Facile preparation of a pH-sensitive nano-magnetic targeted system to deliver doxorubicin to tumor tissues

Abstract

We have developed a drug-loaded, pHsensitive, nano-magnetic targeted system (DPNTS) for delivering doxorubicin (DOX) to tumor tissues through a facile route. Iron oxide (Fe 3 O 4 ) nanoparticles were used as magnetically-responsive carriers, polyethyleneglycol (PEG) as the surface-modifying agent, and polyethyleneimine (PEI) as the drugloading site whose primary amine reacts with the 13-carbonyl of DOX. The prepared DPNTS was within 20 nm and had good stability in dispersion and superparamagnetic properties. DOX was grafted to PEG/PEI@Fe 3 O 4 at up to 85 %. During in vitro release studies, nearly 81 % DOX was released from DPNTS within 72 h at pH 4.5, compared with only 28 % at pH 7.4.

Synthesis and in vitro evaluation of pH-sensitive magnetic nanocomposites as methotrexate delivery system for targeted cancer therapy

“…Many types of novel MTX delivery systems have been reported, such as nanoparticles [7][8][9], microspheres [10], liposomes [11], polymeric micelles [12] and miscellaneous multiparticulate systems [13]. Among these, nanoparticles, especially the magnetic iron oxide (Fe3O4) nanoparticles seem to be a promising tool for site specific delivery [14,15], based on the fact that they will release the anticancer drug on tumor cells owing to the enhanced permeability and retention (EPR) effect [16,17] and passive drug targeting. Furthermore, recently layered double hydroxide (LDH) nanoparticles have been proven effective for site-specific delivery of anticancer drugs to tumor and reduce its side effects, due to their inherent properties such as high biocompatibility [18], low cytotoxicity [19], the ability to accommodate various biologically important molecules including genes and drugs [20][21][22], and the partial dissolution of LDH layers in endosome that not only stops the passive control release of drugs, but also buffers the excess protons, which helps the drugs to escape from endosome, improves the viability of drugs in cytoplasm, and enhances the delivery efficacy [23].…”

In vitro and in vivo chemo-phototherapy of magnetic TiO2 drug delivery system formed by pH-sensitive coordination bond

“…Because systems with this size are small enough to evade reticuloendothelial system and achieve tumor passive targeting by EPR effect, these systems may demonstrate prolonged blood circulation and offer the most effective distribution in certain tissues. 31 Evaluation of pH sensitivity. The release profile of DOX from TiO 2 @Fe 3 O 4 /PEI/DOX was investigated under simulated physiological conditions (PBS, pH 7.4) and in an acidic environment (PBS, pH 5.2) at 37 C. The release profile of DOX from DOX solution was also investigated as control group.…”

Synthesis and characterization of mesoporous magnetic nanocomposites wrapped with chitosan gatekeepers for pH-sensitive controlled release of doxorubicin

“…The experiment of in vitro release of DOX was conducted like previously reported [6,33]. First, nanocomposites with good dispersibility were spherical and the mesoporous of mSiO 2 could be clearly seen (Fig.…”

“…The peaks at 1408 and 1730 cm -1 could be assigned to the quinone and 13-carbonyl vibration of DOX ( Fig. 7b) [6], revealing the DOX has been loaded into Fe 3 O 4 @mSiO 2…”

“…Nanotechnology provides a flexible platform for the development of new nanomaterials that with distinct characteristics and functionalities suitable for applications in specific areas [1][2][3][4]. Of the nanomaterials studies, the magnetic nanomaterials especially the Fe 3 O 4 nanoparticles have been widely studied in the past decades because of their high potential applications in many areas especially in biomedical researches [5][6][7][8][9][10], due to their inherently ultra-fine size, low toxicity, biocompatibility and adequate magnetic properties [11]. These properties are fully exploited when they are used for drug delivery.…”