Facile Synthesis of Tin Oxide Hollow Nanoflowers Interfering with Quorum Sensing-Regulated Functions and Bacterial Biofilms

Abstract

Monophasic tin dioxide nanoflowers (TONFs) assembled by rod-like nanostructures were prepared by coprecipitation method using tin chloride and ammonia precipitators, as the starting materials, without using any surfactants or templates. The structural, compositional, optical, and morphological properties of TONFs were investigated by XRD, FT-IR, UV-vis, SEM-EDX, and TEM techniques. Synthesized TONFs demonstrated inhibition of quorum sensing- (QS-) regulated virulence in pathogens, viz., Chromobacterium violaceum, Pseudomonas aeruginosa, and Serratia marcescens. Significant reduction in biofilm formation in all test pathogens was also observed which was further validated by CLSM images illustrating disturbed biofilm architecture. Vital functions like EPS, swarming motility, and cell surface hydrophobicity that contribute to successful biofilm formation were reduced after addition of sub-MICs of TONFs. Significant eradication of preformed biofilms was recorded upon addition of subinhibitory concentrations of TONFs in all test pathogens. The study highlights the broad-spectrum QS and biofilm inhibition by TONFs that can be exploited in future to protect food from contamination and combatting persistent drug-resistant infections.

Mitigation of acyl-homoserine lactone (AHL) based bacterial quorum sensing, virulence functions, and biofilm formation by yttrium oxide core/shell nanospheres: Novel approach to combat drug resistance

“…We recorded a significant reduction in biofilm biomass upon treatment with the core and Core/shell NSs. Our study is in accordance with previous reports that showed the reduction of biofilms in P. aeruginosa PAO1 by nanoparticles of zinc oxide 14,51 , tin oxide 52 and iron oxides 53 , silica nanoparticles 54 antimicrobials like doxycycline and ceftazidime 29,55 .

Figure 5Anti-biofilm activity of NSs. ( a ) Percent inhibition of biofilm formation of PAO1 by sub-MICs of core and core/shell NSs using microtitre plate assay.

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Bio-inspired facile fabrication of silver nanoparticles from in vitro grown shoots of Tamarix nilotica: explication of its potential in impeding growth and biofilms of Listeria monocytogenes and assessment of wound healing ability

“…27 Moreover, nanoparticles of iron oxide, tin oxide, gold, copper and organosilane have also been shown to inhibit biolm of L. monocytogenes. [42][43][44][45][46] This is probably the rst report on the inhibition of biolm formation in Listeria species by green synthesized AgNPs.…”

Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity

“…Exopolysaccharides (EPS) are an important constituent of the bacterial biofilm as they have a vital role in the maintenance of the 3D biofilm architecture and providing resistance against the action of antimicrobials and immune responses [ 48 ]. Several studies have highlighted that interference with EPS production leads to disturbed biofilm architecture and eventually reduces the development of resistance against antimicrobials [ 49 , 50 , 51 ]. In light of this, TA-AuNPs were evaluated for their ability to inhibit EPS production in S. marcescens as well as L. monocytogenes .…”