Facile Fabrication of Bio‐ and Dual‐Functional Poly(2‐oxazoline) Bottle‐Brush Brush Surfaces

Du, Yunhao; Zhang, Tao; Gieseler, Dan; Schneider, Maximilian; Hafner, Daniel; Sheng, Wenbo; Li, Wei; Lange, F. F.; Wegener, Erik; Amin, Ihsan; Jordan, Rainer

doi:10.1002/chem.201905326

Cited by 19 publications

(17 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the following equation where σ (chains/nm 2 ) is the grafting density, h (nm) is the dry layer thickness, ρ (1.0 g/cm 3 ) is the density of the PPOx layers, N A is Avogadro’s constant, and M n (g/mol) is the number average molar mass of the grafted polymers. The grafting densities of PnPrOx and PiPrOx were calculated to be 0.53 and 0.45 chains/nm 2 , relatively higher than the results of other grafting methods. , …”

Section: Resultsmentioning

confidence: 63%

Thermoresponsive Poly(2-propyl-2-oxazoline) Surfaces of Glass for Nonenzymatic Cell Harvesting

Wang

Ren

Bernaerts

et al. 2020

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

As one of the nonenzymatic cell-harvesting technologies, a thermal-responsive surface based on poly(2-oxazoline)s has achieved initial success in supporting the adhesion and thermal-induced detachment of animal cells. However, because of the laborious preparation procedure, this technique was only limited to research purposes. In this work, through using poly(glycidyl methacrylate) (PGMA) as the anchor layer, poly(2-propyl-2-oxazoline)s (PPOx) were grafted onto glass wafers through a facile two-step coating and annealing procedure for nonenzymatic cell harvesting. In the first step, the piranha solution-activated glass wafers were immersed into the chloroform solution of PGMA and then annealed for a given period of time to immobilize PGMA onto the glass wafers through the bonding between epoxy groups and hydroxyl groups. In the second step, the PGMA-coated glass wafers were further immersed into the chloroform solution of carboxyl-functionalized PPOx. After annealing, PPOx were immobilized onto the PGMA layer through the bonding between carboxyl groups and the residual epoxy groups. Atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry were used to characterize the modified glass wafers. The results of cytocompatibility evaluation showed that the PPOx-coated glass wafers were almost nontoxic and were able to support the adhesion and proliferation of L929 cells well. By lowering the temperature to 8 °C, L929 and Vero cells were successfully detached from the PPOx-coated glass wafers without any enzymatic treatment. Further cultivation has demonstrated that the cooling procedure had little effect on cell viability, and the cells still retained good viability after harvesting.

show abstract

Section: Resultsmentioning

confidence: 63%

Thermoresponsive Poly(2-propyl-2-oxazoline) Surfaces of Glass for Nonenzymatic Cell Harvesting

Wang

Ren

Bernaerts

et al. 2020

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…A molecule with similar antifouling properties as PEG but with improved chemical stability is poly(oxazoline) (POx). − The derivatives poly(2-methyl-2-oxazoline) (PMeOx) and poly(2-ethyl-2-oxazoline) (PEtOx) are widely tested alternatives to PEG in order to establish polyhydrophilic surfaces. ,,− Although POx is easier to produce in terms of equipment, chemicals, yields, and coating, , there are mainly in vitro studies at the moment, and specific therapeutic approaches are missing. , Further tests under physiological conditions are needed to establish POx as material for biomedical devices.…”

Section: Recent Efforts To Reduce Platelet-surface Activationmentioning

confidence: 99%

Modulation of Platelet-Surface Activation: Current State and Future Perspectives

Apte

Börke

Rothe

et al. 2020

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Modulation of platelet-surface activation is important for many biomedical applications such as in vivo performance, platelet storage, and acceptance of an implant. Reducing platelet-surface activation is challenging because they become activated immediately after short contact with nonphysiological surfaces. To date, controversies and open questions in the field of platelet-surface activation still remain. Here, we review state-of-the-art approaches in inhibiting platelet-surface activation, mainly focusing on modification, patterning, and methodologies for characterization of the surfaces. As a future perspective, we discuss how the combination of biochemical and physiochemical strategies together with the topographical modulations would assist in the search for an ideal nonthrombogenic surface.

