Facile modification of gelatin-based microcarriers with multiporous surface and proliferative growth factors delivery to enhance cell growth

Huang, Sha; Wang, Yijuan; Deng, Tianzheng; Jin, Fang; Liu, Shouxin; Zhang, Yongjie; Feng, Feng; Jin, Yan

doi:10.1016/j.jallcom.2007.06.045

Cited by 6 publications

(2 citation statements)

References 34 publications

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“…P pro MPs with a flower-like surface texture were observed (Figure 2c). The presence of many lamellate protrusions greatly increases the MP surface roughness, which is ideal for attachment to biological (cellular) structures [24]. Previous studies have demonstrated the role of topography (roughness and chemistry) in cell-material interactions [25], and as ES has been utilized to prepare biomaterial coatings (e.g., using PCL), enhancing the surface-cell interaction will be achievable using P pro MPs.…”

Section: Resultsmentioning

confidence: 99%

Tuning Microparticle Porosity during Single Needle Electrospraying Synthesis via a Non-Solvent-Based Physicochemical Approach

et al. 2015

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Porous materials, especially microparticles (MP), are utilized in almost every field of engineering and science, ranging from healthcare materials (drug delivery to tissue engineering) to environmental engineering (biosensing to catalysis). Here, we utilize the single needle electrospraying technique (as opposed to complex systems currently in development) to prepare a variety of poly(ε-caprolactone) (PCL) MPs with diverse surface morphologies (variation in pore size from 220 nm to 1.35 µm) and architectural features (e.g., ellipsoidal, surface lamellar, Janus lotus seedpods and spherical). This is achieved by using an unconventional approach (exploiting physicochemical properties of a series of non-solvents as the collection media) via a single step. Sub-micron pores presented on MPs were visualized by electron microscopy (demonstrating a mean MP size range of 7-20 µm). The present approach enables modulation in morphology and size requirements for specific applications (e.g., pulmonary delivery, biological scaffolds, multi-stage drug delivery and biomaterial topography enhancement). Differences in static water contact angles were observed between smooth and porous MP-coated surfaces. This reflects the hydrophilic/hydrophobic properties of these materials.

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Section: Resultsmentioning

confidence: 99%

Tuning Microparticle Porosity during Single Needle Electrospraying Synthesis via a Non-Solvent-Based Physicochemical Approach

et al. 2015

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“…The pore size of the composite cryogels was intermediate with an average pore size of 47 μm for 3.5GC and 32 μm for 4.5GC cryogels. Porosity is a necessity for dermal substitutes as the interconnectivity allows cellular migration, spatial distribution of cells, efficient diffusion of gases and nutrients, and vascular ingrowth, resulting in the faster formation of tissue. , The trend in pore size observed in our study is a direct effect of the total polymer concentration. As the polymer concentration increases, the crosslinking density of polymer molecules also increases due to the increased availability of crosslinking junctions.…”

Section: Resultsmentioning

confidence: 51%

Clove Oil-Incorporated Antibacterial Gelatin–Chitosan Cryogels for Tissue Engineering: An In Vitro Study

Saha

Tayalia

2022

ACS Biomater. Sci. Eng.

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Infections are a leading cause of mortality and amputations among patients with burns and chronic wounds, respectively. Moreover, the extensive use of antibiotics has led to the rapid spreading of drug resistance among microorganisms. Alternatively, plant-derived natural products, which have been used as traditional therapies for several centuries, are recently gaining popularity as they are relatively affordable and easily available in many developing countries where modern medications are expensive or unavailable. In this study, clove essential oil is used for its antimicrobial property and is further incorporated into cryogels to increase its bioavailability and prolong its bioactivity. The oilincorporated cryogels are macroporous, biodegradable, possess mechanical properties similar to commercial skin substitutes, are cytocompatible, antibacterial, and allow long-term sustained release of oil for up to at least 14 days. Additionally, clove oil aids the faster closure of in vitro scratch wounds by improving the migration of fibroblasts. This work presents a novel, bioactive scaffold that has the potential to be used as a dermal substitute and serves as an alternative to commercial skin substitutes.

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Fabrication of uniform-sized poly-ɛ-caprolactone microspheres and their applications in human embryonic stem cell culture

Lam

Toh

et al. 2015

Biomed Microdevices

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The generation of liquefied poly-ɛ-caprolactone (PCL) droplets by means of a microfluidic device results in uniform-sized microspheres, which are validated as microcarriers for human embryonic stem cell culture. Formed droplet size and size distribution, as well as the resulting PCL microsphere size, are correlated with the viscosity and flow rate ratio of the dispersed (Q d) and continuous (Q c) phases. PCL in dichloromethane increases its viscosity with concentration and molecular weight. Higher viscosity and Q d/Q c lead to the formation of larger droplets, within two observed formation modes: dripping and jetting. At low viscosity of dispersed phase and Q d/Q c, the microfluidic device is operated in dripping mode, which generates droplets and microspheres with greater size uniformity. Solutions with lower molecular weight PCL have lower viscosity, resulting in a wider concentration range for the dripping mode. When coated with extracellular matrix (ECM) proteins, the fabricated PCL microspheres are demonstrated capable of supporting the expansion of human embryonic stem cells.

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Facile modification of gelatin-based microcarriers with multiporous surface and proliferative growth factors delivery to enhance cell growth

Cited by 6 publications

References 34 publications

Tuning Microparticle Porosity during Single Needle Electrospraying Synthesis via a Non-Solvent-Based Physicochemical Approach

Tuning Microparticle Porosity during Single Needle Electrospraying Synthesis via a Non-Solvent-Based Physicochemical Approach

Clove Oil-Incorporated Antibacterial Gelatin–Chitosan Cryogels for Tissue Engineering: An In Vitro Study

Fabrication of uniform-sized poly-ɛ-caprolactone microspheres and their applications in human embryonic stem cell culture

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