Facile one-pot synthesis of fluorescent hyperbranched polymers for optical detection of glucose

Jiang, Tengteng; Jiang, Guohua; Wang, Xiaohong; Dong, Yue; Wei, Zhen; Li, Xia; Tang, Bolin

doi:10.1080/15685551.2014.907610

Cited by 13 publications

(4 citation statements)

References 36 publications

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“…As shown in Table I , both the molecular weight and the degree of branching are relatively low in comparison with some reports on the one-pot-synthesized hyperbranched polymers ( 37 , 38 ) but are in agreement with a previous report utilizing the same CBA, ABOL and similar reaction procedure ( 29 ). Although this previous report concluded no significant difference in the branched vs linear PAA, we note significant increase in molecular weight of BA-containing PAAs reported in this study in comparison with previously reported linear BA-containing PAA ( 16 ).…”

Section: Resultssupporting

confidence: 91%

Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s

Hujaya

Engbersen

2015

Pharm Res

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PurposeTo investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells.MethodsCopolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films.ResultsPVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10–100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells.ConclusionsResponsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants.Graphical AbstractLayer-by-Layer Assembly

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Section: Resultssupporting

confidence: 91%

Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s

Hujaya

Engbersen

2015

Pharm Res

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“…16 In particular, surface chemistry engineering is deemed to be the most realistic strategy for designing high emission intensity materials since lowing pH or oxidation protocols can be fatal for in vivo systems due to protein denaturation and ROS generation. 16 Plenty of micro molecules such as mannose, 18 3-aminobenzeneboronic acid, 19 and 4carbomethoxy pyrrolidone 20 have been successfully applied to PAMAM to fabricate high inherent blue fluorescent PAMAM dendrimers with excellent biocompatibility and imaging capabilities. However, those chemistry engineered blue fluorescent dendrimers usually require UV excitation, which poses major limitations in its application since UV is harmful to living cells and biological systems.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Fluorescent Poly(amidoamine) Dendrimer for Traceable and Controlled Drug Delivery

Wang

Walker

et al. 2019

Biomacromolecules

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Poly(amidoamine) dendrimer (PAMAM) is well known for its high efficiency as a drug 3 delivery vehicle. However, the intrinsic cytotoxicity and lack of a detectable signal to facilitate 4 tracking have impeded its practical applications. Herein, we have developed a novel label-free 5 fluorescent and biocompatible PAMAM derivative by simple surface modification of PAMAM 6 using acetaldehyde. The modified PAMAM possessed a strong green fluorescence, which was 7 generated by the C=N bonds of the resulting Schiff Bases via n-π* transition, while the intrinsic 8 cytotoxicity of PAMAM was simultaneously ameliorated. Through further PEGylation, the 9 fluorescent PAMAM demonstrated excellent intracellular tracking in human melanoma 10 SKMEL28 cells. In addition, our PEGylated fluorescent PAMAM derivative achieved 11 enhanced loading and delivery efficiency of the anticancer drug doxorubicin (DOX) compared 12 to the original PAMAM. Importantly, the accelerated kinetics of DOX-encapsulated 13 fluorescent PAMAM nanocomposites in an acidic environment facilitated intracellular drug 14 release, demonstrating comparable cytotoxicity to that of the free-form doxorubicin 15 hydrochloride (DOX•HCl) against melanoma cells. Overall, our label free fluorescent 16 PAMAM derivative offers a new opportunity of traceable and controlled delivery for DOX and 17 other drugs of potential clinical importance.

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“…From a clinical perspective, there is a need for high-sensitivity, low-cost methods with good reproducibility for the rapid and accurate determination of glucose. 1,2 Much work has been carried out to develop methods to monitor glucose concentrations in the human body. Of these, enzyme-free electrochemical sensors have become highly desirable as these avoid the use and sophisticated manufacture of glucose oxidase (GODx) and are aimed at the direct oxidation of glucose in blood samples.…”

Section: Introductionmentioning

confidence: 99%