Ceria nanoparticles (CeO 2 NPs) have been synthesized by wet chemical technique using NH 4 OH precipitation method. CeO 2 NPs were prepared in presence of different concentration of the non-ionic surfactants Tween 20 and 80 of hydrophilic-lipophilic balance (HLB) of 16.7 and 15.0, respectively, to improve and controlling the size and shape of CeO 2 -NPs.The role of these surfactants in controlling the size of CeO 2 NPs has been discussed. The synthesized CeO 2 NPs were characterized by Fourier transform infrared (FT-IR), Raman spectroscopy, UV-Visible spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM). The TEM micrographs and XRD patterns confirmed the semi-spherical structure of CeO 2 NPs with ultrafine particle sizes ranging from 5.9 nm to 11.0 nm. FT-IR and Raman spectral analysis confirmed the identities of the oxides. In vitro antimicrobial studies show significant zone of inhibition against bacterial and fungal strains. The efficiency of CeO 2 NPs as a promising candidate in photocatalysis was evaluated using a methylene blue (MB) as organic contaminant dye irradiated with UV light from conventional high-efficiency low-pressure and low power (6 watt) mercury lamp is also established.
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