Facile one‐pot synthesis of silica‐based lipase nanocatalysts for improving stability

Abstract

The inactivation of lipases caused by organic solvents, high temperature, extremely acidic and alkaline requires a high stability. In this work, a novel one-pot synthesis for the lipase immobilisation on silica nanoparticles was reported. The optimal amounts of (3-ammonia propyl) triethoxysilane (APTES) and ammonia were studied. The apparent K m value of the immobilised Candida rugosa lipase (CRL) was lower than that of free enzyme, showing affinity of the immobilised CRL to its substrate had increased. The results of stability test showed that the immobilised lipase was more stable than free enzyme at different temperatures and pH values. In particular, the immobilised CRL kept 96% activity at 90°C, while the free enzyme only remained 75% activity. Immobilised lipase had high catalytic efficiency, enhanced stability and recyclable usability compared to free enzymes, because of the cross-linking between the protein and the rigid carrier. Therefore, the immobilised method would be beneficial to improving the activity and stability of enzyme universally.

Carboxylated Cellulose Nanocrystals Developed by Cu-Assisted H2O2 Oxidation as Green Nanocarriers for Efficient Lysozyme Immobilization

“…It is well-known that the catalytic activity of the immobilized enzymes depends greatly upon the supporting material. The extremely small size and the large specific surface area of nanoparticles endow them as nanocarriers with minimized diffusional restrictions and maximized enzyme loading capacities, thereby enhancing enzyme activities. , …”

“…The extremely small size and the large specific surface area of nanoparticles endow them as nanocarriers with minimized diffusional restrictions and maximized enzyme loading capacities, thereby enhancing enzyme activities. 3,4 In most research associated with enzyme immobilization, synthetic colloidal nanoparticles and chemical-based procedures with significant environmental and healthcare side effects are employed to improve the efficacy of nanocarrier systems or develop multifunctional nanoplatforms. Bio-inspired cellulose nanocrystals (CNCs) are expected to offer many interesting solutions to the environmental challenges facing nanocarriers as a result of their unique characteristics of non-toxicity, ease of fabrication and modification, biodegradability, and biocompatibility.…”