule and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its large-scale application. Thus,this study aims to select high yielding fungal strains and optimize the production, purification, and kinetics of laccase of Aspergillus sp. HB_RZ4. The results obtained indicated that Aspergillus sp. HB_RZ4 produced a significantly large amount of laccase under meso-acidophilic shaking conditions in a medium containing glucose and yeast extract. A 25 μM CuSO 4 was observed to enhance the enzyme yield. The enzyme was best purified on a Sephadex G-100 column. The purified enzyme resembled laccase of A. flavus. The kinetics of the purified enzyme revealed high substrate specificity and good velocity of reaction,using ABTS as a substrate. The enzyme was observed to be stable over various pH values and temperatures. The peptide structure of the purified enzyme was found to resemble laccase of A. kawachii IFO 4308. The fungus was observed to decolorize various dyes independent of the requirement of a laccase mediator system. Aspergillus sp. HB_RZ4 was observed to be a potent natural producer of laccase, and it decolorized the dyes even in the absence of a laccase mediator system. Thus, it can be used for bioremediation of effluent that contains non-textile dyes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Sayyed

Bhamare²,

Sapna

et al. 2020

PLoS ONE

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show abstract

“…20,[34][35][36] Previous studies have indicated that physical adsorption led to laccase easily leaking out, causing enzyme loss and poor stability. 37,38 Covalent immobilization can bind laccase to the support tightly, hence avoiding the leakage of enzyme molecules, but attachment may influence the conformation of laccase and decrease its catalytic activity. [39][40][41][42] Therefore, the development of immobilized laccase with high activity and stability for the biodegradation of dyestuffs is seriously considered.…”

Section: Introductionmentioning

confidence: 99%

Improved performance of immobilized laccase for catalytic degradation of synthetic dyes using redox mediators

Zhu

Liu

et al. 2022

New J. Chem.

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“…High catalytic efficiency is another key feature of the enzyme in the bioremediation of dye effluent, sulphonamide, and other pollutants. This bioremediation is mediated by a laccase mediator system (LMS) [13]. Laccase has emerged as significant enzyme in mycoremediation of grey-water treatment as it substantially reduces the COD, BOD, and solids present in grey-water [14].…”

Section: Introductionmentioning

confidence: 99%

“…and have found enhanced decolorization in the presence of a mediator. Zuo et al[13] have reported 84.9% decolorization of bromocresol by Pleurotus ostreatus HAUCC 162 and have claimed the involvement of the effect of mediator HBT in increasing decolorization. Enzymes immobilization offers the reuse of enzyme, more stability, and resistance under diverse conditions and improves the catalytic activity of laccases…”

mentioning

confidence: 99%

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Bhamare¹,

Sayyed

Marraiki

et al. 2020

Preprint

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Although laccase has been recognized as a wonder molecule, and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its larger-scale applications. Hence the present research was aimed to select high yielding fungal strains and to optimize the production, purification, and kinetics of laccase of Aspergillus sp. HB_RZ4. Aspergillus sp. HB_RZ4 produced a copious amount of laccase on under meso-acidophillic shaking conditions in a medium containing glucose and yeast extract. A 25 µM of CuSO 4 enhanced the enzyme yield. The enzyme was best purified on Sephadex G-100 column. Purified enzyme resembled with the laccase of A. flavus. Kinetics of purified enzyme revealed the high substrate specificity and good velocity of reaction with ABTS as substrate. The enzyme was stable over a wide range of pH and temperature. The peptide structure of the purified enzyme resembled with the laccase of A. kawachii IFO 4308. The fungus decolorized various dyes independent of the requirement of a laccase mediator system (LMS). Aspergillus sp. HB_RZ4 came out as a potent natural producer of laccase, it decolorized the dyes even in absence of LMS and thus can be used for bioremediation.

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Facile Fabrication of a Novel and Reusable 3D Laccase Reactor for Efficient Removal of Pollutants from Wastewater

Cited by 8 publications

References 53 publications

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Improved performance of immobilized laccase for catalytic degradation of synthetic dyes using redox mediators

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

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