Facile Route to Ultraflat SAM‐Protected Gold Surfaces by “Amphiphile Splitting”

Gupta, Pooja; Loos, Katja; Korniakov, Alexander; Spagnoli, Chiara; Cowman, Mary K.; Ulman, Abraham

doi:10.1002/anie.200352249

Cited by 52 publications

(60 citation statements)

References 24 publications

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“…The thiols can intercalate into the mica sheets and thus reduce the binding to the gold. 28 The procedure has been shown to be also applicable to silicon templates. 12…”

Section: Templatesmentioning

confidence: 97%

As flat as it gets: ultrasmooth surfaces from template-stripping procedures

2012

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In an experimentally simple replica process, the natural flatness of mica or polished silicon wafers can be transferred to metal films, resulting in metal surfaces with topographic features in Angstrom dimensions over large areas. Two decades after its invention, the template-stripping process continues to appeal to scientists from diverse research backgrounds primarily due to its simplicity, cost-effectiveness and ability to yield high quality substrates and structures. This article introduces the basic construction process for template-stripped substrates, and reports on a variety of extensions of the process, including the generation of materials contrasts and the design of tailored topographies. It also highlights the use of such substrates in a variety of research fields in nanoscience and technology ranging from surface force measurement and high definition imaging to the self-assembly of model membranes and plasmonics.

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“…The thiols can intercalate into the mica sheets and thus reduce the binding to the gold. 28 The procedure has been shown to be also applicable to silicon templates. 12…”

Section: Templatesmentioning

confidence: 97%

As flat as it gets: ultrasmooth surfaces from template-stripping procedures

2012

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“…This disparity is especially problematic in electrochemistry because of the extraordinary sensitivity of heterogeneous electron-transfer reaction rates to the differences in the size and shape of extremely small (i.e., nanometer-sized or less) defects [41]. Other measures of adlayer properties (e.g., wettability [12,[42][43][44]) have also suffered from reproducibility challenges. To gain control over reproducibility, the underlying factors that affect the structural evolution of the adlayer must be more fully understood, placing a premium on the ability to trace structural details back to potentially subtle differences in preparative procedures.…”

Section: Introductionmentioning

confidence: 98%

Importance of reactant mass transfer in the reproducible preparation of self-assembled monolayers

Edwards

Bergren

Cox

et al. 2008

Journal of Electroanalytical Chemistry

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“…A tip-assisted AFM-based nanolithography technique has been used to fabricate DNA nanoarrays with high surface density (1-2 × 10 13 molecules/cm 2 ): using Si cantilevers (NSC36B Mikromasch (Mikromasch, Innovative Solutions Bulgaria Ltd., Sofia, Bulgaria), spring constant: 0.6 N/m) multiple nanografting assembled monolayers (NAM) of thiol-modified single-stranded DNA (ssDNA), named cF9, were prepared by serial AFM-based nanografting inside a self-assembled monolayer (SAM) of a top oligo ethylene glycol terminated alkanethiol, TOEG ((1-mercaptoundec-11-yl)hexa(ethyleneglycol), HS-(CH2)11-(OCH2CH2)6-OH from Sigma Aldrich) on ultraflat gold surfaces [22] following standard protocols reported earlier [9,23]. The DNA patches were obtained promoting the replacement of the TOEG molecules with the oligonucleotides by the AFM tip scanning an area of 1 µm × 1 µm or less at high force (about 100 nN) in the presence of a solution of thiolated ssDNA sequences (5 µM in TE buffer 1 M NaCl) at a scan rate of 2 Hz.…”

Section: Afm-based Nanoarraysmentioning

confidence: 99%

Miniaturized Aptamer-Based Assays for Protein Detection

et al. 2016

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Abstract:The availability of devices for cancer biomarker detection at early stages of the disease is one of the most critical issues in biomedicine. Towards this goal, to increase the assay sensitivity, device miniaturization strategies empowered by the employment of high affinity protein binders constitute a valuable approach. In this work we propose two different surface-based miniaturized platforms for biomarker detection in body fluids: the first platform is an atomic force microscopy (AFM)-based nanoarray, where AFM is used to generate functional nanoscale areas and to detect biorecognition through careful topographic measurements; the second platform consists of a miniaturized electrochemical cell to detect biomarkers through electrochemical impedance spectroscopy (EIS) analysis. Both devices rely on robust and highly-specific protein binders as aptamers, and were tested for thrombin detection. An active layer of DNA-aptamer conjugates was immobilized via DNA directed immobilization on complementary single-stranded DNA self-assembled monolayers confined on a nano/micro area of a gold surface. Results obtained with these devices were compared with the output of surface plasmon resonance (SPR) assays used as reference. We succeeded in capturing antigens in concentrations as low as a few nM. We put forward ideas to push the sensitivity further to the pM range, assuring low biosample volume (µL range) assay conditions.

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Facile Route to Ultraflat SAM‐Protected Gold Surfaces by “Amphiphile Splitting”

Cited by 52 publications

References 24 publications

As flat as it gets: ultrasmooth surfaces from template-stripping procedures

As flat as it gets: ultrasmooth surfaces from template-stripping procedures

Importance of reactant mass transfer in the reproducible preparation of self-assembled monolayers

Miniaturized Aptamer-Based Assays for Protein Detection

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