show abstract

“…The group of Jordan exploited surface-initiated Cu 0 -mediated controlled radical polymerization (SI-Cu 0 CRP) [38][39][40] to synthesize poly(2-isopropenyl-2-oxazoline) (PIPOXA) brushes, which bear a polymerizable 2-oxazoline group on each monomer unit, and act as precursor layers for the subsequent grafting from of PMOXA chains by CROP. [41] This two-step fabrication enabled the synthesis of surface-attached PIPOXA-g-PMOXA bottlebrushes of considerably high thickness, while by varying the distance between the Cu 0 -coated plate used as catalyst source and the initiator-bearing substrate [42] morphologically different PAOXA-based brush gradients could be easily and quickly fabricated.…”

Section: Surface Functionalization Strategiesmentioning

confidence: 99%

Fabrication of Biopassive Surfaces Using Poly(2‐alkyl‐2‐oxazoline)s: Recent Progresses and Applications

Trachsel

Romio

Ramakrishna

et al. 2020

Adv Materials Inter

View full text Add to dashboard Cite

monomer composition enables to obtain hydrophilic and bioinert and/or amphiphilic PAOXA species displaying thermoresponsive properties. [6] Simultaneously, careful choice of initiator and/or terminator agents gives access to end-functional or heterobifunctional polymers, which can be directly applied for surface modification, or used as building blocks to yield structurally more complex adsorbates targeting specific substrates or generating well-defined polymer architectures at the interface. [7-9] Among the most recent studies focusing on the fabrication of biopassive PAOXA films, most of them involved grafting of pre-synthesized adsorbates by exploiting functional anchors that are specific for the target substrate to passivate. In other works, the growth of a PAOXAbased film was triggered from the substrate itself, through surface-initiated CROP (SI-CROP), while interest has been rising in applying plasma polymerization of 2-oxazolines to generate robustly attached coatings on an array of different supports. Through these strategies, and with the general objective of suppressing nonspecific interaction with biological entities, a broad range of biomaterials, ranging from inorganic supports, plastics and nanomaterials of diverse composition have been successfully functionalized with PAOXAs. While during the first decade of 2000s efforts were spent in dissecting the fundamental properties of PAOXAs assemblies on surfaces, [3,10,11] during the past five years their application in different coating formulations for biomaterials has been progressively expanding, and at least some of them are probably mature for a direct involvement of industry. In this progress report, we critically review the most recent studies focusing on the fabrication of PAOXA coatings, especially concentrating on the generation of bioinert surfaces, which probably represents their most appealing and technologically relevant application. 2. Composition of Bioinert PAOXA-Based Surfaces Among the different PAOXA species that were applied on surfaces to generate coatings, hydrophilic poly(2-methyl-2-oxazoline) (PMOXA) and poly(2-ethyl-2-oxazoline) (PEOXA) showed the most pronounced inertness towards nonspecific interactions with serum proteins, cells and bacteria. A recent study by Morgese et al. identified the superior biopassivity of linear Poly(2-alkyl-2-oxazoline)s (PAOXAs) are emerging among the most promising nonionic alternatives to poly(ethylene glycol)s (PEGs), specifically in the modification and functionalization of biomaterials. Due to their chemical tailorability and robustness, coupled to their relatively easy synthesis, PAOXAs are increasingly applied as adsorbates to generate bioinert surfaces that prevent nonspecific contamination by proteins, cells and bacteria. Passivation of medical devices, sensors and cell-sensitive platforms with PAOXAs enables a nearly quantitative suppression of nonspecific biological contamination, while biopassivity is maintained over longer incubation times than those recorded for more degradable PEG-b...

show abstract

Facile Fabrication of Bio‐ and Dual‐Functional Poly(2‐oxazoline) Bottle‐Brush Brush Surfaces

Cited by 19 publications

References 62 publications

Thermoresponsive Poly(2-propyl-2-oxazoline) Surfaces of Glass for Nonenzymatic Cell Harvesting

Thermoresponsive Poly(2-propyl-2-oxazoline) Surfaces of Glass for Nonenzymatic Cell Harvesting

Modulation of Platelet-Surface Activation: Current State and Future Perspectives

Fabrication of Biopassive Surfaces Using Poly(2‐alkyl‐2‐oxazoline)s: Recent Progresses and Applications

Contact Info

Product

Resources

About