Facile synthesis of a ratiometric oxygen nanosensor for cellular imaging

Lu, Sisi; Xu, Wei; Zhang, Jinliang; Chen, Yiying; Xie, Lei; Yao, Qiuhong; Jiang, Yaqi; Wang, Yiru; Chen, Xi

doi:10.1016/j.bios.2016.06.050

Cited by 22 publications

(5 citation statements)

References 36 publications

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“…Examples of such phosphorescent probes that have demonstrated promise for in situ O 2 quantification are porphyrins − and transition-metal complexes. − Their excited states are long-lived (τ = hundreds of nanoseconds to microseconds) because of the forbidden nature of the T 1 → S 0 transition, which provides a large time window for collision with and quantification of O 2 . As such, there have been a growing number of reports demonstrating in vitro O 2 sensing using one- and two-photon lifetime imaging techniques (PLIM and PLM). − For example, these lifetime mapping techniques have been utilized in the quantification of O 2 in 2D cell cultures, 3D spheroids, and neurospheres using platinum(II) porphyrins. , Large dendritic platinum(II) porphyrins, which do not cross cellular membranes, have also been used to monitor the O 2 levels in cerebral blood vessels of a living animal, using a bespoke two-photon phosphorescence lifetime imaging setup. , Ratiometric probes, in which a dual-emissive species acts as both the calibrant and O 2 detector, have also been investigated for O 2 quantification. , These systems tend to utilize simpler instrumentation relying on the emission intensity measurements rather than lifetime mapping.…”

Section: Introductionmentioning

confidence: 99%

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Multimodal Probes: Superresolution and Transmission Electron Microscopy Imaging of Mitochondria, and Oxygen Mapping of Cells, Using Small-Molecule Ir(III) Luminescent Complexes

et al. 2017

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We describe an Ir(III)-based small-molecule, multimodal probe for use in both light and electron microscopy. The direct correlation of data between light- and electron-microscopy-based imaging to investigate cellular processes at the ultrastructure level is a current challenge, requiring both dyes that must be brightly emissive for luminescence imaging and scatter electrons to give contrast for electron microscopy, at a single working concentration suitable for both methods. Here we describe the use of Ir(III) complexes as probes that provide excellent image contrast and quality for both luminescence and electron microscopy imaging, at the same working concentration. Significant contrast enhancement of cellular mitochondria was observed in transmission electron microscopy imaging, with and without the use of typical contrast agents. The specificity for cellular mitochondria was also confirmed with MitoTracker using confocal and 3D-structured illumination microscopy. These phosphorescent dyes are part of a very exclusive group of transition-metal complexes that enable imaging beyond the diffraction limit. Triplet excited-state phosphorescence was also utilized to probe the O concentration at the mitochondria in vitro, using lifetime mapping techniques.

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Section: Introductionmentioning

confidence: 99%

“…9,18 Ratiometric probes, in which a dualemissive species acts as both the calibrant and O 2 detector, have also been investigated for O 2 quantification. 19,20 These systems tend to utilize simpler instrumentation relying on the emission intensity measurements rather than lifetime mapping.…”

Section: ■ Introductionmentioning

confidence: 99%

Multimodal Probes: Superresolution and Transmission Electron Microscopy Imaging of Mitochondria, and Oxygen Mapping of Cells, Using Small-Molecule Ir(III) Luminescent Complexes

et al. 2017

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“…Dissolved oxygen (DO) plays a critical role in regulating multiple processes involved in complex biological systems [1][2][3][4][5]. Meanwhile, hypoxia usually leads to illness.…”

Section: Introductionmentioning

confidence: 99%

Magnetically reusable and well-dispersed nanoparticles for oxygen detection in water

Cui

Wang

et al. 2021

Preprint

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In this study, we aimed to synthesize magnetically well-dispersed nanosensors for detecting dissolved oxygen (DO) in water, and explore their biological applications. Firstly, we synthesized two kinds of magnetic nanoparticle with average sizes of approximately 82 nm by one-step emulsion polymerization: polystyrene magnetic nanoparticles (Fe3O4@Os1-PS) and polymethylmethacrylate magnetic nanoparticles (Fe3O4@Os1-PMMA). Both types of nanoparticle present good dispersibility and fluorescence stability. The nanoparticles could be used as oxygen sensors that exhibited a high DO-sensitivity response in the range 0-39.30 mg/L, with a strong linear relationship. The nanoparticles have good magnetic properties, and so they could be recycled by magnet for further use. Recovered Fe3O4@Os1-PS still presented high stability after continued use in oxygen sensing for one month. Furthermore, Fe3O4@Os1-PS was employed for detecting the bacterial oxygen consumption of Escherichia coli (E-coli) to monitor the metabolism of bacteria. The results show that Fe3O4@Os1-PS provide high biocompatibility and non-toxicity. Polystyrene magnetic nanoparticles therefore present significant potential for application in biological oxygen sensing.

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“…The multi-emissive properties of these materials are beneficial for many uses such as OLEDs, − long-lifetime imaging probes, − and oxygen sensors. − Because the fluorescence and phosphorescence originate from a single dye or polymeric material, complications due to dye mixing are circumvented (i.e., fluorescent dye + phosphorescent dye). − The fluorescence, unquenched by oxygen, serves as an internal standard, while the oxygen-sensitive phosphorescence can report the relative oxygen concentration. Thus, ratiometric oxygen sensing is achievable with a single dye.…”

Section: Introductionmentioning

confidence: 99%

Amplified Heavy-Atom Free Phosphorescence from meta-Dimethoxy Difluoroboron β-Diketonate Charge-Transfer Materials

2019

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Difluoroboron β-diketonate materials are used for biomedical oxygen-sensing applications. These fluorophores show both fluorescence (F) and phosphorescence (P) when the dye is embedded in a polymer matrix. The fluorescence is insensitive to oxygen quenching, while the phosphorescence changes intensity depending on the oxygen concentration. This enables oxygen quantification by ratiometric methods with good spatial and temporal resolution. Here, we produce ratiometric-capable oxygen sensors without the need of a heavy atom. This is achieved by substituting methoxyl groups at the meta positions of an aromatic ring, dramatically influencing the electronic transitions of the fluorophore. Altering the aromatic group on the opposing ring tailors the absorption wavelength, luminescence colors, and relative fluorescence to phosphorescence ratio (F/P), which are important for oxygen sensing. The phosphorescence lifetimes are also hundreds of milliseconds long, achieving ultra-sensitivity to oxygen. This study presents the synthesis, optical characterization in solution and polylactide blends, and computational analysis of 3,5-dimethoxy-difluoroboron β-diketonate materials as oxygen-sensing materials.

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Facile synthesis of a ratiometric oxygen nanosensor for cellular imaging

Cited by 22 publications

References 36 publications

Multimodal Probes: Superresolution and Transmission Electron Microscopy Imaging of Mitochondria, and Oxygen Mapping of Cells, Using Small-Molecule Ir(III) Luminescent Complexes

Multimodal Probes: Superresolution and Transmission Electron Microscopy Imaging of Mitochondria, and Oxygen Mapping of Cells, Using Small-Molecule Ir(III) Luminescent Complexes

Magnetically reusable and well-dispersed nanoparticles for oxygen detection in water

Amplified Heavy-Atom Free Phosphorescence from meta-Dimethoxy Difluoroboron β-Diketonate Charge-Transfer Materials

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